A5054737574- Genomics and Phylogenetic Studies
- Atmospheric and Environmental Gas Dynamics
- Atmospheric Ozone and Climate
- Microbial Community Ecology and Physiology
- Advanced Chemical Physics Studies
- Tardigrade Biology and Ecology
- Biocrusts and Microbial Ecology
- Spectroscopy and Laser Applications
- Ancient Egypt and Archaeology
- Multimedia Communication and Technology
- Surface and Thin Film Phenomena
- Protist diversity and phylogeny
- Telecommunications and Broadcasting Technologies
- Spaceflight effects on biology
- Electron and X-Ray Spectroscopy Techniques
- Image and Video Quality Assessment
- Biomedical Text Mining and Ontologies
- Genetic diversity and population structure
- RNA and protein synthesis mechanisms
- Gene expression and cancer classification
- Physics of Superconductivity and Magnetism
- Insect and Pesticide Research
- Molecular Junctions and Nanostructures
- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
Justus-Liebig-Universität Gießen
2019-2025
Giessen School of Theology
2023
University of Würzburg
2011-2020
Fraunhofer Institute for Molecular Biology and Applied Ecology
2017-2020
LOEWE Centre for Translational Biodiversity Genomics
2020
Foerster (Germany)
2019
Karlsruhe Institute of Technology
2010-2014
Leibniz-Institute for Food Systems Biology at the Technical University of Munich
2014
Garmisch-Partenkirchen Medical Center
2011-2013
Cancer Research UK
2009
Today, the base code of DNA is mostly determined through sequencing by synthesis as provided Illumina sequencers. Although highly accurate, resulting reads are short, making their analyses challenging. Recently, a new technology, single molecule real-time (SMRT) sequencing, was developed that could address these challenges, it generates several thousand bases. But, broad application has been hampered high error rate. Therefore, hybrid approaches use high-quality short to correct erroneous...
The internal transcribed spacer 2 (ITS2) is a well-established marker for phylogenetic analyses in eukaryotes. A reliable resource reference sequences and their secondary structures the ITS2 database (http://its2.bioapps.biozentrum.uni-wuerzburg.de/). However, was last updated 2011. Here, we present major update of underlying data almost doubling number entities. This increases taxa represented within all eukaryotic clades. Moreover, additional has been added to underrepresented groups some...
The internal transcribed spacer 2 (ITS2) is a widely used phylogenetic marker. In the past, it has mainly been for species level classifications. Nowadays, wider applicability becomes apparent. Here, conserved structure of RNA molecule plays vital role. We have developed ITS2 Database (http://its2.bioapps.biozentrum.uni-wuerzburg.de) which holds information about sequence, and taxonomic classification all in GenBank. new version, we use Hidden Markov models (HMMs) identification delineation...
In several studies, secondary structures of ribosomal genes have been used to improve the quality phylogenetic reconstructions. An extensive evaluation benefits structure, however, is lacking.This first study counter this deficiency. We inspected accuracy and robustness phylogenetics with individual by simulation experiments for artificial tree topologies up 18 taxa divergency levels in range typical studies. chose internal transcribed spacer 2 cistron as an exemplary marker region....
Many marine sponges are populated by dense and taxonomically diverse microbial consortia. We employed a metagenomics approach to unravel the differences in functional gene repertoire among three Mediterranean sponge species, Petrosia ficiformis, Sarcotragus foetidus, Aplysina aerophoba seawater. Different signatures were observed between seawater metagenomes with regard community composition, GC content, estimated bacterial genome size. Our analysis showed further pronounced for defense...
Most plants grow and develop by taking up nutrients from the soil while continuously under threat foraging animals. Carnivorous have turned tables capturing consuming nutrient-rich animal prey, enabling them to thrive in nutrient-poor soil. To better understand evolution of botanical carnivory, we compared draft genome Venus flytrap (Dionaea muscipula) with that its aquatic sister, waterwheel plant Aldrovanda vesiculosa, sundew Drosera spatulata. We identified an early whole-genome...
We demonstrate the application of orbital $k$-space tomography for analysis bonding occurring at metal-organic interfaces. Using angle-resolved photoelectron spectroscopy, we probe spatial structure highest occupied molecular and former lowest unoccupied (LUMO) one monolayer 3, 4, 9, 10-perylene-tetracarboxylic-dianhydride (PTCDA) on Ag(110) (111) surfaces and, in particular, influence hybridization between orbitals electronic states substrate. are able to quantify localize substrate...
Background Chloroplast-encoded genes (matK and rbcL) have been formally proposed for use in DNA barcoding efforts targeting embryophytes. Extending such a protocol to chlorophytan green algae, though, is fraught with problems including non homology (matK) heterogeneity that prevents the creation of universal PCR toolkit (rbcL). Some advocated nuclear-encoded, internal transcribed spacer two (ITS2) as an alternative traditional chloroplast markers. However, ITS2 broadly perceived be...
Whole genome alignments and comparative analysis are key methods in the quest of unraveling dynamics evolution. Interactive visualization exploration generated alignments, annotations, phylogenetic data important steps interpretation initial results. Limitations existing software inspired us to develop our new tool AliTV, which provides interactive whole alignments. AliTV reads multiple or automatically generates from provided data. Optional feature annotations phylo- genetic information...
