A5062988185- Identification and Quantification in Food
- Environmental DNA in Biodiversity Studies
- Microbial Community Ecology and Physiology
- Isotope Analysis in Ecology
- Fish Ecology and Management Studies
- Coral and Marine Ecosystems Studies
- Species Distribution and Climate Change
- Water Quality Monitoring Technologies
- Data Quality and Management
- Fisheries and Aquaculture Studies
- Marine Ecology and Invasive Species
- Food Supply Chain Traceability
- Fish Biology and Ecology Studies
- Genomics and Phylogenetic Studies
- Research Data Management Practices
- Fish biology, ecology, and behavior
- Genetic diversity and population structure
- Marine and fisheries research
- Marine Bivalve and Aquaculture Studies
- Semantic Web and Ontologies
- Energy and Environmental Systems
- Marine Biology and Ecology Research
- Animal Ecology and Behavior Studies
- Scientific Computing and Data Management
- Ichthyology and Marine Biology
University of Salento
2013-2024
National Research Council
2013-2024
Amsterdam University of the Arts
2024
University of Amsterdam
2024
Hellenic Centre for Marine Research
2024
University of Hull
2017-2023
Water Research Institute
2023
University of Bari Aldo Moro
2023
University of Zurich
2021
Chiang Mai University
2021
Efficient DNA extraction is fundamental to molecular studies. However, commercial kits are expensive when a large number of samples need be processed. Here we present simple, modular and adaptable ‘toolkit’ for the isolation high purity from multiple sample types (modular universal method or Mu-DNA). We compare performance our that widely used across range soil, stool, tissue water samples. Mu-DNA produced extractions similar higher yield kits. As proof principle, carried out replicate fish...
The sampling of environmental DNA (eDNA) coupled with cost-efficient and ever-advancing sequencing technology is propelling changes in biodiversity monitoring within aquatic ecosystems. Despite the increasing number eDNA metabarcoding approaches, ability to quantify species biomass abundance natural systems still not fully understood. Previous studies have shown positive but sometimes weak correlations between estimates from data conventional capture methods. As both methods independent...
Although the spread of sushi restaurants in European Union and United States is a relatively new phenomenon, they have rapidly become among most popular food services globally. Recent studies indicate that can be associated with very high levels (>70%) fish species substitution. Based on indications seafood retail sector may currently under better control than its North American counterpart, here we investigated labelling accuracy bars across England. We used COI barcoding gene to screen...
Abstract Large tropical and subtropical rivers are among the most biodiverse ecosystems worldwide, but also suffer from high anthropogenic pressures. These hitherto subject to little or no routine biomonitoring, which would be essential for identification of conservation areas importance. Here, we use a single environmental DNA multi-site sampling campaign across 200,000 km 2 Chao Phraya river basin, Thailand, provide key information on fish diversity. We found total 108 taxa identified...
Biomonitoring of complex heterogeneous environments is highly challenging. Fish in deep water bodies occupy different habitats, so a combination survey methods has traditionally been used. Environmental DNA (eDNA) metabarcoding novel monitoring tool that can overcome spatial heterogeneity sensitive and entirely non-invasive manner. However, taxon detection probability dependent on environmental variables. In this study, three reservoirs were sampled two seasons using spatiotemporally...
Abstract: Background Environmental DNA (eDNA) metabarcoding is a promising tool for rapid, non‐invasive biodiversity monitoring. Aims In this study, eDNA applied to explore the spatial and temporal distribution of fish communities in two aquaculture ponds evaluate detection sensitivity low‐density species alongside highly abundant species. Materials & Methods This study was carried out at artificially stocked with high density following introduction removal rare Results Discussion When...
Abstract Determining the timing and location of fish reproductive events is crucial for implementation correct management conservation schemes. Conventional methods used to monitor these are often unable assess spawning activity directly or can be invasive therefore problematic. This especially case when threatened populations study subject, such as Arctic charr ( Salvelinus alpinus L.) in Windermere (Cumbria, UK). have been studied this lake since 1940s, locations characteristics grounds...
Environmental DNA (eDNA) metabarcoding is transforming biodiversity monitoring in aquatic environments. Such an approach has been developed and deployed for lake fish communities Great Britain, where the method repeatedly shown a comparable or better performance than conventional approaches. Previous analyses indicated that 20 water samples per are sufficient to reliably estimate species richness, but it unclear how reduced eDNA sampling effort affects other estimates metrics. As number of...
Addressing the challenges posed by Non Indigenous Species (NIS) demands coordinated efforts across terrestrial, freshwater, and marine ecosystems. In this study, we used Italian research network as a case study to identify evaluate measures designed enhance NIS through structured, interactive, expert-driven strategy. The process began with comprehensive evaluation of ongoing activities, methodologies, specific needs. This assessment informed development an initial set proposals, which were...
Abstract The sampling of environmental DNA (eDNA) coupled with cost-efficient and ever-advancing sequencing technology is propelling changes in biodiversity monitoring within aquatic ecosystems. Despite the growth metabarcoding approaches, ability to quantify species biomass abundance natural systems remains a major challenge. Few studies have examined association between eDNA data inferred by whole-organism sampling, mesocosms or mock communities, interpretation reads as measure number...
