Abstract A digestion mixture suitable for the decomposition of soil and plant materials is described. This based on sulphuric acid hydrogen peroxide as oxidants with addition lithium sulphate to elevate temperature selenium catalyst. The subsequent solution determination nitrogen, phosphorus most mineral ions. series tests have been carried out determine optimum conditions check elemental recoveries procedure compared alternative systems.

Organometallic ruthenium(II) arene anticancer complexes of the type [(η6-arene)Ru(II)(en)Cl][PF6] (en = ethylenediamine) specifically target guanine bases DNA oligomers and form monofunctional adducts (Morris, R., et al. J. Med. Chem. 2001). We have determined structures "piano-stool" [(η6-Bip)Ru(II)(en)Cl][PF6] (1, Bip biphenyl), [(η6-THA)Ru(II)(en)Cl][PF6] (2, THA 5,8,9,10-tetrahydroanthracene), [(η6-DHA)Ru(II)(en)Cl][PF6] (3, DHA 9,10-dihydroanthracene) with derivatives, in solid state by...

We have investigated the recognition of nucleic acid derivatives by organometallic ruthenium(II) arene anticancer complexes type [(η6-arene)Ru(II)(en)X] where en = ethylenediamine, biphenyl (Bip), tetrahydroanthracene (THA), dihydroanthracene (DHA), p-cymene (Cym) or benzene (Ben), X Cl- H2O using 1H, 31P and 15N (15N-en) NMR spectroscopy. For mononucleosides, {(η6-Bip)Ru(en)}2+ binds only to N7 guanosine, N1 inosine, N3 thymidine. Binding cytidine was weak, almost no binding adenosine...

Reactions of cisplatin (<i>cis</i> -[PtCl₂(NH₃)₂]) with albumin are thought to play an important role in the metabolism this anticancer drug. They investigated here via (i) labeling ¹⁵N and use two-dimensional¹H,¹⁵N NMR spectroscopy, (ii) comparison natural human serum recombinant (higher homogeneity SH content), (iii) chemical modification Cys, Met, His residues, (iv) reactions bound platinum thiourea, (v) gel filtration...

Abstract A series of robust iridium(I) complexes bearing a sterically encumbered N‐heterocyclic carbene ligand, alongside phosphine has been synthesised and investigated in hydrogen isotope exchange processes. These have allowed incorporation over range substrates with the use practically convenient deuterium tritium gas. Moreover, these active catalysts are capable to particularly high levels, whilst employing low catalyst loadings short reaction times. In addition this, new species shown...

Journal Article Paroxysmal Auricular Fibrillation: A Record of Two Hundred Patients Get access John Parkinson, Parkinson Search for other works by this author on: Oxford Academic PubMed Google Scholar Maurice Campbell QJM: An International Medicine, Volume os-24, Issue 93, October 1930, Pages 67–100, https://doi.org/10.1093/qjmed/os-24.93.67 Published: 01 1930 history Received: 26 August

Abstract The behavior of gelatin hydrogels is influenced by the charges located on amino acid side chains throughout molecules. presence and distribution ionisable influences surface activity ultimately determines material properties. Herein, we report influence pH mechanical properties as studied texture analysis supported data from polarimetry, zeta potential, titrations NMR experiments. When adjusted to more extreme values (pH 2 12), softer blocks were observed. However, at close...

Zinc is essential for many cellular processes, including DNA synthesis, transcription, and translation, but excess can be toxic. A zinc-induced gene, smtA , required normal zinc-tolerance in the cyanobacterium Synechococcus PCC 7942. Here we report that protein SmtA contains a cleft lined with Cys-sulfur His-imidazole ligands binds four zinc ions Zn 4 Cys 9 His 2 cluster. The thiolate sulfurs of five provide bridges between two ZnCys 3 sites, giving fused six-membered rings distorted boat...

Summary Zinc is essential but toxic in excess. Bacterial metallothionein, SmtA from Synechococcus PCC 7942, sequesters and detoxifies four zinc ions per molecule contains a finger structurally similar to eukaryotic GATA. The dearth of other reported bacterial metallothioneins has been surprising. Here we describe related (BmtA) Anabaena 7120, Pseudomonas aeruginosa putida that bind multiple with high stability towards protons. Thiol modification demonstrates cysteine coordinates all these...

Visible light irradiation of inert [PtIV(diam(m)ine)(diazide)] complexes leads to reductive elimination the azide ligands, production N2, and formation bifunctional adducts with guanine bases type which are lethal cancer cells (see scheme). Supporting information for this article is available on WWW under http://www.wiley-vch.de/contents/jc_2002/2003/z19849_s.pdf or from author. Please note: The publisher not responsible content functionality any supporting supplied by authors. Any queries...