Abstract Background Chloroplasts are intracellular organelles that enable plants to conduct photosynthesis. They arose through the symbiotic integration of a prokaryotic cell into an eukaryotic host and still contain their own genomes with distinct genomic information. Plastid accommodate essential genes regularly utilized in biotechnology or phylogenetics. Different assemblers able assess plastid genome have been developed. These often use data whole sequencing experiments, which usually...
“Bee pollen” is pollen collected from flowers by honey bees. It used the bees to nourish themselves, mainly providing royal jelly and brood food, but it also for human nutrition. For latter purpose, at hive entrance as pellets that bring hive. Bee has diverse bioactivities, thus been a health even medication in some countries. In this paper, we provide standard methods carrying out research on bee pollen. First, introduce method production storage of which assures quality product. Routine...
An ovel instrument for imaging ESCA is described. It based on a tandem arrangement of two hemispherical energy analysers used as an filter. The main spherical aberration (α 2 -term) the analyser corrected by antisymmetry configuration. kinetic range useable extends up to 1.6 keV; this compatible with Mg and Al Kα laboratory x-ray sources. First experiments chemical surface composition Cu0.98Bi0.02 polycrystal, GaAs/AlGaAs heterostructure Ag crystallites Si(111) have been performed using...
Abstract. Trends of column-averaged methane for the time period (1996, Sep 2011) are derived from mid-infrared (mid-IR) solar FTIR series at Zugspitze (47.42° N, 10.98° E, 2964 m a.s.l.) and Garmisch (47.48° 11.06° 743 a.s.l.). Trend analysis comprises a fit to de-seasonalized along with bootstrap resampling quantifying trend uncertainties. We find positive during [1996, 1998] 9.0 [3.2, 14.7] ppb yr−1 (95% confidence interval), an insignificant growth [1999, mid 2006] 0.8 [−0.1, 1.7]...
Abstract Background Tardigrades represent an animal phylum with extraordinary resistance to environmental stress. Results To gain insights into their stress-specific adaptation potential, major clusters of related and similar proteins are identified, as well specific functional delineated comparing all tardigrades individual species ( Milnesium tardigradum , Hypsibius dujardini Echiniscus testudo Tulinus stephaniae Richtersius coronifer ) elements in tardigrade mRNAs analysed. We find that...
Tardigrades are small, multicellular invertebrates which able to survive times of unfavourable environmental conditions using their well-known capability undergo cryptobiosis at any stage life cycle. Milnesium tardigradum has become a powerful model system for the analysis cryptobiosis. While some genetic information is already available proteome still be discovered.Here we present best our knowledge first comprehensive study on protein level. To establish reference map developed optimized...
Abstract. We present a strategy (MIR-GBM v1.0) for the retrieval of column-averaged dry-air mole fractions methane (XCH4) with precision <0.3% (1-σ diurnal variation, 7-min integration) and seasonal bias <0.14% from mid-infrared ground-based solar FTIR measurements Network Detection Atmospheric Composition Change (NDACC, comprising 22 stations). This makes NDACC data useful satellite validation inversion regional-scale sources sinks in addition to long-term trend analysis. Such...
Within Chlorophyceae the ITS2 secondary structure shows an unbranched helix I, except for 'Hydrodictyon' and 'Scenedesmus' clade having a ramified first helix. The latter two are classified within Sphaeropleales, characterised by directly opposed basal bodies in their flagellar apparatuses (DO-group). Previous studies could not resolve taxonomic position of 'Sphaeroplea' without ambiguity pivotal questions remain open: (1) Is DO-group monophyletic (2) is branched I apomorphic feature...
Semi-terrestrial tardigrades exhibit a remarkable tolerance to desiccation by entering state called anhydrobiosis. In this state, they show strong resistance against several kinds of physical extremes. Because the probable importance stress proteins during phases dehydration and rehydration, relative abundance transcripts coding for two alpha-crystallin heat-shock (Mt-sHsp17.2 Mt-sHsp19.5), as well Mt-sHsp10, Mt-Hsp60, Mt-Hsp70 Mt-Hsp90, were analysed in active anhydrobiotic species...
The phenomenon of desiccation tolerance, also called anhydrobiosis, involves the ability an organism to survive loss almost all cellular water without sustaining irreversible damage. Although there are several physiological, morphological and ecological studies on tardigrades, only limited DNA sequence information is available. Therefore, we explored transcriptome in active anhydrobiotic state tardigrade Milnesium tardigradum which has extraordinary tolerance freezing. In this study, present...
Abstract. Trends in the CO andC2H6 partial columns ~0–15 km) have been estimated from four European ground-based solar FTIR (Fourier Transform InfraRed) stations for 1996–2006 time period. The trends Jungfraujoch, Zugspitze, Harestua and Kiruna to −0.45 ± 0.16% yr−1, −1.00 0.24% −0.62 0.19 % yr−1 −0.61 respectively. corresponding C2H6 are −1.51 0.23% −2.11 0.30% −1.09 0.25% −1.14 0.18% yr−1. All presented with their 2-σ confidence intervals. To find possible reasons trends, global-scale EMEP...