A5090600655- Ion Transport and Channel Regulation
- Dialysis and Renal Disease Management
- Electrolyte and hormonal disorders
- Renal function and acid-base balance
- Neonatal Health and Biochemistry
- Biomedical Research and Pathophysiology
- Pediatric Urology and Nephrology Studies
- Central Venous Catheters and Hemodialysis
- Magnesium in Health and Disease
- Acute Kidney Injury Research
- Parathyroid Disorders and Treatments
- Ion channel regulation and function
- Neonatal Respiratory Health Research
- Birth, Development, and Health
- Hormonal Regulation and Hypertension
- Renal and related cancers
- Metabolism and Genetic Disorders
- Neonatal and fetal brain pathology
- Chronic Kidney Disease and Diabetes
- Sepsis Diagnosis and Treatment
- Muscle and Compartmental Disorders
- Eicosanoids and Hypertension Pharmacology
- Connexins and lens biology
- Vascular Procedures and Complications
- Drug Transport and Resistance Mechanisms
The University of Texas Southwestern Medical Center
2015-2025
Children's Medical Center
1997-2025
Texas Health Dallas
2024
University of Iowa Stead Family Children’s Hospital
2023
Children's Hospital Colorado
2023
University of Colorado Denver
2023
Children's Hospital Association
2023
Medical City Children's Hospital
2023
Johns Hopkins University
2023
Johns Hopkins Medicine
2023
Objectives: Acute kidney injury and fluid overload frequently necessitate initiation of continuous renal replacement therapy in critically ill patients admitted to the ICU. In this study, our primary objective was determine effect timing on ICU mortality children requiring support for management acute and/or overload. Design: Retrospective cohort study. Setting: Tertiary level, multidisciplinary PICU. Patients: Children who received from January 2000 through July 2009 were included Patients...
The proximal tubule contains an autonomous renin-angiotensin system that regulates transport independently of circulating angiotensin II. Androgens are known to increase expression angiotensinogen, but the effect androgens on is unknown. In this in vivo microperfusion study, we examined transport. volume reabsorptive rate Sprague-Dawley rats given dihydrotestosterone (DHT) injections was significantly higher than control vehicle (4.57 ± 0.31 vs. 3.31 0.23 nl·min −1 ·mm , P < 0.01)....
This in vitro microperfusion study examined the effects of growth hormone and insulin-like factor I (IGF-I) on proximal convoluted tubule (PCT) transport. Tubules were perfused with an ultrafiltrate-like solution bathed a serum-like albumin solution. Neither physiologic (5 x 10(-10) M), nor pharmacologic 10(-8) M) dose had effect PCT phosphate or bicarbonate transport, volume absorption. Addition 5 10(-9) M IGF-I, but not to bathing resulted increase (12-15%) no change absorption IGF-I...
The kidney has the highest abundance of cytochrome P-450 all extrahepatic organs. Within kidney, concentration is found in proximal tubule. Whether 20- or 19( S)-hydroxyeicosatetraenoic acid (HETE), major metabolites arachidonic tubule, affect transport this segment not been previously investigated. We examined direct effects and S)-HETE on volume absorption ( J v ) rabbit straight tubule (PST). Production 20-HETE by PST was demonstrated incubating microdissected tubules with [ 3...
Cellular cystine loading with dimethyl ester inhibits volume absorption, transepithelial potential difference, glucose transport, and bicarbonate transport in proximal convoluted tubules perfused vitro. This study examined the roles of ATP NaK ATPase this vitro model Fanconi syndrome cystinosis. Intracellular was measured using luciferin-luciferase assay. reduced by 60% incubated 0.5 mM for 15 min at 37 degrees C (P less than 0.001). Incubation loaded 1 exogenous increased intracellular to...
Cystic fibrosis (CF) is an exocrine disease affecting multiple organ systems. Patients with CF usually present respiratory or gastrointestinal abnormalities. This study presents a case of previously healthy 17-yr-old man who was diagnosed after presenting metabolic alkalosis and hypokalemia. The defect associated in the cystic transmembrane regulator (CFTR), which acts primarily as chloride channel. Partially functional CFTR may be less severe pulmonary manifestations, presented....
The cytochrome P450 (CYP) enzymes participate in a wide range of biochemical functions, including metabolism arachidonic acid and steroid hormones. Mouse CYP2J5 is abundant the kidney where its products, cis-epoxyeicosatrienoic acids (EETs), modulate sodium transport vascular tone. To define physiological role kidney, knockout mice were generated using conventional gene targeting approach. Cyp2j5 (-/-) develop normally exhibit no overt renal pathology. While EET biosynthesis was apparently...
Serum and glucocorticoid-regulated kinase 2 (sgk2) is 80% identical to the domain of sgk1, an important mediator mineralocorticoid-regulated sodium (Na + ) transport in distal nephron kidney. The expression pattern role renal function sgk2 are virtually uncharacterized. In situ hybridization immunohistochemistry rodent kidney coupled with real-time RT-PCR microdissected rat tubules showed robust proximal straight tubule thick ascending limb loop Henle. Sgk2 was minimal cells aquaporin-2...
ROMK channels are present in the cortical collecting ducts of kidney and responsible for K + secretion this nephron segment. Recent studies suggest that endocytosis is important regulation ducts. We investigated molecular mechanisms expressed Xenopus laevis oocytes cultured Madin-Darby canine cells. When plasma membrane insertion newly synthesized channel proteins was blocked by incubation with brefeldin A, currents decreased a half-time ∼6 h. Coexpression Lys44→Ala dominant-negative mutant...
Current techniques to morphologically characterize the processes of nephrogenesis and ureteric branching during kidney development have many limitations. Here, we used in vivo three-dimensional analysis study renal mice lacking fibroblast growth factor receptor 2 bud (Fgfr2(UB-/-)) littermate controls. We found that Fgfr2(UB-/-) more severe defects morphogenesis than previously reported, including significantly fewer branches tips control mice. Furthermore, these had decreased volume surface...
The present in vitro microperfusion study examined the effect of luminal angiotensin II on proximal convoluted tubule (PCT) volume absorption and bicarbonate transport. Neither 10 −11 M, −10 nor 2 × −8 M significantly affected PCT When tubules were first perfused with enalaprilat to inhibit endogenous production, addition increased (0.72 ± 0.08 vs. 0.86 0.07 nl ⋅ mm −1 min , P < 0.01) transport (52.3 3.7 67.9 4.2 pmol 0.01). Addition −6 losartan, an AT 1 inhibitor, perfusate inhibited...
We have recently demonstrated that the rates of both active and passive proximal straight tubule (PST) NaCl transport in neonatal rabbits were less than adults. In this segment entry across apical membrane is via parallel Na + /H Cl − /OH exchangers, which increases activity with maturation. The present vitro microperfusion study examined whether thyroid hormone plays a role maturational increase PST transport. Neonatal adult perfused high-chloride-low bicarbonate solution without organic...