A5048780355- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Neuroscience of respiration and sleep
- Neuroendocrine regulation and behavior
- Pain Mechanisms and Treatments
- Cardiac electrophysiology and arrhythmias
- Stress Responses and Cortisol
- Nicotinic Acetylcholine Receptors Study
- Heart Rate Variability and Autonomic Control
- Neuropeptides and Animal Physiology
- Neuroscience and Neural Engineering
- Antimicrobial Peptides and Activities
- Hypothalamic control of reproductive hormones
- Neuroinflammation and Neurodegeneration Mechanisms
- Helminth infection and control
- MicroRNA in disease regulation
- Acupuncture Treatment Research Studies
- Neural dynamics and brain function
- Lipid Membrane Structure and Behavior
- Barrier Structure and Function Studies
- Sleep and Wakefulness Research
- Erythropoietin and Anemia Treatment
- Neurobiology and Insect Physiology Research
- Adipose Tissue and Metabolism
- Neurogenesis and neuroplasticity mechanisms
Seoul National University
2015-2024
Chonnam National University
2014
Korea Institute of Oriental Medicine
2011
Korea Advanced Institute of Science and Technology
2011
Soongsil University
2008
Yonsei University
2008
Heifer International
2007
Korea National Institute of Health
2005
Chungnam National University
2003
Konkuk University
2000
Abstract: Gold nanoparticles (AuNPs) are attractive materials for use in biomedicine due to their physical properties. Increasing evidence suggests that several induce the differentiation of human mesenchymal stem cells into osteoblasts and adipocytes. In this study, we hypothesized chitosan-conjugated AuNPs promote osteogenic adipose-derived cells. For evaluation differentiation, alizarin red staining, an alamarBlue ® assay, a quantitative real-time polymerase chain reaction analysis were...
A 20-residue hybrid peptide CA(1−8)−MA(1−12) (CA−MA), incorporating residues 1−8 of cecropin (CA) and 1−12 magainin 2 (MA), has potent antimicrobial activity without toxicity against human erythrocytes. To investigate the effects Gly-Ile-Gly hinge sequence CA−MA on antibacterial antitumor activities, two analogues in which is either deleted (P1) or substituted with Pro (P2) were synthesized. The role tryptophan residue at position its antibiotic was also investigated using analogues, Trp2...
1. In freshly isolated spinal dorsal horn (DH) neurons (laminae I-III) of the young rat, effects tachykinins (substance P, neurokinin A) on inward current induced by excitatory amino acids were studied under whole-cell voltage-clamp conditions. 2. When cells clamped to a holding potential -60 mV, simultaneous application N-methyl-D-aspartate (NMDA) (10(-4) M) and substance P (SP) (2 x 10(-9)-10(-7) for 10 s reversibly enhanced (by 129.6 +/- 8.2%, mean SE) peak amplitude initial transient...
1. Calcium currents in immature rat spinal dorsal horn neurons transverse slices were studied with the single-electrode voltage-clamp technique. Using experimental conditions that minimized voltage-dependent Na+ and K+ currents, we distinguished low- high-voltage-activated calcium on basis of their voltage dependence sensitivity to Ca2(+)-channel agonist antagonist drugs. 2. The low-voltage-activated transient current is evoked weak depolarizing commands. It begins activate at potentials...
Niemann Pick disease type C1 (NPC) is an autosomal recessive characterized by progressive neurological deterioration leading to premature death. In this study, we hypothesized that human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have the multifunctional abilities ameliorate NPC symptoms in brain. To test hypothesis, hUCB-MSCs were transplanted into hippocampus of mice early asymptomatic stage. This transplantation resulted recovery motor function Rota Rod and impaired...
Noradrenaline (NA) is a major neurotransmitter that regulates many neuroendocrine and sympathetic autonomic functions of the hypothalamic paraventricular nucleus (PVN). Previously NA has been shown to increase frequency excitatory synaptic activity parvocellular neurons within PVN, but little known about its effects on inhibitory activity. In this work, we studied (1–100 μM) spontaneous currents (sIPSC) type II PVN in brain slices rat using whole cell patch-clamp technique. Spontaneous IPSCs...
