A5049908780- Extracellular vesicles in disease
- Lipid Membrane Structure and Behavior
- Listeria monocytogenes in Food Safety
- Nanopore and Nanochannel Transport Studies
- Biosensors and Analytical Detection
- 3D Printing in Biomedical Research
- Signaling Pathways in Disease
- MicroRNA in disease regulation
- Algal biology and biofuel production
- Advanced biosensing and bioanalysis techniques
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Bacteriophages and microbial interactions
- Erythrocyte Function and Pathophysiology
- Micro and Nano Robotics
- Periodontal Regeneration and Treatments
- Enzyme Catalysis and Immobilization
- Plant and Fungal Interactions Research
- Air Quality and Health Impacts
- Essential Oils and Antimicrobial Activity
- Toxoplasma gondii Research Studies
- Nanoparticles: synthesis and applications
- Chemical Synthesis and Analysis
- Acoustic Wave Resonator Technologies
- Characterization and Applications of Magnetic Nanoparticles
National Institute of Chemistry
2015-2025
University of Ljubljana
2024
Google (United States)
2017
Abstract The invertebrate cytolysin lysenin is a member of the aerolysin family pore-forming toxins that includes many representatives from pathogenic bacteria. Here we report crystal structure pore and provide insights into its assembly mechanism. assembled nine monomers via dramatic reorganization almost half monomeric subunit leading to β-barrel ∼10 nm long 1.6–2.5 wide. devoid additional luminal compartments as commonly found in other toxin pores. Mutagenic analysis atomic force...
Abstract Pore formation of cellular membranes is an ancient mechanism bacterial pathogenesis that allows efficient damaging target cells. Several mechanisms have been described, however, relatively little known about the assembly and properties pores. Listeriolysin O (LLO) a pH-regulated cholesterol-dependent cytolysin from intracellular pathogen Listeria monocytogenes , which forms transmembrane β-barrel Here we report LLO pores rapid irrespective pH. While pore diameters at membrane...
Abstract Background A major bottleneck in drug delivery is the breakdown and degradation of system through endosomal/lysosomal network host cell, hampering correct interest. In nature, bacterial pathogen Listeria monocytogenes has developed a strategy to secrete Listeriolysin O (LLO) toxin as tool escape eukaryotic lysosomal upon infection, allowing it grow proliferate unharmed inside cell. Results As “proof concept”, we present here use purified His-LLO H311A mutant protein its conjugation...
Unprotected dipeptides are attractive building blocks for environmentally friendly hydrogel biomaterials by virtue of their low-cost and ease preparation. This work investigates the self-assembling behaviour distinct stereoisomers Ile-Phe Phe-Ile in phosphate buffered saline (PBS) to form hydrogels, using transmission electron microscopy (TEM), attenuated total reflectance infrared spectroscopy (ATR-IR), circular dichroism (CD), oscillatory rheometry. Each peptide purity identity was also...
Abstract Listeriolysin O (LLO) is a cytolysin capable of forming pores in cholesterol-rich lipid membranes host cells. It conveniently suited for engineering pH-governed responsiveness, due to pH sensor identified its structure that was shown before affect stability. Here we introduced new level control hemolytic activity by making variant with pH-dependent. Based on detailed structural analysis coupled molecular dynamics and mutational analysis, found the bulky side chain Tyr406...
Despite many advances in gene therapy, the delivery of small interfering RNAs is still challenging. Erythrocytes are most abundant cells human body, and their membrane possesses unique features. From them, erythrocytes vesicles can be generated, employable as nano drug system with prolonged blood residence high biocompatibility. Human erythrocyte ghosts were extruded presence siRNA, objects termed EMVs (erythrocyte vesicles). An ultracentrifugation-based method was applied to select only...
Extracellular vesicles (EVs) are gaining increasing amounts of attention due to their potential use in diagnostics and therapy, but the poor reproducibility studies that have been conducted on these structures hinders breakthrough into routine practice. We believe a better understanding EVs stability methods control integrity key resolving this issue. In work, erythrocyte (hbEVs) were isolated by centrifugation from suspensions human erythrocytes had aged vitro. The isolate was characterised...
Tumor growth and metastasis strongly rely on cell-cell communication. One of the mechanisms by which tumor cells communicate involves release uptake lipid membrane encapsulated particles full bioactive molecules, called extracellular vesicles (EVs). EV exchange between cancer may induce phenotype changes in recipient cells. Our work investigated effect EVs released teratocarcinoma glioblastoma (GBM) were isolated differential centrifugation analyzed through Western blot, nanoparticle...
Listeria monocytogenes is a food and soil-borne pathogen that secretes pore-forming toxin listeriolysin O (LLO) as its major virulence factor. We tested the effects of LLO on an intestinal epithelial cell line Caco-2 compared them to unrelated equinatoxin II (EqtII). Results showed apical application both toxins causes significant drop in transepithelial electrical resistance (TEER), with higher concentrations or prolonged exposure time needed achieve same magnitude response than EqtII. The...
