A5082207112- Environmental DNA in Biodiversity Studies
- Identification and Quantification in Food
- Species Distribution and Climate Change
- Microbial Community Ecology and Physiology
- Wildlife Ecology and Conservation
- Forest Insect Ecology and Management
- Mycorrhizal Fungi and Plant Interactions
- Forest Ecology and Biodiversity Studies
- Genomics and Phylogenetic Studies
- Lichen and fungal ecology
- Plant Pathogens and Fungal Diseases
- Human-Animal Interaction Studies
- Protist diversity and phylogeny
- Primate Behavior and Ecology
- Insect-Plant Interactions and Control
- Smart Agriculture and AI
- Genetic diversity and population structure
- Remote Sensing in Agriculture
- Remote Sensing and LiDAR Applications
- Genetic and phenotypic traits in livestock
- Indoor Air Quality and Microbial Exposure
- Ecology and Vegetation Dynamics Studies
- Insect and Arachnid Ecology and Behavior
- Wildlife-Road Interactions and Conservation
Kunming Institute of Zoology
2012-2024
Chinese Academy of Sciences
2012-2024
Summary 1. Traditional biodiversity assessment is costly in time, money and taxonomic expertise. Moreover, data are frequently collected ways (e.g. visual bird lists) that unsuitable for auditing by neutral parties, which necessary dispute resolution. 2. We present protocols the extraction of ecological, phylogenetic information from bulk samples arthropods. The combine mass trapping arthropods, mass‐PCR amplification COI barcode gene, pyrosequencing bioinformatic analysis, together we call...
The targeted sequencing of taxonomically informative genetic markers, sometimes known as metabarcoding, allows eukaryote biodiversity to be measured rapidly, cheaply, comprehensively, repeatedly, and verifiably. Metabarcoding helps remove the taxonomic impediment, which refers great logistical difficulties describing identifying species, thus promises improve our ability detect respond changes in natural environment. Now, sampling has become a rate-limiting step measurement, an effort reduce...
Abstract Pacific salmon are a keystone resource in Alaska, generating annual revenues of well over ~US$500 million/year. Due to their anadromous life history, adult spawners distribute amongst thousands streams, posing huge management challenge. Currently, enumerated at just few streams because reliance on human counters and, rarely, sonar. The ability detect organisms by shed tissue (environmental DNA, eDNA) promises more efficient counting method. However, although eDNA correlates...
Abstract Despite widespread recognition of its great promise to aid decision‐making in environmental management, the applied use metabarcoding requires improvements reduce multiple errors that arise during PCR amplification, sequencing and library generation. We present a co‐designed wet‐lab bioinformatic workflow for bulk samples removes both false‐positive (tag jumps, chimeras, erroneous sequences) false‐negative (‘dropout’) errors. However, we find it is not possible recover...
Abstract Recently, detection dogs have been utilized to collect fecal samples from cryptic and rare mammals. Despite the great promise of this technique for conservation biology, its broader application has limited by high cost (tens hundreds thousands dollars) logistical challenges employing a scat-detection dog team while conducting international, collaborative research. Through an international collaboration primatologists Chinese Ministry Public Security, we trained used find scat three...
Abstract Protected areas are key to meeting biodiversity conservation goals, but direct measures of effectiveness have proven difficult obtain. We address this challenge by using environmental DNA from leech-ingested bloodmeals estimate spatially-resolved vertebrate occupancies across the 677 km 2 Ailaoshan reserve in Yunnan, China. From 30,468 leeches collected 163 park rangers 172 patrol areas, we identify 86 species, including amphibians, mammals, birds and squamates. Multi-species...
The Honghe-Hani landscape in China is a UNESCO World Natural Heritage site due to the beauty of its thousands rice terraces, but these structures are danger from invasive crayfish Procambarus clarkii. Crayfish dig nest holes, which collapse terrace walls and destroy production. Under current control strategy, farmers self-report issued pesticide, this strategy not expected eradicate nor prevent their spread since able detect small numbers crayfish. Thus, we tested whether environmental DNA...
