A5074058768- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Nuclear Structure and Function
- Cancer Diagnosis and Treatment
- Medical Imaging and Analysis
- RNA Research and Splicing
- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Synthesis and biological activity
- Click Chemistry and Applications
- Cancer Cells and Metastasis
- Dermatological and Skeletal Disorders
- Advanced NMR Techniques and Applications
- Infectious Diseases and Tuberculosis
- Receptor Mechanisms and Signaling
- Spine and Intervertebral Disc Pathology
- Adrenal and Paraganglionic Tumors
- Diagnosis and treatment of tuberculosis
- Hippo pathway signaling and YAP/TAZ
- Sarcoidosis and Beryllium Toxicity Research
- Health Systems, Economic Evaluations, Quality of Life
Max Planck Institute of Molecular Physiology
2000-2022
Danube Private University
2022
Alexion Pharmaceuticals (United States)
2014-2022
AstraZeneca (United States)
2022
Alexion Pharma (Switzerland)
2015-2016
Alexion Pharmaceuticals (France)
2016
Cheshire Medical Center
2014
Max Planck Society
1999-2009
Max Planck Institute for Polymer Research
2009
Austrian Institute of Technology
2009
Transcriptional enhanced associate domain (TEAD) transcription factors together with coactivators and corepressors modulate the expression of genes that regulate fundamental processes, such as organogenesis cell growth, elevated TEAD activity is associated tumorigenesis. Hence, novel modulators methods for their identification are in high demand. We describe development a new "thiol conjugation assay" small molecules bind to central pocket. The assay monitors prevention covalent binding...
Recombinant monoclonal antibody charge heterogeneity has been commonly observed as multiple bands or peaks when analyzed by charge-based analytical methods such isoelectric focusing electrophoresis and cation anion exchange chromatography. Those variants have separated some of the above-mentioned used for detailed characterization. The utility a combination OFFGEL fractionation weak chromatography to separate recombinant was demonstrated in current study. Charge were into various fractions...
Abstract Profiling approaches have been increasingly employed for the characterization of disease‐relevant phenotypes or compound perturbation as they provide a broad, unbiased view on impaired cellular states. We report that morphological profiling using cell painting assay (CPA) can detect modulators de novo pyrimidine biosynthesis and dihydroorotate dehydrogenase (DHODH) in particular. The CPA differentiate between impairment folate metabolism, which both affect nucleotide pools....
Matching the membrane: The lipid modification of human N-Ras proteins can adapt remarkably to host membrane by varying its length between 8.7 Å and 15.5 Å, as be seen in 2H NMR spectra deuterated derivatives (see picture). In raft-forming biological membranes, Ras is found liquid disordered phase well boundary liquid-crystalline raft domains. Detailed facts importance specialist readers are published "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They...
Many proteins involved in signal transduction are equipped with covalently attached lipid chains providing a hydrophobic anchor targeting these molecules to membranes. Despite the considerable biological significance of this membrane binding mechanism for 5−10% all cellular proteins, date very little is known about structural and dynamical features lipidated domains. Here we report first comprehensive study molecular dynamics C-terminus membrane-associated full-length Ras protein determined...
Charge variants, especially acidic charge of recombinant monoclonal antibodies have been challenging to fully characterize despite the fact that several posttranslational modifications already identified. The species a antibody were collected using weak cation exchange (WCX)-10 chromatography and characterized by LC-MS at multiple levels. In this study, methionine oxidation asparagine deamidation are only two identified in species. Incubation main chromatographic peak with hydrogen peroxide...
The cytoplasmic disassembly of Ran.GTP.importin and Ran.GTP.exportin. cargo complexes is an essential step in the corresponding nuclear import export cycles. It has previously been shown that such can be mediated by RanBP1 presence RanGAP. pore complex protein RanBP2 (Nup358) contains four Ran-binding domains (RanBDi) might function like RanBP1. We used biophysical assays based on fluorescence-labeled probes surface plasmon resonance to investigate dynamic interplay Ran its GDP-...
A recombinant monoclonal antibody with trisulfide bonds and cysteinylation was thoroughly characterized in the current study. Trisulfide were first detected when analyzed by LC-MS to determine molecular weights of intact its F(ab')2 fragment generated from IdeS digestion. analysis nonreduced tryptic peptides indicated are associated interchain disulfide both isoform A/B is only isoform. low percentage between light chain heavy bond forms. While majority hinge region peptide that involves...
Solid-state NMR spectroscopy was used to determine a structural model of the backbone lipid anchor membrane-bound N-Ras protein. The fully functional lipid-modified protein obtained by ligating expressed water-soluble N terminus with chemically synthesized 13C-labeled lipidated peptide. After signals had been assigned correlation experiments, calculated from torsion angles derived 1H and 13C chemical-shift data.
Fully functional lipid-modified Ras proteins suitable for the study of Ras–membrane interactions and embodying exclusively native amide bonds can be synthesized in preparative amounts by means Expressed Protein Ligation.
A two-layer gold surface is developed for use with surface-enhanced infrared absorption spectroscopy (SEIRAS) consisting of a conducting underlayer onto which Au nanoparticles (AuNPs) are grown by self-catalyzed electroless deposition. AuNPs on protruding substructures the 25 nm thin underlayer. The enhancement factor controlled growth conditions. Cytochrome c adsorbed to self-assembled monolayer mercaptoethanol used as reference system. Under optimum conditions absorbance amide I band...
Asparagine deamidation in the complementarity determining regions of recombinant monoclonal antibodies has been extensively studied and shown to have a negative impact on antigen binding. Those asparagine residues are typically exposed thus higher tendency toward deamidation, depending also local structure environmental factors such as temperature pH. Deamidation rates products susceptible residue antibody free solution or antibody–antigen complex were studied. The results demonstrated that...