Plant-derived nanovesicles (NVs) have attracted interest due to their anti-inflammatory, anticancer and antioxidative properties efficient uptake by human intestinal epithelial cells. Previously we showed that tomato (Solanum lycopersicum L.) fruit is one of the interesting plant resources from which NVs can be obtained at a high yield. In course isolation different batches tomatoes, using established differential ultracentrifugation or size-exclusion chromatography methods, occasionally...
Small particles in natural sources are a subject of interest for their potential role intercellular, inter-organism, and inter-species interactions, but harvesting assessment present challenge due to small size transient identity. We applied recently developed interferometric light microscopy (ILM) assess the number density hydrodynamic radius (Rh) isolated cellular (SCPs) from blood preparations (plasma washed erythrocytes) (B), spruce needle homogenate (S), suspension flagellae microalgae...
We studied the efficiency of three culture series microalgae Phaeodactylum tricornutum (P. tricornutum) and bacteria Thalassospira sp. (axenic microalgae, bacterial co-culture two) in removing bisphenols (BPs) from their growth medium. Bacteria were identified by 16S ribosomal RNA polymerase chain reaction (16S rRNA PCR). The microorganism rate was determined flow cytometry. Cultures isolates small cellular particles (SCPs) imaged scanning electron microscopy (SEM) cryogenic transmission...
Lipid nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called nano-sized hybridosomes or nanohybridosomes, NSHs) were considered.
Lipid membranes are becoming increasingly popular in synthetic biology due to their biophysical properties and crucial role communication between different compartments. Several alluring protein–membrane sensors have already been developed, whereas protein logic gates designs on membrane-embedded proteins very limited. Here we demonstrate the construction of a two-level gate with an OR-AND logic. The system consists engineered pH-dependent pore-forming listeriolysin O its DARPin-based...
Small cellular particles (SCPs) are being considered for their role in cell-to-cell communication. We harvested and characterized SCPs from spruce needle homogenate. were isolated by differential ultracentrifugation. They imaged scanning electron microscope (SEM) cryogenic transmission (cryo TEM), assessed number density hydrodynamic diameter interferometric light microscopy (ILM) flow cytometry (FCM), total phenolic content (TPC) UV-vis spectroscopy, terpene gas chromatography-mass...
<ns3:p><ns3:bold>Background:</ns3:bold> Cells in general secrete nanoparticles (NPs) which are believed to mediate intercellular communication. Recently, great efforts have been made utilize them as delivery vectors. We aimed harvest and identify NPs from liquid cultures of two marine microalgae <ns3:italic>Dunaliella tertiolecta </ns3:italic>and<ns3:italic> Phaeodactyum tricornutum</ns3:italic>.</ns3:p><ns3:p> <ns3:bold>Methods: </ns3:bold>NPs were isolated the culture conditioned media by...
A major drawback of the IgG capture step is high cost protein resin. For a better utilization resin, continuous multi-column operation was recently proposed. In this method, accurate detection leaking crucial to divert breakthrough fluid from waste next column and prolong loading without product loss. The point as change in UV absorption based on relatively small signal addition IgGs bulk host cell proteins. To achieve specificity, we used quartz crystal microbalance immobilized specific...
Abstract Cryogenic transmission electron images of small cellular particles isolated from conditioned media microalgae Dunaliellatertiolecta are presented Each image is supplemented by description the preparation sample and data on imaging technique equipment. Keywords: Extracellular vesicles, particles, Nanoalgosomes, Exosomes
Abstract: Cryogenic transmission electron images of small cellular particles isolated from conditioned media microalgae Phaeodactylum tricornutun are presented . Each image is identified by the name and number that constitutes title Figure, supplemented description preparation sample data on imaging technique equipment. Keywords: Extracellular vesicles, particles, Nanoalgosomes, Exosomes
The crucial molecular factors that shape the interfaces of lipid-binding proteins with their target ligands and surfaces remain unknown due to complex makeup biological membranes. Cholesterol, major modulator bilayer structure in mammalian cell membranes, is recognized by various proteins, including well-studied cholesterol-dependent cytolysins. Here, we use vitro evolution investigate adaptations preserve cholesterol specificity perfringolysin O, prototypical cytolysin from Clostridium...
Extracellular nanoparticles (EPs) are a subject of increasing interest for their biological role as mediators in cell–cell communication; however, harvesting and assessment from bodily fluids challenging, processing can significantly affect samples. With the aim minimizing artifacts, we assessed number density (n) hydrodynamic diameter (Dh) EPs directly diluted plasma blood using following recently developed technique: interferometric light microscopy (ILM). We analyzed 613 samples human...