A5090382528- Immune cells in cancer
- Zebrafish Biomedical Research Applications
- Mitochondrial Function and Pathology
- Developmental Biology and Gene Regulation
- Neurogenesis and neuroplasticity mechanisms
- RNA modifications and cancer
- Peroxisome Proliferator-Activated Receptors
- Adipose Tissue and Metabolism
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Lysosomal Storage Disorders Research
- Autophagy in Disease and Therapy
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
University of Utah
2020-2023
Fred Hutch Cancer Center
2017-2018
University of Washington
2017-2018
Howard Hughes Medical Institute
2012
Recent studies reveal that lateral mitochondrial transfer, the movement of mitochondria from one cell to another, can affect cellular and tissue homeostasis. Most what we know about transfer stems bulk have led paradigm functional transferred restore bioenergetics revitalize functions recipient cells with damaged or non-functional networks. However, show also occurs between functioning endogenous networks, but mechanisms underlying how promote such sustained behavioral reprogramming remain...
An unresolved issue about many neurodegenerative diseases is why neurons are particularly sensitive to defects in ubiquitous cellular processes. One example Niemann Pick type C1, caused by cholesterol trafficking all cells, but where preferentially damaged. Understanding this selective failure limited the difficulty obtaining live human from affected patients. To solve problem, we generated with decreased function of NPC1 embryonic stem cells and used them test hypothesis that defective...
Peroxisomal biogenesis disorders (PBDs) are genetic of peroxisome and metabolism that characterized by profound developmental neurological phenotypes. The most severe class PBDs—Zellweger spectrum disorder (ZSD)—is caused mutations in peroxin genes result both non‐functional peroxisomes mitochondrial dysfunction. It is unclear, however, how defective contribute to impairment. In order understand the molecular basis this inter‐organellar relationship, we investigated fate peroxisomal mRNAs...
Abstract Lateral transfer of mitochondria occurs in many physiological and pathological conditions. Given that provide essential energy for cellular activities, mitochondrial is currently thought to promote the rescue damaged cells. We report between macrophages breast cancer cells, leading increased cell proliferation. Unexpectedly, transferred macrophage are dysfunctional, lacking membrane potential. Rather than performing accumulate reactive oxygen species which activates ERK signaling,...
Abstract Peroxisomal Biogenesis Disorders (PBDs) are a class of inherited metabolic disorders with profound neurological and other phenotypes. The most severe PBDs caused by mutations in peroxin genes, which result nonfunctional peroxisomes typically through impaired protein import. In order to better understand the molecular causes Zellweger Spectrum Disease (ZSD) -the -, we investigated fate peroxisomal mRNAs proteins ZSD model systems. We found that loss import has no effect on mRNA...
ABSTRACT The basic Helix-Loop-Helix transcription factor Atoh1 is required for the specification of multiple neuron types in mammalian hindbrain including tegmental, precerebellar output neurons and cerebellar granule neurons. How a single proneural gene specifies so many from progenitor zone, upper rhombic lip (URL), not known. Here we use zebrafish to explore role atoh1 neurogenesis. Using transgenic reporters show that atoh1c -expressing cells give rise tegmental cell populations that,...
During mammalian cerebellar development, a single Atoh1 basic‐helix‐loop‐helix transcription factor is required sequentially for the specification of multiple neuron types. The zebrafish genome encodes three paralagous atoh1 genes, atoh1a, atoh1b and atoh1c with overlapping but distinct spatio‐temporal expression patterns in developing cerebellum. (The presence extra paralogs fish genomes due to whole‐genome duplication at base teleost lineage). We have studied roles using transgenic...