The solution structure of the actinorhodin acyl carrier protein (act apo-ACP) from polyketide synthase (PKS) Streptomyces coelicolor A3(2) has been determined using 1H NMR spectroscopy, representing first component for which detailed structural information obtained. Twenty-four structures were generated by simulated annealing, employing 699 distance restraints and 94 dihedral angle restraints. is composed, principally, three major helices (1, 2, 4), a shorter helix (3) large loop region...

Seek and hide! An organometallic ruthenium arene anticancer complex with (pink ball) chelated by ethylenediamine (blue) is selective for guanine bases on DNA can bury the noncoordinated phenyl ring of its ligand (yellow) between in double helix. Supporting information this article available WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z602873_s.pdf or from author. Please note: The publisher not responsible content functionality any supporting supplied authors. Any queries (other...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMinor-groove recognition of the self-complementary duplex d(CGCGAATTCGCG)2 by Hoechst 33258: a high-field NMR studyJohn A. Parkinson, Jill Barber, Kenneth T. Douglas, John Rosamond, and Derek SharplesCite this: Biochemistry 1990, 29, 44, 10181–10190Publication Date (Print):November 1, 1990Publication History Published online1 May 2002Published inissue 1 November...

Turbocharged! A neutral dimeric molecule in crystal form, the diisopropylamido turbo-Grignard reagent "(iPr2N)MgCl⋅LiCl" (see structure; blue N, red O, green Mg, yellow Cl, black C) separates into several charged ate species dynamic exchange with each other THF solution as determined by a combination of EXSY and DOSY NMR studies. Detailed facts importance to specialist readers are published "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made...

In the present work we develop a novel approach for quantification of energetics C60 fullerene aggregation in aqueous media terms equilibrium constant KF. particular, it is shown that experimental determination magnitude KF possible only within framework 'up-scaled model', considering water solution as clusters. Using dynamic light scattering (DLS) data report value, = 56 000 M−1, which good agreement with existing theoretical estimates and results energetic analyses. It suggested proposed...

Melittin, the main peptide present in bee venom, has been proposed as having potential for anticancer therapy; addition of melittin to cisplatin, a first line treatment ovarian cancer, may increase therapeutic response cancer via synergy, resulting improved tolerability, reduced relapse, and decreased drug resistance. Thus, this study was designed compare metabolomic effects combination with cisplatin cisplatin-sensitive (A2780) resistant (A2780CR) cells. Liquid chromatography (LC) coupled...

Silk has emerged as an interesting candidate among protein-based nanocarriers due to its favorable properties, including biocompatibility and a broad spectrum of processing options tune particle critical quality attributes. The silk protein conformation during storage in the middle gland silkworm is modulated by various factors, most abundant metallic ion, calcium ion (Ca2+). Here, we report spiking liquid with ions modulate nanoparticle size. Conformational structural analyses demonstrated...

As platforms for the design of metal-based therapeutic and diagnostic agents, macrocycles are rigid enough to provide strong metal binding sites orient functional groups stereoselectively, yet flexible accommodate structural changes required induced-fit recognition biological targets. We consider Zn(II) complex bis-tetraazamacrocycle xylyl-bicyclam, a potent anti-HIV agent, by coreceptor CXCR4, G-protein-coupled receptor used HIV membrane fusion cell entry. NMR studies show that...

Organometallic chemistry offers novel concepts in structural diversity and molecular recognition that can be used drug design. Here, we consider DNA by η 6 -arene Ru(II) anticancer complexes an induced-fit mechanism. The stereochemistry of the dinuclear complex [((η -biphenyl)RuCl(en)) 2 -(CH ) ] + (3, en = ethylenediamine) was elucidated studies half unit [(η -biphenyl)RuCl(Et-en)] (2, where Et-en is Et(H)NCH CH NH ). structures separated \documentclass[12pt]{minimal} \usepackage{amsmath}...

The Ru(II) organometallic antitumor complex [(eta(6)-biphenyl)RuCl(en)][PF(6)] (1) reacts slowly with the amino acid L-cysteine (L-CysH(2)) in aqueous solution at 310 K. Reactions were followed over periods of up to 48 h using HPLC, electronic absorption spectroscopy, LC-ESI-MS, and 1D or 2D (1)H (15)N NMR spectroscopy. a 1 mM/2 mM (Ru/L-CysH(2)) ratio multiphasic acidic solutions (pH 5.1) appeared involve aquation as first step. Initially, 1:1 adducts involving substitution Cl by S-bound...

Subjecting ferrocene, ruthenocene, or osmocene to the synergic amide base sodium-magnesium tris(diisopropylamido) affords a unique homologous series of metallocene derivatives general formula [(M(C(5)H(3))(2))Na(4)Mg(4)(i-Pr(2)N)(8)] (where M = Fe (1), Ru (2), Os (3)). X-ray crystallographic studies 1-3 reveal common molecular "inverse crown" structure comprising 16-membered [(NaNMgN)(4)](4+) "host" ring and metallocenetetraide [M(C(5)H(3))(2)](4-) "guest" core, cleaved protons which are...