A5033338494- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Amino Acid Enzymes and Metabolism
- Gastrointestinal motility and disorders
- Innovative Microfluidic and Catalytic Techniques Innovation
- Membrane-based Ion Separation Techniques
- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- Gene Regulatory Network Analysis
- 3D Printing in Biomedical Research
- RNA and protein synthesis mechanisms
- Neurobiology and Insect Physiology Research
- Gut microbiota and health
- Alkaloids: synthesis and pharmacology
- Supercapacitor Materials and Fabrication
- Microbial Fuel Cells and Bioremediation
University of Washington
2023-2025
Georgia Institute of Technology
2019-2021
Engineered living materials (ELMs) fabricated by encapsulating microbes in hydrogels have great potential as bioreactors for sustained bioproduction. While long-term metabolic activity has been demonstrated these systems, the capacity and dynamics of gene expression over time is not well understood. Thus, we investigate microbial ELMs constructed using different hydrogel matrices. Through direct measurements engineered E. coli F127-bisurethane methacrylate (F127-BUM) hydrogels, show that...
Membrane proteins are present in a wide array of cellular processes from primary and secondary metabolite synthesis to electron transport single carbon metabolism. A key barrier applying membrane industrially is their difficult functional production. Beyond expression, folding, insertion, protein activity influenced by the physicochemical properties associated membrane, making it achieve optimal performance outside endogenous host. In this review, we highlight recent work on production...
Olfactory receptors are ectopically expressed (exORs) in more than 16 different tissues. Studying the role of exORs is hindered by lack known ligands that activate these receptors. Of particular interest colon, section gastrointestinal tract with greatest diversity microbiota where ORs may be participating host–microbiome communication. Here, we leverage a G-protein-coupled receptor (GPCR)-based yeast sensor strain to generate sensors for seven highly colon. We screen colon against 57...
Formate, a biologically accessible form of CO2, has attracted interest as renewable feedstock for bioproduction. However, approaches are needed to investigate efficient routes biological formate assimilation due its toxicity and limited utilization by microorganisms. Cell-free systems hold promise their potential use carbon energy sources compatibility with diverse feedstocks. bioproduction using purified cell-free is costly enzyme purification, whereas lysate-based must overcome loss flux...
The serotonin receptor 4b (5-HTR4b) is expressed throughout the gastrointestinal tract, and its agonists are used in treatment of irritable bowel syndrome with constipation (IBS-C). Today, there no rapid assays for identification 5-HTR4b agonists. Here, we developed a luciferase-based assay capable assessing one compound per second 38-fold dynamic range nM limit detection serotonin. We to screen more than 1000 natural products anti-infection agents identified five new ligands: hordenine,...
Formate, a biologically accessible form of CO2, has attracted interest as renewable feedstock for bioproduction. However, its use in microorganisms suffers from toxicity and limited utilization. Approaches are needed to investigate efficient routes biological formate assimilation. Cell-free systems hold promise due their potential carbon energy sources compatibility with diverse feedstocks. bioproduction using purified cell-free is by costly enzyme purification, whereas lysate-based must...