A5007353982- Ionosphere and magnetosphere dynamics
- Atmospheric Ozone and Climate
- Gene expression and cancer classification
- Geophysics and Gravity Measurements
- Gene Regulatory Network Analysis
- Molecular Biology Techniques and Applications
- Bioinformatics and Genomic Networks
- Solar and Space Plasma Dynamics
- Atmospheric chemistry and aerosols
- Atmospheric and Environmental Gas Dynamics
- Meteorological Phenomena and Simulations
- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- Genomic variations and chromosomal abnormalities
- Earthquake Detection and Analysis
- Cancer Mechanisms and Therapy
- Computational Drug Discovery Methods
- Seismic Waves and Analysis
- Microbial Metabolic Engineering and Bioproduction
- Cell Image Analysis Techniques
- Thyroid Cancer Diagnosis and Treatment
- NF-κB Signaling Pathways
- GNSS positioning and interference
- BRCA gene mutations in cancer
- Chromosomal and Genetic Variations
Eastern Virginia Medical School
2023-2025
Norfolk State University
2025
Siemens Healthcare (Germany)
2025
Old Dominion University
2025
University of Augsburg
2015-2024
Deutsches Zentrum für Luft- und Raumfahrt e. V. (DLR)
2015-2024
Computational Physics (United States)
1997-2023
Texas A&M University
2010-2022
Leibniz University Hannover
2015-2022
Translational Genomics Research Institute
2012-2021
Chimeric bacterial genes conferring resistance to aminoglycoside antibiotics have been inserted into the Agrobacterium tumefaciens tumor-inducing (Ti) plasmid and introduced plant cells by in vitro transformation techniques. The chimeric contain nopaline synthase 5' 3' regulatory regions joined for neomycin phosphotransferase type I or II. were cloned an intermediate vector, pMON120, pTiB6S3 recombination then petunia tobacco cocultivating A. with protoplast-derived cells. Southern...
Purpose To compare the progression-free survival (PFS) using a treatment regimen selected by molecular profiling (MP) of patient's tumor with PFS for most recent on which patient had experienced progression (ie, as his own control). Patients and Methods refractory metastatic cancer tissue samples submitted MP in two formats including formalin-fixed immunohistochemistry fluorescent situ hybridization assays immediately frozen oligonucleotide microarray (MA) gene expression (all performed...
We have shown that Wnt5A increases the motility of melanoma cells. To explore cellular pathways involving Wnt5A, we compared gain-of-function (WNT5A stable transfectants) versus loss-of-function (siRNA knockdown) WNT5A by microarray analysis. Increasing suppressed expression several genes, which were re-expressed after small interference RNA-mediated knockdown WNT5A. Genes affected include KISS-1, a metastasis suppressor, and CD44, involved in tumor cell homing during metastasis. This could...
Abstract Motivation: The receiver operator characteristic (ROC) curves are commonly used in biomedical applications to judge the performance of a discriminant across varying decision thresholds. estimated ROC curve depends on true positive rate (TPR) and false (FPR), with key metric being area under (AUC). With small samples these rates need be from training data, so natural question arises: How well do estimates AUC, TPR FPR compare metrics? Results: Through simulation study using data...
Alveolar rhabdomyosarcoma is an aggressive pediatric cancer of striated muscle characterized in 60% cases by a t(2;13)(q35;q14). This results the fusion PAX3, developmental transcription factor required for limb myogenesis, with FKHR, member forkhead family factors. The resultant PAX3-FKHR gene possesses transforming properties; however, effects this chimeric oncogene on expression are largely unknown. To investigate actions these factors, both Pax3 and were introduced into NIH 3T3 cells,...
Since early in the Atomic Age, biological indicators of radiation exposure have been sought, but currently available methods are not entirely satisfactory. Using cDNA microarray hybridization to discover new potential biomarkers, we identified genes expressed at increased levels human peripheral blood lymphocytes after ex vivo irradiation. We recently used this technique identify a large set ionizing radiation-responsive cell line (Oncogene 18, 3666-3672, 1999). The present markers was 24 h...
Background: Studies by comparative genomic hybridization (CGH) have shown that chromosomal region 17q23 is amplified in up to 20% of primary breast cancers. We used microarray analyses measure the expression levels genes this and explore their prognostic importance. Methods: A contained 4209 complementary DNA (cDNA) clones was identify are overexpressed MCF-7 cancer cell line as compared with normal mammary tissue. Fluorescence situ analyze copy number one gene, ribosomal protein S6 kinase...
The biological significance of DNA amplification in cancer is thought to be due the selection increased expression a single or few important genes. However, systematic surveys copy number and all genes within an amplified region genome have not been performed. Here we used combination molecular, genomic, microarray technologies identify target for 17q23, common breast cancers with poor prognosis. Construction 4-Mb genomic contig made it possible define two regions cell lines. Analysis 184...
Abstract The 60 cell lines of the National Cancer Institute Anticancer Drug Screen (NCI-60) constitute most extensively characterized in vitro cancer model. They have been tested for sensitivity to more than 100,000 potential chemotherapy agents and profiled at DNA, RNA, protein, functional, pharmacologic levels. We used NCI-60 three additional develop a database responses cells ionizing radiation. compared clonogenic survival, apoptosis, gene expression response by microarray. Although...
ABSTRACT Human herpesvirus 8 (HHV-8), a gammaherpesvirus implicated in Kaposi's sarcoma, primary effusion lymphoma, and Castleman's disease, encodes several pathogenically important cellular homologs. To define the HHV-8 transcription program, RNA obtained from latently infected body cavity-based lymphoma 1 cells induced to undergo lytic replication was used query custom DNA microarray containing nearly every known viral open reading frame. The patterns of gene expression offer insights into...
Multiple sclerosis (MS) and other T cell-mediated autoimmune diseases develop in individuals carrying a complex susceptibility trait, probably following exposure to various environmental triggers. Owing the presumed weak influence of single genes on disease predisposition recognized genetic heterogeneity disorders humans, candidate gene searches MS have been difficult. In an attempt identify molecular markers indicative status rather than for MS, we show that expression profiling peripheral...
Abstract Motivation: Intervention in a gene regulatory network is used to help it avoid undesirable states, such as those associated with disease. Several types of intervention have been studied the framework probabilistic Boolean (PBN), which essentially finite collection networks at any discrete time point state vector transitions according rules one constituent networks. For an instantaneously random PBN, governing randomly chosen each point. context-sensitive remains fixed for interval...