A5088403626- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Aquaculture disease management and microbiota
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Drug Transport and Resistance Mechanisms
- Iron Metabolism and Disorders
- Mycobacterium research and diagnosis
- Trace Elements in Health
- Plant Virus Research Studies
- Tuberculosis Research and Epidemiology
- Molecular Biology Techniques and Applications
- Pneumocystis jirovecii pneumonia detection and treatment
Université Toulouse III - Paul Sabatier
2021-2025
Centre National de la Recherche Scientifique
2019-2025
Institut de Pharmacologie et de Biologie Structurale
2021-2025
Université de Toulouse
2019-2021
École Nationale Vétérinaire de Toulouse
2019-2020
Interaction Hôtes Agents Pathogènes
2018-2020
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020
Université de Bordeaux
2019
Inserm
2019
Mycologie et Sécurité des Aliments
2019
Abstract The human pathogen Mycobacterium tuberculosis requires a P 1B -ATPase metal exporter, CtpC (Rv3270), for resistance to zinc poisoning. Here, we show that also depends on chaperone-like protein, PacL1 (Rv3269). contains transmembrane domain, cytoplasmic region with glutamine/alanine repeats and C-terminal metal-binding motif (MBM). binds Zn 2+ , but the MBM is required only at high concentrations. co-localizes in dynamic foci mycobacterial plasma membrane, two proteins form molecular...
Horizontal Gene Transfer was long thought to be marginal in Mycoplasma a large group of wall-less bacteria often portrayed as minimal cells because their reduced genomes (ca. 0.5 2.0 Mb) and limited metabolic pathways. This view recently challenged by the discovery conjugative exchanges chromosomal fragments that equally affected all parts chromosome via an unconventional mechanism, so whole mycoplasma genome is potentially mobile. By combining next generation sequencing classical mating...
The capacity of Mycoplasmas to engage in horizontal gene transfers has recently been highlighted. Despite their small genome, some these wall-less bacteria are able exchange multiple, large portions chromosome via a conjugative mechanism that does not conform canonical Hfr/oriT models. To understand the exact features underlying mycoplasma chromosomal transfer (MCT), extensive genomic analyses were performed at nucleotide level, using individual mating progenies derived from our model...
Metal ion homeostasis is crucial for bacterial pathogens to withstand metal-induced stress during infection. However, the mechanisms underlying resistance metal remain incompletely understood, particularly how bacteria coordinate responses simultaneous exposure multiple metals. Here, we uncover a previously unrecognized mechanism by which Mycobacterium tuberculosis , causative agent of tuberculosis, orchestrates coordinated response multi-metal stress. We demonstrate that M. assembles...
Post-transcriptional regulation plays important roles to fine-tune gene expression in bacteria. In particular, of type I toxin-antitoxin (TA) systems is achieved through sophisticated mechanisms involving toxin mRNA folding. Here, we set up a genetic approach decipher the molecular underpinnings behind TA Helicobacter pylori. We used lethality induced by chromosomal inactivation antitoxin select mutations that suppress toxicity. found single point are sufficient allow cell survival....
Abstract Post-transcriptional regulation plays important roles to finely tune gene expression in bacteria. In particular, of type I toxin-antitoxin (TA) systems is achieved through sophisticated mechanisms involving toxin mRNA folding. Here, we set up a genetic approach decipher the molecular underpinnings behind TA Helicobacter pylori . We used lethality induced by chromosomal inactivation antitoxin select mutations that suppress toxicity. found single point are sufficient allow cell...
ABSTRACT Transition metals are toxic at high concentrations. The P 1B -ATPase metal exporter CtpC/Rv3270 is required for resistance to zinc poisoning in the human pathogen Mycobacterium tuberculosis . Here, we discovered that also depends on chaperone-like protein PacL1/Rv3269. PacL1 bound Zn 2+ , but unlike and CtpC, metal-binding motif (MBM) was only co-localized with CtpC dynamic microdomains within mycobacterial plasma membrane. Microdomain formation did not require flotillins nor MBM....