1. The membrane actions of substance P (SP) and a related tachykinin, neurokinin A (NKA), have been investigated by means single-electrode, voltage-clamp technique in the immature rat dorsal horn neurons using an vitro spinal cord slice preparation. 2. When potential was held at resting level between -75 -55 mV, bath application SP or NKA (10(-7) to 10(-5) M, for 1-3 min) induced inward shift holding current lasting several minutes. magnitude this effect varied 10 400 pA depending on...
Voltage-gated <TEX>$K^+$</TEX> (Kv) channels are widely expressed in the plasma membranes of numerous cells such as epithelial cells. Recently, it has been demonstrated that Kv associated with proliferation several types cancer Specifically, Kv1.3 seems to be involved cell and apoptosis. In present study, we examined expression immortalized tumorigenic human mammary We also evaluated level each stage breast using mRNA isolated from patients. addition, treatment tetraethylammonium, a channel...
Detection of single point mutation based on the hybridization oligonucleotides was performed using unmodified gold nanoparticles. The sequences were designed to detect metastatic efficiency modifier signal-induced proliferation-associated gene 1 (Sipa1). detection step monitored UV−vis absorption spectroscopy, quasielastic light scattering, and zeta potential measurement. We observed that addition DNAs into suspension nanoparticles could substantially aggregate change color solution. By...
Objectives The in vivo efficacy of a cefotaxime-ciprofloxacin combination against Vibrio vulnificus and the effects on rtxA1 expression commonly used antibiotics are unknown. Methods In vitro time-kill studies were performed to evaluate synergism. Female BALB/c mice injected subcutaneously with 1×107 or 1×108 cfu V. vulnificus. Antibiotic therapy was initiated at 2 h after inoculation following four groups: cefotaxime; ciprofloxacin; cefotaxime-plus-ciprofloxacin;...
Stem cell-based therapy is one of the most attractive approaches to ischemic heart diseases, such as myocardial infarction (MI). We evaluated cardio-protective effects human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) stably expressing lymphoid enhancer-binding factor 1 (LEF1; LEF1/hUCB-MSCs) in a rat model MI. LEF1 overexpression hUCB-MSCs promoted cell-proliferation and anti-apoptotic hypoxic conditions. For application its therapeutic vivo, gene was introduced into an...
The baroreceptor reflex is a powerful neural feedback that regulates arterial pressure (AP). Mechanosensitive channels transduce pulsatile AP to electrical signals in baroreceptors. Here we show tentonin 3 (TTN3/TMEM150C), cation channel activated by mechanical strokes, essential for detecting changes the aortic arch. TTN3 was expressed nerve terminals arch and nodose ganglion (NG) neurons. Genetic ablation of Ttn3 induced ambient hypertension, tachycardia, fluctuations, impaired baroreflex...
Neuronal activity in the paraventricular nucleus (PVN) is known to be elevated rats with heart failure. However, type of neurons involved and underlying synaptic mechanisms remain unknown. Here we examined spontaneous firing currents presympathetic PVN myocardial infarction (MI), using slice patch clamp combined retrograde labeling technique. In projecting rostral ventrolateral medulla (PVN-RVLM), MI induced a significant increase basal rate (1.79 3.02 Hz, P < 0.05) reduction frequency (P...
Abstract It is well established that the GABAA receptor plays an important role in regulating electrical excitability of GnRH neurons. Two different modes signaling exist: one mediated by synaptic receptors generating fast (phasic) postsynaptic currents and other extrasynaptic a persistent (tonic) current. Using antagonists picrotoxin, bicuculline methiodide, gabazine, which differentiate between phasic tonic signaling, we found ∼50% neurons exhibit approximately 15-pA current acute brain...
Despite the well-established contribution of neurohumoral activation to morbidity and mortality in heart failure (HF) patients, relatively little is known about underlying central nervous system mechanisms. In this study, we aimed determine whether changes GABAergic inhibitory glutamatergic excitatory synaptic function contribute altered hypothalamic magnocellular neurosecretory cell (MNC) activity HF rats. Patch-clamp recordings were obtained from MNCs brain slices sham Glutamate (EPSCs)...