A5031328407- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neural Engineering
- Ion channel regulation and function
- Obstructive Sleep Apnea Research
- Neural dynamics and brain function
- Cardiac Arrhythmias and Treatments
- Neuroscience of respiration and sleep
- Atrial Fibrillation Management and Outcomes
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Nicotinic Acetylcholine Receptors Study
- stochastic dynamics and bifurcation
- Receptor Mechanisms and Signaling
- Pluripotent Stem Cells Research
- Cardiomyopathy and Myosin Studies
- Signaling Pathways in Disease
- Electrochemical Analysis and Applications
- Cardiac Fibrosis and Remodeling
- Cardiovascular and Diving-Related Complications
- Cardiac Ischemia and Reperfusion
- ECG Monitoring and Analysis
- Semiconductor materials and devices
- Mesenchymal stem cell research
- Statistical Methods in Clinical Trials
- Protein Kinase Regulation and GTPase Signaling
University at Buffalo, State University of New York
2018-2024
Masonic Medical Research Laboratory
2013-2019
Jacobs Institute
2019
York University
2016
University of Toronto
2010-2013
Heart and Stroke Foundation
2011-2013
Universität Innsbruck
2011
University of Michigan
2005-2011
St. Boniface Hospital
2011
University Health Network
2011
It has long been recognized that cationic nanoparticles induce cell membrane permeability. Recently, it found the formation and/or growth of nanoscale holes in supported lipid bilayers. In this paper, we show noncytotoxic concentrations 30−2000 pA currents 293A (human embryonic kidney) and KB epidermoid carcinoma) cells, consistent with a defect such as single hole or group ranging from 1 to 350 nm2 total area. Other forms defects, including nanoparticle porating agents adsorbing onto...
Chronic iron overload (CIO) is associated with blood disorders such as thalassemias and hemochromatosis. A major prognostic indicator of survival in patients CIO iron-mediated cardiomyopathy characterized by contractile dysfunction electrical disturbances, including slow heart rate (bradycardia) block.We used a mouse model to investigate the effects on sinoatrial node (SAN) function. As humans, reduced (≈20%) conscious mice well anesthetized autonomic nervous system blockade isolated...
The fast transient outward K(+) current (I(to,f)) plays a critical role in early repolarization of the heart. I(to,f) is consistently downregulated cardiac disease. Despite its importance, regulation disease remains poorly understood.Because transcription factor nuclear (NF)-κB activated hypertrophy and disease, we studied NF-κB mediating reductions induced by hypertrophy.Culturing neonatal rat ventricular myocytes presence phenylephrine (PE) plus propranolol (Pro), to selectively activate...
Abstract Obstructive sleep apnoea (OSA) affects 9–24% of the adult population. OSA is associated with atrial disease, including enlargement, fibrosis and arrhythmias. Despite link between cardiac molecular changes in heart which occur remain elusive. To study OSA‐induced changes, we utilized a recently developed rat model closely recapitulates characteristics OSA. Male Sprague Dawley rats, aged 50–70 days, received surgically implanted tracheal balloons were inflated to cause transient...
Brugada syndrome (BrS) is an inherited cardiac arrhythmia commonly associated with SCN5A mutations, yet its ionic mechanisms remain unclear due to a lack of cellular models. Here, we used human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) from BrS patient (BrS1) evaluate the roles Na+ currents (INa) and transient outward K+ (Ito) in action potential (AP) changes. To understand role these current changes repolarization employed dynamic clamp "electronically express" IK1...
Recent studies have shown that Kir2 channels display differential sensitivity to intracellular polyamines, and raised a number of questions about several properties inward rectification important the understanding their physiological roles. In this study, we carried out detailed characterization steady‐state kinetic block Kir2.1–3 by spermine. High‐resolution recordings from outside‐out patches showed in all current–voltage relationships ‘crossover’ effect upon change extracellular K + ....
Previous studies have shown that cardiac inward rectifier potassium current ( I K1 ) channels are heteromers of distinct Kir2 subunits and suggested species- tissue-dependent expression these may underlie variability . In this study, we investigated the contribution slowly activating Kir2.3 subunit free intracellular polyamines (PAs) to in mouse heart. The kinetics activation was measured concatemeric tetramers with known stoichiometry. Inclusion only one a Kir2.1 channel led an approximate...
Kir2 subunits form channels that underlie classical strongly inwardly rectifying potassium currents. While homomeric display a number of distinct and physiologically important properties, the functional properties heteromeric assemblies, as well stoichiometries arrangements in native channels, remain largely unknown. Therefore, we have implemented concatemeric approach, whereby all four cloned were linked tandem, order to study effects Kir2.1 Kir2.2 heteromerization on resulting channels....
Transplantation of mesenchymal stem cells (MSCs) has shown therapeutic potential for cardiovascular diseases, but the electrophysiological implications are not understood. The purpose this study was to evaluate impact MSC transplantation on adverse remodeling in heart following myocardial infarction (MI).Three weeks after coronary ligation induce MI rats, MSCs or culture medium were directly injected into each infarct. One two later, hearts excised, Langendorff-perfused, and optically mapped...
Abstract Obstructive sleep apnea (OSA) affects an estimated 20% of adults worldwide and has been associated with electrical structural abnormalities the atria, although molecular mechanisms are not well understood. Here, we used two‐dimensional polyacrylamide gel electrophoresis (2D PAGE) coupled nanoliquid chromatography‐tandem mass spectrometry (nanoLC‐MS/MS) to investigate proteins that dysregulated in atria from severe moderate when compared control. We found enzymes involved glycolysis,...