A5069115306- Pancreatic function and diabetes
- Diabetes and associated disorders
- Diet, Metabolism, and Disease
- Metabolism, Diabetes, and Cancer
- Mosquito-borne diseases and control
- Regulation of Appetite and Obesity
- Viral Infections and Vectors
- Endoplasmic Reticulum Stress and Disease
- Adipokines, Inflammation, and Metabolic Diseases
- Diabetes Treatment and Management
- Acute Lymphoblastic Leukemia research
- Genetics and Neurodevelopmental Disorders
- Glutathione Transferases and Polymorphisms
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Polysaccharides Composition and Applications
- Viral Infections and Outbreaks Research
- Diabetes Management and Research
- Adipose Tissue and Metabolism
- Childhood Cancer Survivors' Quality of Life
- Phagocytosis and Immune Regulation
- Hormonal and reproductive studies
- Angiogenesis and VEGF in Cancer
- Transgenic Plants and Applications
- Ginseng Biological Effects and Applications
- Traditional Chinese Medicine Analysis
Mahidol University
2014-2024
Siriraj Hospital
2014-2024
The University of Melbourne
2002-2005
Vanderbilt University
2002
The ATP-sensitive potassium channel is a key molecular complex for glucose-stimulated insulin secretion in pancreatic β cells. In humans, mutations either of the two subunits this channel, sulfonylurea type 1 receptor (Sur1) or Kir6.2, cause persistent hyperinsulinemic hypoglycemia infancy. We have generated and characterized <i>Sur1</i> null mice. Interestingly, these animals remain euglycemic large portion their life despite constant depolarization membrane, elevated cytoplasmic free...
Context: Six maturity onset diabetes of the young (MODY) genes have been discovered to date but account for a small proportion MODY among Asians, suggesting existence other in this racial group.
Type 2 diabetes is characterized by a susceptibility to β-cell failure. However, subjects at risk of developing type diabetes, such as those with obesity or family history have been shown display hyperinsulinemia. Although this hyperinsulinemia may be an adaptive response insulin resistance, the possibility that hypersecretion primary defect has not thoroughly investigated. The DBA/2 mouse model pancreatic islet susceptibility. Unlike resistant C57BL/6 strain, fails when stressed resistance...
Type 2 diabetes is characterized by islet dysfunction resulting in hyperglycemia, which can then lead to further deterioration function. A possible mechanism for hyperglycemia-induced the accumulation of advanced glycation end products (AGE). The DBA/2 mouse develops pancreatic when exposed a high glucose environment and/or obesity-induced insulin resistance. To determine biochemical cause dysfunction, and C57BL/6 control islets were incubated 11.1 mM or 40 absence presence AGE inhibitor...
Male hypogonadism associates with type 2 diabetes, and T can protect pancreatic β-cells from glucotoxicity. However, the protective mechanism is still unclear. This study thus aims to examine antiapoptotic of in β cells cultured high-glucose medium. (0.0005-2 μg/mL) was added INS-1 basal glucose or media. Then cellular apoptosis, oxidative stress, cell viability were measured. Endoplasmic reticulum (ER) stress markers sensors protein (B-cell lymphoma 2) investigated by real-time PCR Western...
Hyperglycemia stimulates several pathways to induce pancreatic β-cell apoptosis. In our previous study by mRNA analysis, we demonstrated that B-cell translocation gene 2 (BTG2) expression was up-regulated in INS-1 cells cultured under high glucose conditions, but this effect reversed estrogen. the present study, BTG2 and protein expressions both mouse islets increased conditions compared those basal while presence of estrogen, decreased. SiRNA-BTG2 significantly reduced cell apoptosis,...
Hypogonadism in men is associated with an increased incidence of type 2 diabetes. Supplementation testosterone has been shown to protect pancreatic β-cell against apoptosis due toxic substances including streptozotocin and high glucose. One the pathological mechanisms glucose-induced induction local rennin–angiotensin–aldosterone system (RAAS). The role regulation RAAS still unknown. This study aims investigate protective action glucotoxicity-induced via alteration pathway. Rat insulinoma...
Abstract Steroid-induced diabetes is a well-known metabolic side effect of long-term use glucocorticoid (GC). Our group recently demonstrated dexamethasone-induced pancreatic β-cell apoptosis via upregulation TRAIL and death receptor (DR5). Genistein protects against induced by toxic agents. This study aimed to investigate the cytoprotective genistein in cultured rat insulinoma (INS-1) cell line isolated mouse islets. In absence genistein, was associated with TRAIL, DR5, superoxide...
Vascular endothelial growth factor (VEGF) is one of the key modulators angiogenesis. The highly polymorphic promoter and 5' untranslated region VEGF have been associated with susceptibility to aggressiveness several types cancer. To examine functional role polymorphisms at -634 -1498 positions, mRNA protein in breast cancer tissues were analyzed by quantitative polymerase chain reaction immunohistochemistry. A dual-luciferase assay was performed determine activity. VEGF-634CC genotype...
Long-term medication with dexamethasone – a synthetic glucocorticoid (GC) drug results in hyperglycemia, or steroid-induced diabetes. Although recent studies revealed that directly induces pancreatic β-cell apoptosis, its molecular mechanisms remain unclear. In our initial analysis of mRNA transcripts, we discovered the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) pathway may be involved dexamethasone-induced apoptosis. present study, mechanism apoptosis through TRAIL was...
Prolonged administration of dexamethasone, a potent anti-inflammatory drug, can lead to steroid-induced diabetes. Imatinib, medication commonly prescribed for chronic myeloid leukemia (CML), has been shown improve diabetes in CML patients. Our recent study demonstrated that dexamethasone induces pancreatic β-cell apoptosis by upregulating the expression tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its receptor, death receptor 5 (DR5). We hypothesized imatinib may...
Glucocorticoids (GCs) are known to stimulate pancreatic beta (β)-cell apoptosis via several mechanisms, including oxidative stress. Our previous study suggested an increase in dexamethasone-induced β-cell a reduction of glutathione S-transferase P1 (GSTP1), which is antioxidant enzyme. Imatinib, tyrosine kinase inhibitor, also exerts effect. This aims test our hypothesis that imatinib would prevent induced by dexamethasone increased GSTP1 expression and reduced results revealed significantly...
Gastric motility disturbance is commonly found in long-standing hyperglycemia. Both delayed and rapid gastric emptying has been reported diabetes. However, very few studies have followed the changes during disease progression diabetes because of technical limitations. 13C-Acetic acid breath test a validated method which non-invasive can be used repeatedly or serially to evaluate animal. We investigated different stages using 13C-acetic test, as well its related mechanisms involving...