It is well known that temporal fluctuations in small populations deeply influence evolutionary potential. Less whether can the potentials of species with large census sizes. Here, we estimated genetic population parameters from as survey polymorphic microsatellite DNA loci archived otoliths Adriatic European anchovy (Engraulis encrasicolus), a fish sizes supports numerous local fisheries. Stocks have fluctuated greatly over past few decades, and fishery collapsed 1987. Our results show...
Morphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management seafood labelling. comprises four species, commonly known as ‘redfish’, but little is about the number, identity labelling accuracy redfish species sold across Europe. We used a molecular approach to identify from ‘blind’ specimens evaluate performance Barcode Life (BOLD) Genbank...
Abstract Determining the timing and location of fish reproductive events is crucial for implementation correct management conservation schemes. Conventional methods used to monitor these are often unable assess spawning activity directly or can be invasive therefore problematic. This especially case when threatened populations study subject, such as Arctic charr ( Salvelinus alpinus L.) in Windermere (Cumbria, UK). have been studied this lake since 1940s, locations characteristics grounds...
An advanced characterization of the trophic niche non-indigenous species (NIS) may provide useful information on their ecological impact invaded communities. Here, we used carbon and nitrogen stable isotopes to estimate pairwise overlaps between native consumers in winter food web Lake Trasimeno (central Italy). Overall, a relatively low overlap isotopic niches was observed NIS species. The only exception Louisiana crayfish Procambarus clarkii, which showed high diffuse with other...
Papers including articles that are produced because of the activities LifeWatch ERIC, in context its second implementation period (2022 - 2026) and through new Strategic Working Plan, published this special collection. The include data papers, papers describing development functioning analytical services any other research outcome, either by ERIC or collaboration with Research Infrastructure, global aggregator legal entity.
Freshwater biodiversity is critically affected by human modifications of terrestrial land use and cover (LULC). Yet, knowledge the spatial extent magnitude LULC-aquatic linkages still surprisingly limited, impeding implementation optimal management strategies. Here, we compiled fish diversity data across a 160,000-km2 subtropical river catchment in Thailand characterized exceptional yet intense anthropogenic alterations, attributed species richness community composition to contemporary LULC...
There is a growing recognition that an advanced understanding of the trophic characteristics invasive consumer can provide important information on its ecological impact. In recent years, blue swimming crab Portunus segnis, one earliest Lessepsian invaders, has considerably expanded distribution range in Mediterranean Sea, yet, habits invaded areas remain scarcely investigated. this study, we used carbon and nitrogen stable isotopes analysis (SIA) to determine position isotopic niche...
The characterisation of functional traits non-indigenous and invasive species is crucial to assess their impact within invaded habitats. Successful biological invasions are often facilitated by the generalist diet invaders which can modify trophic position adapt new ecosystems determining changes in structure functioning. Invasive crustaceans an illustrative example such mechanisms since habits determine important ecological impacts on aquatic food webs. Atlantic blue crab Callinectessapidus...
Abstract Environmental DNA (eDNA) metabarcoding is largely used to survey aquatic communities, but can also provide data on terrestrial taxa utilising habitats. However, the entry, dispersal, and detection of species’ within waterbodies understudied. We evaluated eDNA pond water for monitoring semi-aquatic, ground-dwelling, arboreal mammals, examined spatiotemporal variation in mammal signals using experiments captive wild conditions. selected nine focal species conservation management...
Environmental DNA (eDNA) metabarcoding is transforming biodiversity monitoring in aquatic environments where the method has repeatedly shown comparable or better performance than conventional approaches to fish monitoring. This been developed and deployed, primarily using shoreline sampling during winter months, across 101 lakes Great Britain alone, covering a wide spectrum of lake types ecological quality. Previous analyses on subset these indicated that 20 water samples per are sufficient...
Abstract Freshwater biodiversity is critically affected by human modifications of terrestrial land use and cover (LULC) 1,2 . Yet, knowledge the spatial extent magnitude LULC-aquatic linkages still surprisingly limited, impeding implementation optimal management strategies 3 Here, we compiled fish diversity data across a 160,000-km 2 subtropical river catchment in Thailand characterized exceptional 4 yet intense anthropogenic alterations 5 , attributed species richness community composition...
LifeWatch ERIC has embarked on its new destination towards upgrading and (co-)constructing Infrastructure as a response to the needs of target communities stakeholders. Through an industrialisation process, all independent data, software components, publications other types research products contributed by Member Countries will be consolidated integrated enable collaborative development. The Technology Readiness Level raised level 9. This process is described in Strategic Working Plan second...
Strengthening semantic interoperability within interdisciplinary contexts is crucial to facilitate the discovery, (re)use and integration of scientific data by diverse research communities. The Italian Integrated Environmental Research Infrastructures System (ITINERIS) project coordinates a network national nodes from 22 (RIs) operating mainly in environmental domain it represents perfect interdisciplinarity model foster implementation FAIR semantics.  main goal ITINERIS build hub...