A5090181536- Vibrio bacteria research studies
- Yersinia bacterium, plague, ectoparasites research
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Influenza Virus Research Studies
- Salmonella and Campylobacter epidemiology
- Genomics and Phylogenetic Studies
- Zoonotic diseases and public health
- Oil and Gas Production Techniques
- COVID-19 epidemiological studies
- Metabolomics and Mass Spectrometry Studies
- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Identification and Quantification in Food
Rostov-on-Don Anti-plague Institute Rospotrebnadzor
2013-2025
Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2019
The aim of the review was to analyze and assess epidemiological situation on cholera in world Russia 2013–2022 make a forecast for 2023. Over period 2013–2022, 500 administrative territories affected by were identified various regions 71 countries Asia, Africa America (the Caribbean region) with formation 69 endemic foci 16, 41 12 countries, respectively. In 2022, 1 209 301 cases registered 36 world. intensity epidemic process Asian (Syria, Lebanon) increased. Unfavorable African continent...
Objective : retrospective analysis of the results monitoring studies environmental water bodies in Rostov-on-Don for presence V. cholerae period from 1989 to 2023 at stationary sampling points assigned Anti-Plague Institute Rospotrebnadzor, identification trends and features dynamics isolation biological properties isolated strains. Materials methods 2023, 4362 samples aquatic ecosystems were examined. The organization conduct research was carried out accordance with regulatory...
The aim of the work was to analyze peculiarities cholera epidemiological situation in world and Russia 2024; forecast for 2025. results proactive surveillance showed a continued upward trend morbidity despite decrease this indicator 2024 (762 830 cases 42 countries) as compared 2023 (816 235 47 countries). A special feature import polyantibiotic-resistant Vibrio cholerae O1 El Tor strain from Tanzania Island Mayotte. Active labor migration Russian Federation (RF) countries with unfavorable...
Carried out has been whole-genome sequencing of the Vibrio cholerae 81 El Tor Inaba strain DNA, isolated from Temernik River waters in territory Rostov-on-Don July, 2014. Identified is presence hybrid CTX prophage, which contains ctxB gene classical type ( ctxB1 allele), as well rstR type, tcpA with mutations coding and promoter regions tcpET CIRS null-mutation rtxA gene, extended deletion inside pandemicity island VSP-II. All stated above peculiarities are characteristic cholera vibrio...
Aim of the work – to assess epidemiological situation on cholera in 2020 and make a forecast for 2021 based monitoring data analysis morbidity around world period 2011–2020. During between 2011 2020, 4 413 988 cases were recorded 97 countries with general trend towards decrease incidence (coefficient accuracy approximation R 2 0.5705). However, due continuing epidemic manifestations endemic Asia, Africa America, these continents was characterized as unfavorable 2020. The emergence new...
Objective of the work was to compare results monitoring Vibrio cholerae strains isolated in territories federal districts, their constituent entities and from certain water bodies between 2006–2016. Based on methodology, using updated geoinformation system “Cholera 1989–2014”, a comparative study dynamics isolation biological properties 586 O1, O139 R-variants, collected surface administrative Russia, carried out. Eventually it is established that various serogroups are found territory all...
The aim of the work was to summarize results cholera monitoring in 2021, assess current trends development epidemic process, and predict epidemiological situation Russian Federation for 2022. It established that within period 2012–2021, 4117264 cases with spread infection across 83 countries on all continents were registered world there a downward trend incidence Asia Africa. dynamics monthly morbidity 2021 associated emergencies as factors risk. Epidemics outbreaks documented against...
Aim. Application of the authors’ GIS «Cholera 1989-2014» for systematization atoxigenic strains serogroup 01 cholera vibrios (ctxAB-tcpA-, ctxAB-tcpA+), isolated from aquatic objects environment by pheno- and genotype. Materials methods. A sample 304 Vibrio cholerae was studied. Isolation 39 genes related to pathogenicity carried out. Discrimination ability a set determined Simpson formula. Cluster analysis out UPGMA method. Results. Analysis multi-year data on V. in country’s subject using...
The current stage of development the seventh pandemic cholera in world is characterized by a tendency to widespread, registration outbreaks, epidemics and sporadic cases diseases associated with drifts, including single drifts this infection (without spreading) into Russia. territory Russia one most water-supplied countries world; however, some rivers, such as Volga, Ob, Amur, Don are among important economic arteries, therefore just these rivers were objects study for work. A comparative...
The aim of the work was to determine pheno- and genotypic features aquatic CTX + strain Vibrio cholerae isolated in 2023 a comparative bioinformatic analysis whole-genome sequencing data. Materials methods . Whole-genome performed on MiSeq (Illumina) MinIon Oxford Nanopore Technologies (ONT) platforms; hybrid assembly whole genome carried out using Trycycler algorithm; errors were eliminated by means Medaka algorithm Pilon program. Dendrogram construction bioinformatics with help scipy...
The aim of the study was to retrospectively analyze range variability antigenic properties and genotypic characteristics Vibrio cholerae R-variant strains atypical in terms agglutinability. Materials methods . 169 V. with agglutinability have been studied using “AmpliSens® cholerae-FL” test-system. determination O1 antigen carried out “Ig- О1/О139 – ELISA/dot-ELISA” reagent kit. Results discussion A retrospective analysis complex phenoand isolated from surface water bodies territories three...
