A5089834834- Neuroscience of respiration and sleep
- High Altitude and Hypoxia
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Cancer, Hypoxia, and Metabolism
- Nerve injury and regeneration
- Hemoglobin structure and function
- Neurogenesis and neuroplasticity mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Cardiac electrophysiology and arrhythmias
- Nitric Oxide and Endothelin Effects
- Neuroscience and Neural Engineering
- Heart Rate Variability and Autonomic Control
- Adipose Tissue and Metabolism
- Sleep and Wakefulness Research
- Autism Spectrum Disorder Research
- Neurological disorders and treatments
- Pluripotent Stem Cells Research
- Adrenal and Paraganglionic Tumors
- Neuroinflammation and Neurodegeneration Mechanisms
- Respiratory Support and Mechanisms
- Nuclear Receptors and Signaling
- Nicotinic Acetylcholine Receptors Study
- Eicosanoids and Hypertension Pharmacology
Hospital Universitario Virgen del Rocío
2016-2025
Universidad de Sevilla
2016-2025
Instituto de Biomedicina de Sevilla
2016-2025
Biomedical Research Networking Center on Neurodegenerative Diseases
2016-2025
Consejo Superior de Investigaciones Científicas
2008-2022
Centro de Investigación Biomédica en Red
2008-2016
Women & Children's Hospital of Buffalo
2015
Biomedical Research Institute
2015
UCLA Medical Center
2015
University of California, Los Angeles
2015
The ionic currents of carotid body type I cells and their possible involvement in the detection oxygen tension ( P O 2 ) arterial blood are unknown. electrical properties these were studied with whole-cell patch clamp technique, hypothesis that conductances can be altered by changes was tested. results show have voltage-dependent sodium, calcium, potassium channels. Sodium calcium unaffected a decrease from 150 to 10 millimeters mercury, whereas, same experimental protocol, reversibly...
Abstract Induced pluripotent stem cells (iPSC) offer an unprecedented opportunity to model human disease in relevant cell types, but it is unclear whether they could successfully age‐related diseases such as Parkinson's (PD). Here, we generated iPSC lines from seven patients with idiopathic PD (ID‐PD), four familial associated the G2019S mutation Leucine‐Rich Repeat Kinase 2 ( LRRK2 ) gene (LRRK2‐PD) and age‐ sex‐matched healthy individuals (Ctrl). Over long‐time culture, dopaminergic...
1. In nine alert chronically prepared cats the activity of 177 neurons was recorded in prepositus nucleus during either spontaneous eye movement or that induced by natural vestibular and optokinetic stimulation. 2. 116 cells, position and/or velocity precisely unequivocally encoded whatever origin movement. These cells were separated into different populations according to variable directionality neuronal response. The firing rates remaining 61 loosely related movements because a variety...
Abstract Alveolar epithelial type 1 (AT1) cells are necessary to transfer oxygen and carbon dioxide between the blood air. 2 (AT2) serve as a partially committed stem cell population, producing AT1 during postnatal alveolar development repair after influenza A SARS-CoV-2 pneumonia 1–6 . Little is known about metabolic regulation of fate lung cells. Here we report that deleting mitochondrial electron transport chain complex I subunit Ndufs2 in mouse gestation led death development. Affected...
We have investigated the changes of cytosolic [Ca2+] and secretory activity in single glomus cells dispersed from rabbit carotid bodies during exposure to solutions with variable O2 tension (Po2). In normoxic conditions (Po2 = 145 mmHg; 1 mmHg 133 Pa), intracellular was 58 +/- 29 nM, switching low Po2 (between 10 60 mmHg) led a reversible increase up 800 nM. The response hypoxia completely disappeared after removal external Ca2+ or addition 0.2 mM Cd2+ solution. These same also abolished...
The hypothesis that changes in environmental O2 tension (pO2) could affect the ionic conductances of dissociated type I cells carotid body was tested. Cells were subjected to whole-cell patch clamp and currents recorded a control solution with normal pO2 (pO2 = 150 mmHg) 3-5 min after exposure same lower pO2. Na Ca unaffected by lowering 10 mmHg, however, all studied (n 42) hypoxia produced reversible reduction K current. In 14 exposed mmHg peak current amplitude decreased 35 +/- 8% value....
Cell culture studies have implicated the oxygen-sensitive hypoxia-inducible factor (HIF) prolyl hydroxylase PHD3 in regulation of neuronal apoptosis. To better understand this function vivo, we created PHD3−/− mice and analyzed phenotype. Reduced apoptosis superior cervical ganglion (SCG) neurons cultured from is associated with an increase number cells SCG, as well adrenal medulla carotid body. Genetic analysis by intercrossing HIF-1a+/− HIF-2a+/− demonstrated interaction HIF-2α but not...
The epigenomic landscape of Parkinson's disease (PD) remains unknown. We performed a genomewide DNA methylation and transcriptome studies in induced pluripotent stem cell (iPSC)-derived dopaminergic neurons (DAn) generated by reprogramming somatic skin cells from patients with monogenic LRRK2-associated PD (L2PD) or sporadic (sPD), healthy subjects. observed extensive changes DAn, RNA expression, which were common L2PD sPD. No significant differences present parental cells, undifferentiated...
HIF2α-mediated gene expression enables the carotid body to respond acute hypoxia.
Effective T cell-mediated immune responses require the proper allocation of metabolic resources to sustain growth, proliferation, and cytokine production. Epigenetic control genome also governs cell transcriptome lineage commitment maintenance. Cellular programs interact with epigenetic regulation by providing substrates for covalent modifications chromatin. By using complementary genetic, epigenetic, approaches, we revealed that tricarboxylic acid (TCA) cycle flux fueled biosynthetic...