A5012016106- Digestive system and related health
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Neonatal Respiratory Health Research
- 3D Printing in Biomedical Research
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Helicobacter pylori-related gastroenterology studies
- Cystic Fibrosis Research Advances
- RNA modifications and cancer
- Infant Nutrition and Health
- Evolutionary Algorithms and Applications
- Pancreatic function and diabetes
- Neonatal and Maternal Infections
- Renal and related cancers
- Pluripotent Stem Cells Research
- Wnt/β-catenin signaling in development and cancer
- Bacterial Infections and Vaccines
- Barrier Structure and Function Studies
- Lymphatic System and Diseases
University of California, San Francisco
2019-2024
University of San Francisco
2021
University of California, San Diego
2015
San Diego State University
2015
Bacterial meningitis is a serious infection of the CNS that results when blood-borne bacteria are able to cross blood-brain barrier (BBB). Group B Streptococcus (GBS) leading cause neonatal meningitis; however, molecular mechanisms regulate bacterial BBB disruption and penetration not well understood. Here, we found human brain microvascular endothelial cells (hBMECs) with GBS other meningeal pathogens in induction host transcriptional repressor Snail1, which impedes expression tight...
The gastrointestinal tract relies on the production, maturation, and transit of mucin to protect against pathogens lubricate epithelial lining. Although molecular cellular mechanisms that regulate production movement are beginning be understood, upstream signals contribute regulation remain unclear. Here, we report inflammatory cytokine tumor necrosis factor (TNF), generated by epithelium, contributes homeostasis regulating both cell differentiation cystic fibrosis transmembrane conductance...
Abstract Intracellular pH dynamics is increasingly recognized to regulate myriad cell behaviors. We report a finding that intracellular also regulates adult stem lineage specification. identify an gradient in mouse small intestinal crypts, lowest crypt cells and increasing along the column. Disrupting this by inhibiting H + efflux Na /H exchanger 1 abolishes budding blocks differentiation of Paneth cells, which are rescued with exogenous WNT. Using single-cell RNA sequencing tracing we...
Abstract A key aspect of nutrient absorption is the exquisite division labor across length small intestine, with individual classes micronutrients taken up at different positions. For millennia, intestine was thought to comprise three segments indefinite borders: duodenum, jejunum, and ileum. By examining fine-scale longitudinal segmentation mouse human intestines, we identified transcriptional signatures upstream regulatory factors that define five domains absorption, distinct from...
ABSTRACT BACKGROUND AND AIMS Methotrexate is an important tool in the arsenal of oncologists, gastroenterologists, and rheumatologists. At low doses it induces intestinal barrier dysfunction that may induce side effects such as gastrointestinal discomfort liver injury. Previous studies suggest lactoferrin can improve function a variety contexts. This study set out to determine mechanism methotrexate induced assess effect other components human breast milk on this dysfunction. METHODS Using...
Summary Intracellular pH (pHi) dynamics is increasingly recognized to regulate myriad cell behaviors, including proliferation, migration, differentiation, and transformation. Here we report a new finding that pHi also regulates adult stem lineage specification. In mouse small intestinal organoids, identify gradient along the crypt axis, lower at base higher toward villus, find dissipating this by inhibiting Na + -H exchanger 1 (NHE1) activity genetically or pharmacologically abolishes...