Aim. To assess the prevalence of antimicrobial resistance Vibrio cholerae nonO1/nonO139 strains in water bodies Rostov-on-Don. Materials and methods. Sampling was carried out at fixed points open reservoirs Rostov-on-Don from May to September 2016 2017. Sensitivity/resistance V. antibacterial drugs (ABP) recommended for emergency prevention treatment cholera determined by serial dilution on Müller-Hinton agar. Results. 196 nonO1/ nonO139 were isolated 2016, 75 — When serotyping it found that...
The complication of cholera epidemic situation in Ukraine (2011) caused the reinforcement measures on monitoring Vibrio cholerae seawater Taganrog Bay framework for prevention spread infection over territory Rostov region and country. Phenotypic genotypic features 3 selected atoxigenic strains eltor O1 1 toxigenic strain V. Inaba № 301, containing hybrid prophage were characterized. With great probability there was established absence association this with outbreak within neighboring...
The goal is to study the features of biological properties Vibrio cholerae strains found in environmental objects (EO) on territory Rostov-on-Don from 1989 2018. Materials and methods . Bacteriological, molecular statistical were used 73 cholera vibrios O1, O139 1702 nonO1/nonO139 serogroups isolated over a 30-year period. Results discussion It was that Rostovon-Don, 78.0 % toxigenic all protection Russia, among which 2 genetically unchanged. V. O1 classical strain 72 El Tor found, 70...
The aim of the study was to analyze resistance antibacterial drugs Vibrio cholerae strains isolated from environmental water bodies on territory Russia in 2019. V.cholerae O1 El Tor (14) and nonO1/nonO139 were used this work. Sensitivity/resistance 11 determined using method serial dilutions a solid nutrient medium. presence drug genes real-time PCR. Fluctuations sensitivity/resistance found various years. phenotypic tetracycline trimethoprim/sulfamethoxazole correlated with tetR dfrA1 them....
Objective of the investigation was a comparative bioinformatics analysis Vibrio cholerae О1 Cef (CHO cell elongating factor) genes and proteins. Materials methods. 36 strains from Rostov-on-Don Research Anti-Plague Institute collection have been utilized. DNA sequencing conducted on MiSeq platform (Illumina); gene identification carried out by means BioEdit 7.2.5, BLASTN 2.2.29, BLASTP, MEGA 7, Vector NTI Advance 11 software programs. Results conclusions. The data obtained confirmed to be...
During more than 50 years, a heterogeneous population of Vibrio cholerae non-O1/non-O139 (NAGs) exists in Rostov region Russia, whose representatives periodically cause acute human intestinal infections; the last case was registered 2018. In 2022, sick child again revealed Rostov, from whom 2 NAG subcultures were isolated. Aim study: bioinformatics analysis whole genome sequences (WGSs) 2022 clinical isolates, genetic determinants pathogenicity factors and relevant products. Materials...
Identified are the peculiarities of biological properties and origin cholera vibrio O1 serogroup strains isolated from Rostov-on-Don water bodies in course surveillance 2008–2012. 41 atoxigenic V. cholerae have been 767 samples investigated. Stable tendency isolation increased numbers over time has demonstrated. In addition, under study detected genes additional pathogenicity factors, by PCR genotyping using specific primers for 45 nucleotide sequences associated with pathogenicity. The also...
The pheno- and genotypic properties of Vibrio cholerae El Tor cultures identified during monitoring for V. in the aquatic ecosystems Rostov-on-Don period 2003-2008 were studied extended range. In addition to known laboratory diagnostic techniques cholera, authors used a volume agglomeration test with antilipase diagnosticum, as well polymerase chain reaction using specific primers detect 30 nucleotide sequences genes associated pathogenicity. range characteristics study O1 was extended....
Twenty-four Vibrio cholerae O1 Eltor cultures and 700 V. nonO1/non/O139 strains were isolated during the 2003-2008 monitoring of surface water reservoirs waste waters Rostov-on-Don. The specific features circulation revealed in environmental objects area under study. These included a dynamic increasing trend for number annually cultures; detection single epidemiologically dangerous cholera epidemic well-being; no direct quantitative relationship between found serological groups nonO1/nonO139...
Determination of Vibrio cholerae affiliation to one or another serogroup may meet some difficulties in cases atypical agglutination with diagnostic cholera sera. The study genetic determinants that allows for identifying a is relevant task monitoring surface water body contamination vibrios. aim the work was compare structural organization (quantitative and qualitative gene composition) LPS clusters V. rough variants. Materials methods. We used Illumina MiSeq whole genome sequencing; SPAdes...
The aim of the work was to identify genotypic features clonal complexes formed by CTX–VPI + strains Vibrio cholerae isolated from water bodies Rostov Region in different years, based on bioinformatics analysis their whole genome sequences (WGSs). Materials and methods. Whole sequencing performed MiSeq (Illumina) platform; identification genetic determinants WGSs – means BioEdit, BLASTN, BLASTP, CARD, Vector NTI software packages. Results discussion. were almost identical each group 2002-,...