A5089340042- Bacterial Genetics and Biotechnology
- Biochemical and Molecular Research
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Antifungal resistance and susceptibility
- Meat and Animal Product Quality
- Cancer, Hypoxia, and Metabolism
- GABA and Rice Research
- Metabolism and Genetic Disorders
- Escherichia coli research studies
- Probiotics and Fermented Foods
- Catalysis for Biomass Conversion
- Phytochemicals and Antioxidant Activities
- Oral microbiology and periodontitis research
- Amino Acid Enzymes and Metabolism
- Microbial Fuel Cells and Bioremediation
- Salmonella and Campylobacter epidemiology
- Metalloenzymes and iron-sulfur proteins
- Microbial Metabolism and Applications
- Bacterial biofilms and quorum sensing
- Food Quality and Safety Studies
University of Wisconsin–Madison
2014-2025
Maejo University
2023-2024
Bowdoin College
2011-2018
Great Lakes Bioenergy Research Center
2015-2016
Genetic diversity is often generated during adaptation to stress, and in eukaryotes some of this thought arise via recombination reassortment alleles meiosis. Candida albicans, the most prevalent pathogen humans, has no known meiotic cycle, yet it a heterozygous diploid that undergoes mitotic somatic growth. It been shown clinical isolates as well strains passaged once through mammalian host undergo increased levels recombination. Here, we tested hypothesis stress conditions increase rates...
Bacterial biofilms are ubiquitous in natural environments and play an important role many clinical, industrial, ecological settings. Although much is known about the transcriptional regulatory networks that control biofilm formation model bacteria such as Bacillus subtilis , very little of metabolism this complex developmental process. To address knowledge gap, we performed a time-resolved analysis metabolic changes associated with bacterial development B. by combining metabolomic,...
In vitro studies suggest that stress may generate random standing variation and different cellular ploidy states evolve more rapidly under stress. Yet this idea has not been tested with pathogenic fungi growing within their host niche in vivo Here, we analyzed the generation of both genotypic phenotypic diversity during exposure Candida albicans to mouse oral cavity. Ploidy, aneuploidy, loss heterozygosity (LOH), recombination were determined using flow cytometry double digest restriction...
ABSTRACT An outstanding challenge toward efficient production of biofuels and value-added chemicals from plant biomass is the impact that lignocellulose-derived inhibitors have on microbial fermentations. Elucidating mechanisms underlie their toxicity critical for developing strategies to overcome them. Here, using Escherichia coli as a model system, we investigated metabolic effects feruloyl amide coumaroyl amide, predominant phenolic compounds in ammonia-pretreated hydrolysates. Using...
ABSTRACT Guanosine triphosphate (GTP) is essential for macromolecular biosynthesis, and its intracellular levels are tightly regulated in bacteria. Loss of the alarmone (p)ppGpp disrupts GTP regulation Bacillus subtilis , causing cell death presence exogenous guanosine underscoring critical importance homeostasis. To investigate basis toxicity, we performed a genetic selection spontaneous mutations that suppress this effect, uncovering an unexpected link between synthesis glycolysis. In...
Salmonella enterica is a member of the plant microbiome. Growth S. in sprouting-seed exudates rapid; however, active metabolic networks essential this environment are unknown. To examine requirements during growth exudates, we inoculated alfalfa seeds and identified 305 proteins extracted 24 h postinoculation from planktonic cells. Over half had known functions, they involved over one-quarter reactions. Ion metabolite transport accounted for majority detected Proteins amino acid metabolism...
Background: Global awareness of ambient air pollution has heightened due to its detrimental impact on health, particularly in regions with elevated PM2.5 levels. Chiang Mai emerged as an area experiencing the highest levels Thailand. Objectives: examine prevalence respiratory allergies and assess health-related quality life (QoL) among university students Mai. Methods: University (CMU) Maejo (MJU) were recruited. The Asthma Network (GAN) questionnaire screened for (RAs). disease-specific QoL...
Functional fermented fruit juices produced using a combination of non-Saccharomyces yeast and lactic acid bacteria (LAB) are relatively unexplored. The effects three inoculation protocols, single with Lactobacillus acidophilus (La), Torulaspora delbrueckii (Td), co-culture both La + Td, on the physicochemical, microbiological, sensory properties, metabolic profile JA after 24 h at 30 °C were investigated. Uninoculated (UI) Japanese apricot (JA) juice was used as control. results show...
NADH:quinone oxidoreductase (complex I) is a bioenergetic enzyme that transfers electrons from NADH to quinone, conserving the energy of this reaction by contributing proton motive force. While importance oxidation mitochondrial aerobic respiration well documented, contribution complex I bacterial electron transport chains has been tested in only few species. Here, we analyze function two phylogenetically distinct isozymes Rhodobacter sphaeroides, an alphaproteobacterium contains...
The human enteric pathogen Salmonella enterica leads a cross-kingdom lifestyle, actively colonizing and persisting on plants in between animal hosts. One of the questions that arises from this dual lifestyle is how S. able to adapt such divergent Metabolic pathways required for colonization virulence have been previously identified, but metabolism bacterium poorly understood. To determine metabolic requirements plant by enterica, we first screened library mutants, examined systemic mouse...
Guanosine triphosphate (GTP) is essential for macromolecular biosynthesis, and its intracellular levels are tightly regulated in bacteria. Loss of the alarmone (p)ppGpp disrupts GTP regulation Bacillus subtilis , causing cell death presence exogenous guanosine underscoring critical importance homeostasis. To investigate basis toxicity, we performed a genetic selection spontaneous mutations that suppress this effect, uncovering an unexpected link between synthesis glycolysis. In particular,...
Importance of the work: Exploring physicochemical and biological properties probiotic bacteria provides insights into quality honey from Chiang Mai, one Thailand's important honey-producing sites.Objectives: To explore potential local raw produced in Mai province. Materials & Methods:The were investigated four nonprocessed stingless bee (SH) honeybee (HH) samples locally collected several districts province, Thailand.Assessment consisted high-performance liquid chromatography...