Abstract Background: The role of environmental surface contamination in the propagation norovirus outbreaks is unclear. An outbreak acute gastroenteritis was reported among residents a 240-bed veterans long-term-care facility. Objectives: To identify likely mode transmission, to characterize risk factors for illness, and evaluate this outbreak. Methods: investigation conducted illness employees. Stool vomitus samples were tested by reverse transcription polymerase chain reaction (RT-PCR)....

The nontherapeutic use of antibiotics in swine feed can select for antibiotic resistance enteric bacteria. Leaking waste storage pits and the land-application manure result dispersion resistant bacteria to water sources. However, there are few data comparing levels manure-impacted sources versus unaffected sources.The goal this study was analyze surface groundwater situated up down gradient from a facility antibiotic-resistant enterococci other fecal indicators.Surface samples (n = 28) were...

The use of nontherapeutic levels antibiotics in swine production can select for antibiotic resistance commensal and pathogenic bacteria swine. As a result, retail pork products, as well surface groundwaters contaminated with waste, have been shown to be sources human exposure antibiotic-resistant bacteria. However, it is unclear whether the air within operations also serves source bacterial pathogens. To investigate this issue, we sampled concentrated feeding operation an all-glass impinger....

Controlled environment agriculture (CEA) is a growing industry for the production of leafy vegetables and fresh produce in general. Moreover, CEA potentially desirable alternative system, as well risk management solution food safety challenges within industry. Here, we will focus on hydroponic vegetable (including lettuce, spinach, microgreens, herbs), which can be categorized into six types: (1) nutrient film technique (NFT), (2) deep water raft culture (DWC), (3) flood drain, (4)...

Abstract Surfaces contaminated with enveloped viruses, such as severe acute respiratory syndrome coronavirus 2 and influenza virus, can potentially spread illness via hand contact. Often, the efficacy of hygiene interventions relies on virus recovery from hands. However, bacteriophage phi6 (Φ6), a recommended surrogate for entire hands using ASTM E2011-21 standard has not been optimized. For Φ6 hands, three eluents [lysogeny broth (LC), tryptic soy (TSB), 1.5% beef extract (BE)] methods...

Three organic wastewater compounds (OWCs) were evaluated in theory and practice for their potential to trace sewage-derived microbial contaminants surface waters. The underlying hypothesis was that hydrophobic OWCs outperform caffeine as a chemical tracer, due sorptive association with suspended microorganisms representing particulate carbon (POC). Modeling from first principles (ab initio) of OWC sorption POC under environmental conditions suggested an increasing predictive power: (0.2%...

Tangential-flow ultrafiltration was optimized for the recovery of Escherichia coli, Enterococcus faecalis, Clostridium perfringens spores, bacteriophages MS2 and PRD1, murine norovirus, poliovirus seeded into 100-liter surface water (SW) drinking (DW) samples. SW DW collected from two treatment plants were then evaluated human enteric viruses.

An estimated 884 million people worldwide do not have access to an improved drinking water source, and the microbial quality of these sources is often unknown. In this study, a combined tangential flow, hollow fiber ultrafiltration (UF), real-time PCR method was applied large volume (100 L) groundwater ( N = 4), surface 9), finished (i.e., receiving treatment) 6) samples for evaluation human enteric viruses bacterial indicators. Human including norovirus GI GII, adenovirus, polyomavirus were...

Contamination of food contact surfaces with pathogens is considered an important vehicle for the indirect transmission food-borne diseases. Five different cleaning cloths were assessed ability to remove viruses from (stainless steel surface and nonporous solid surface) transfer back these surfaces. Cleaning evaluated include two cellulose/cotton cloths, one microfiber cloth, nonwoven cotton terry bar towel. Four viral surrogates (murine norovirus [MNV], feline calicivirus [FCV],...

Journal Article Physicochemical stability profile of Tulane virus: a human norovirus surrogate Get access S.E. Arthur, Arthur Department Food Science Center for Safety University Arkansas Fayetteville AR USA Search other works by this author on: Oxford Academic Google Scholar K.E. Gibson Correspondence Kristen E. Gibson, 2650 N Young Ave, Fayetteville, 72704, USA. E‐mail: keg005@uark.edu Applied Microbiology, Volume 119, Issue 3, 1 September 2015, Pages 868–875,...

Tangential flow ultrafiltration (UF) was used to concentrate and recover bacterial indicators enteric viruses from 100 liters of groundwater (GW; n = 10) surface water (SW; 11) samples collected in Lower Yakima Valley, WA. Human bovine were analyzed SW GW concentrates by real-time PCR using integrated inhibition detection.

Purchasing fresh and local produce at farmers markets has seen an increasing trend over the past decade. However, with this rise in popularity food safety challenges need to be recognized addressed. Farmers market managers play a significant role ensuring that vendors implement practices market. Thus, study investigated perceptions of Texas Arkansas. A total 123 participants were surveyed, including 38 85 vendors. The survey included series questions determine gaps vendor manager knowledge...