A5084892782- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Estrogen and related hormone effects
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- RNA modifications and cancer
- Reproductive Physiology in Livestock
- Genomics and Chromatin Dynamics
- Photosynthetic Processes and Mechanisms
- Signaling Pathways in Disease
- Cytokine Signaling Pathways and Interactions
- 14-3-3 protein interactions
- Cancer-related Molecular Pathways
- Medical Imaging and Pathology Studies
- Advanced Breast Cancer Therapies
- Heterotopic Ossification and Related Conditions
- Biocrusts and Microbial Ecology
- Cancer Genomics and Diagnostics
- HIV/AIDS drug development and treatment
- Endometrial and Cervical Cancer Treatments
- Cancer Cells and Metastasis
- Gynecological conditions and treatments
- Ubiquitin and proteasome pathways
- Algal biology and biofuel production
- Parathyroid Disorders and Treatments
Baylor College of Medicine
2013-2024
The University of Texas Southwestern Medical Center
2017-2019
Texas Children's Hospital
2015
University of New Orleans
2010
Successful embryo implantation requires a receptive endometrium. Poor uterine receptivity can account for failure in women who experience recurrent pregnancy loss or multiple rounds of unsuccessful vitro fertilization cycles. Here, we demonstrate that the transcription factor Forkhead Box O1 (FOXO1) is critical regulator endometrial vivo. Uterine ablation Foxo1 using progesterone receptor Cre (PgrCre) mouse model resulted infertility due to altered epithelial cell polarity and apoptosis,...
Decidualization is a complex process involving cellular proliferation and differentiation of the endometrial stroma that required to establish support pregnancy. Progesterone acting via its nuclear receptor, progesterone receptor (PGR), critical regulator decidualization known interact with certain members activator protein-1 (AP-1) family in regulation transcription. In this study, we identified cistrome transcriptome PGR AP-1 factors FOSL2 JUN be regulated by important process. Direct...
The forkhead box O1A (FOXO1) is an early-induced target of the protein kinase A pathway during decidualization human endometrial stromal cells (HESCs). In this study we identified cistrome and transcriptome FOXO1 its role as a transcriptional regulator progesterone receptor (PR). Direct targets were by integrating RNA sequencing with chromatin immunoprecipitation followed deep sequencing. Gene ontology analysis demonstrated that regulates subset genes in such those involved cancer, p53...
Men or mice with homozygous serine/threonine kinase 33 (
Progesterone, via the progesterone receptor (PGR), is essential for endometrial stromal cell decidualization, a cellular transformation event in which fibroblasts differentiate into decidual cells. Uterine decidualization supports embryo implantation and placentation as well subsequent events, together ensure successful pregnancy. Accordingly, impaired results not only failure or early fetal miscarriage, but also may lead to potential adverse outcomes all three pregnancy trimesters....
Phycobiliproteins are water-soluble, light-harvesting proteins that highly fluorescent due to linear tetrapyrrole chromophores, which makes them valuable as probes. Enzymes called bilin lyases usually attach these chromophores specific cysteine residues within the alpha and beta subunits via thioether linkages. A multiplasmid coexpression system was used recreate biosynthetic pathway for phycobiliproteins from cyanobacterium Synechococcus sp. strain PCC 7002 in Escherichia coli. This...
Abstract The biological processes that control endometrial receptivity and embryo implantation are critical for the successful outcome of pregnancy. endometrium is complex inner lining uterine wall under cyclical estrogen progesterone a site intimate contact between mother blastocyst. bone morphogenetic signaling (BMP) pathway highly conserved controls key cellular throughout pregnancy exerts intracellular effects via SMAD1/5 transcription factors. To delineate compartment–specific roles BMP...
The differentiation of endometrial stromal cells into decidual cells, termed decidualization, is an integral step in the establishment pregnancy. mitogen-activated protein kinase homolog, WNK lysine deficient 1 (WNK1), activated downstream epidermal growth factor receptor during decidualization. Primary human (HESCs) were subjected to small interfering RNA knockdown WNK1 followed by vitro This abrogated expression marker genes, insulin like binding (IGFBP1) and prolactin (PRL), prevented...
Epigenetic silencing of steroidogenic factor 1 (SF1) is lost in endometriosis, potentially contributing to de novo local steroidogenesis favoring inflammation and growth ectopic endometrial tissue. In this study, we examine the impact SF1 expression eutopic uterus by a novel mouse model that conditionally expresses endometrium. vivo promoted development enlarged glands attenuated estrogen progesterone responsiveness. Endometriosis induction autotransplantation uterine tissue mesenteric...
The ovarian hormones estrogen and progesterone orchestrate the transcriptional programs required to direct functions of uterus for initiation maintenance pregnancy. Estrogen, acting via receptor alpha, regulates gene expression by activating repressing distinct genes involved in signaling pathways that regulate cellular physiological responses including cell division, water influx, immune recruitment. Historically, these have been postulated reflect a biphasic response. In this study, we...
Estrogens, such as 17β- estradiol (E2), act through estrogen receptor alpha (ERα), a ligand-regulated transcription factor that binds across the genome to promote enhancer formation and regulate gene expression. ERα is expressed in approximately 70% of breast cancers, where it regulates genes involved mitogenic inflammatory pathways. We are exploring acetylation on lysines (K) 266 268, modifications we previously showed enhance DNA binding transcriptional activities biochemical cell-based...
Activin receptor type 1 (ACVR1; ALK2) and activin like (ACVRL1; ALK1) are transforming growth factor beta family receptors that integrate extracellular signals of bone morphogenic proteins (BMPs) activins into Mothers Against Decapentaplegic homolog 1/5 (SMAD1/SMAD5) signaling complexes. Several activating mutations in ALK2 implicated fibrodysplasia ossificans progressiva (FOP), diffuse intrinsic pontine gliomas, ependymomas. The R206H mutation is also present a subset endometrial tumors,...
Long noncoding RNAs (lncRNAs) have emerged as critical regulators of biological processes. However, the aberrant expression an isoform from same lncRNA gene could lead to RNA with altered functions due changes in their conformations, leading diseases. Here, we describe a detailed characterization that encodes long intergenic non-protein-coding 01016 (LINC01016, also known LncRNA1195) focus on its structure, exon usage, and human macaque tissues. In this study show it is among highly...
Abstract Long noncoding RNAs (lncRNAs) have emerged as critical regulators of biological processes. The constant expansion newly-identified lncRNA genes requires that each one be comprehensively annotated to understand its molecular functions. Here, we describe a detailed characterization the gene which encodes long intergenic non-protein coding RNA 01016 ( LINC01016 , a.k.a., LncRNA1195 ) with focus on structure, exon usage, and expression in human macaque tissues. In this study, show it is...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)