Wood decomposition releases almost as much CO2 to the atmosphere does fossil-fuel combustion, so factors regulating wood can affect global carbon cycling. We used metabarcoding estimate fungal species diversities of naturally colonized decomposing in subtropical China and, for first time, compared them concurrent measures emissions. hosting more diverse communities emitted less CO2, with Shannon diversity explaining 26 44% emissions variation. Community analysis supports a 'pure diversity'...
The Tibetan fox (Vulpes ferrilata) is generally acknowledged to be a specialist forager on its preferred prey, the burrowing lagomorph plateau pika (Ochotona curzoniae), but whether true dependency characterizes relationship remains unclear. We estimated presence of foxes in 62 habitat patches that reflected continuum environmental conditions within their known geographic distribution Qinghai Province, China. used site-occupancy modeling and quantified abundance pikas as well other site...
1 Abstract Protected areas are central to meeting biodiversity conservation goals, but measuring their effectiveness is challenging. We address this challenge by using DNA from leech-ingested bloodmeals estimate vertebrate occupancies across the 677 km 2 Ailaoshan reserve in Yunnan, China. 163 park rangers collected 30,468 leeches 172 patrol areas. identified 86 species, including amphibians, mammals, birds, and squamates. Multi-species occupancy modelling showed that species richness...
Abstract Despite widespread recognition of its great promise to aid decision-making in environmental management, the applied use metabarcoding requires improvements reduce multiple errors that arise during PCR amplification, sequencing, and library generation. We present a co-designed wet-lab bioinformatic workflow for bulk samples removes both false-positive (tag jumps, chimeras, erroneous sequences) false-negative (‘dropout’) errors. However, we find it is not possible recover...
Abstract Despite decades of field study, very little is known about the molecular ecology gibbons, particularly as it relates to their ability disperse across degraded and fragmentary landscapes. The critically endangered western black crested gibbon ( Nomascus concolor ) has been reduced a small, fragmented population with 1300 individuals. In largest genetic study free‐ranging gibbons date, we sampled 47 these from 13 sites in China generated 15 polymorphic autosomal microsatellite...
Abstract The Honghe-Hani landscape in China is a UNESCO World Natural Heritage site due to the beauty of its thousands rice terraces, but these structures are danger from invasive crayfish Procambarus clarkii . Crayfish dig nest holes, which collapse terrace walls and destroy production. Under current control strategy, farmers self-report issued pesticide, this strategy not expected eradicate nor prevent their spread since able detect small numbers crayfish. Thus, we tested whether...
Abstract Pacific salmon are a keystone resource in Alaska, generating annual revenues of well over ∼US$500 million/yr. Due to their anadromous life history, adult spawners distribute amongst thousands streams, posing huge management challenge. Currently, enumerated at just few streams because reliance on human counters and, rarely, sonar. The ability detect organisms by shed tissue (environmental DNA, eDNA) promises more efficient counting method. However, although eDNA correlates generally...
Abstract Plant diversity surely determines arthropod diversity, but only moderate correlations between and plant species richness had been observed until Basset et al. (2012, Science 338: 1481-1484) finally undertook an unprecedentedly comprehensive sampling of a tropical forest demonstrated that could indeed accurately predict richness. We now require high-throughput pipeline to operationalize this result so we can (1) test competing explanations for megadiversity, (2) improve estimates...
Abstract Wood decomposition releases almost as much CO 2 to the atmosphere does fossil-fuel combustion, so factors regulating wood can affect global carbon cycling. We used metabarcoding estimate fungal species diversities of naturally colonized decomposing in subtropical China and, for first time, compared them concurrent measures emissions. hosting more diverse communities emitted less , with Shannon diversity explaining 26 44% emissions variation. Community analysis supports a ‘pure...