A5026890116- Wound Healing and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Burn Injury Management and Outcomes
- Silk-based biomaterials and applications
- Bone Tissue Engineering Materials
- Proteoglycans and glycosaminoglycans research
- Collagen: Extraction and Characterization
- Pressure Ulcer Prevention and Management
- Hemostasis and retained surgical items
- Surgical Sutures and Adhesives
- Dermatologic Treatments and Research
- Tendon Structure and Treatment
- Reconstructive Surgery and Microvascular Techniques
- Liver physiology and pathology
- Thermoregulation and physiological responses
- Corneal Surgery and Treatments
- Cell Adhesion Molecules Research
- Diabetic Foot Ulcer Assessment and Management
- Melanoma and MAPK Pathways
- Hops Chemistry and Applications
- Hair Growth and Disorders
- Cutaneous Melanoma Detection and Management
United States Army Institute of Surgical Research
2015-2024
Defense Health Agency
2024
Oregon State University
2021
Amicrobe (United States)
2021
Smith & Nephew (United States)
2020
University of Georgia
2020
University of Miami
2018
Joint Base San Antonio
2018
Brooke Army Medical Center
2017
United States Army Medical Research and Development Command
2016
Thermal injuries are caused by exposure to a variety of sources, and split thickness skin grafts the gold standard treatment for severe burns; however, they may be impossible when there is no donor available. Large total body surface area burns leave patients with limited site availability create need treatments capable achieving early complete coverage that can also retain normal function. In this preclinical trial, two cellular tissue based products (CTPs) evaluated on twenty-four 5 × deep...
Large body surface area burns pose significant therapeutic challenges. Clinically, the extent and depth of burn injury may mandate use allograft for temporary wound coverage while autografts are serially harvested from same donor areas. The paucity sites in patients with involving large areas highlights need better skin substitutes that can achieve early complete retain normal durability minimal requirements. We have isolated autologous stem cells adipose layer surgically debrided burned...
Biologic substrates, prepared by decellularizing and solubilizing tissues, have been of great interest in the tissue engineering field because preservation complex biochemical constituents found native extracellular matrix (ECM). The integrity ECM is critical for cell behavior, adhesion, migration, differentiation, proliferation that turn affect homeostasis regeneration. Previous studies shown various processing methods a distinctive way affecting composition decellularized ECM. In this...
This work describes the differentiation of adipose-derived mesenchymal stem cells (ASC) in a composite hydrogel for use as vascularized dermal matrix. Our intent is that such construct could be utilized following large-surface-area burn wounds require extensive skin grafting and are limited by availability uninjured sites. To develop engineered replacement constructs, we pursuing ASC. We have established PEGylated fibrin gel can provide suitable environment proliferation ASC over 7 day time...
The objective of this study was to demonstrate that stem cells isolated from discarded skin obtained after debridement can be used with collagen and fibrin-based scaffolds develop a tissue-engineered vascularized dermal equivalent. Discarded tissue samples were collected severely burned patients undergoing wound debridement. Stem the adipose layer their growth immunophenotype characterized. To equivalent, debrided (dsASCs) added collagen-polyethylene glycol (PEG) bilayer hydrogel analyzed in...
Biomaterials capable of neutralizing specific cytokines could form the basis for treating a broad range conditions characterized by intense, local inflammation. Severe burns, spanning partial- to full-thickness dermis, can result in complications due acute inflammation that contributes burn progression, and early mediation may be key factor rescuing thermally injured tissue from secondary necrosis improve healing outcomes. In this work, we examined effects on progression influence...
Numerous techniques have been reported for preparing and sterilizing amniotic membrane (AM) use in clinical applications. However, these preparations either do not produce completely sterile tissue or are detrimental to molecules unique the matrix, thus compromising beneficial wound-healing properties of AM graft. The objective this work was a human graft using novel preparation technique involving supercritical carbon dioxide (SCCO2). subjected various sterilization treatment groups that...
Abstract Harvesting of autografts results in donor site morbidities and is limited scenarios such as large total body surface area burns. In these instances, coverage increased by meshing grafts at the expense delayed biologic closure. Moreover, graft increases likelihood contraction hypertrophic scarring, limits range motion, worsens cosmesis. Many tissue engineering technologies have touted promise adipose-derived stem cells (ASCs) for burn wounds. The primary objective current study was...
Infection control is necessary for improved burn wound regeneration. In this study contact wounds were induced on the dorsum of rats and infected with Pseudomonas aeruginosa (107cfu/ml saline) left overnight (12–14 hours) to establish infection. After 12 hours, treated PEGylated fibrin hydrogel containing 50 mgs silver sulfadiazine (SSD) loaded chitosan microsphere (SSD-CSM-FPEG). On day 9, SSD-CSM-FPEG further received adipose derived stem cell (5×104 ASCs cells/ml) embedded in hydrogel....
Integration of stem cells to injured tissues requires an appropriate delivery device and scaffolding system. In the present study we have developed in vitro strategy load release adipose-derived mesenchymal (ASC) from chitosan microspheres (CSM) into a collagen gel scaffold. Porous CSM uniform size composition were prepared used as cell carrier. ASC allowed attach infiltrate through microsphere pores. The number viable was counted vitro, using MTT Calcein acetoxymethyl ester (AM) assays, it...
Abstract Objectives A novel collagen-based dressing consisting of 2,3-dihydroxybenzoic-acid-modified gelatin microspheres loaded with doxycycline has previously been reported to address both infection and matrix degradation. In the present study potential benefits were investigated in an excisional wound model rats challenged Pseudomonas aeruginosa. Methods full-thick (1.5 times 1.5 cm) was created on dorsum induced by injecting 105 colony-forming units (CFU) P. The healing pattern assessed...
Abstract Major traumatic injuries to the body, such as large surface area burns, limit availability of autologous stem cell populations for wound repair. This report demonstrates that even after severe burn trauma resident cells present within subcutaneous adipose tissue survive and are available therapeutic uses. Debrided skin from wounded areas contains can yield approximately 1.5 × 105 2.5 per milliliter tissue. observation indicates tissue, which is normally discarded, could be a...
ABSTRACT For severe burn injuries, successful medical intervention is accomplished by rapidly and safely providing physical barriers that can cover damaged skin tissues, thereby preventing critical danger of extensive bleeding infection. Despite availability a large assortment wound coverage options, the etiology healing rather complex leading to significant defects in repair. The use cell‐mediated treatment approaches combination with bioengineered constructs may provide missing tool...