A5079675144- Phagocytosis and Immune Regulation
- Heme Oxygenase-1 and Carbon Monoxide
- Drug Solubulity and Delivery Systems
- COVID-19 Clinical Research Studies
- Erythrocyte Function and Pathophysiology
- Protein purification and stability
- SARS-CoV-2 and COVID-19 Research
- Injection Molding Process and Properties
- Neonatal Health and Biochemistry
University of Trento
2021
SARS-CoV-2 spike N-terminal domain harbors a potent epitope that can be modulated by binding of natural linear tetrapyrroles.
The coronaviral spike is the dominant viral antigen and target of neutralizing antibodies. We show that SARS-CoV-2 binds biliverdin bilirubin, tetrapyrrole products haem metabolism, with nanomolar affinity. Using cryo-electron microscopy X-ray crystallography we mapped interaction pocket to a deep cleft on N-terminal domain (NTD). At physiological concentrations, significantly dampened reactivity immune sera inhibited subset Access tetrapyrrole-sensitive epitope gated by flexible loop distal...
The anti-HIV drug efavirenz (EFV) displays low and variable bioavailability because of its poor aqueous solubility. Ball milling is a simple cost-effective alternative to traditional micronization improve the solubility dissolution rate EFV. A multibody dynamics model was employed optimize process parameters, while motion balls in mill jar monitored operando. This led better understanding for efficient comminution enhancement EFV dissolution. variability results different batches also...