A5049465666- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell Adhesion Molecules Research
- Inflammatory mediators and NSAID effects
- Bone Metabolism and Diseases
- S100 Proteins and Annexins
- Asthma and respiratory diseases
- Blood disorders and treatments
- Inflammasome and immune disorders
- Bone health and treatments
- Psoriasis: Treatment and Pathogenesis
- Sphingolipid Metabolism and Signaling
- Erythrocyte Function and Pathophysiology
- Gout, Hyperuricemia, Uric Acid
- Monoclonal and Polyclonal Antibodies Research
- Osteoarthritis Treatment and Mechanisms
- NF-κB Signaling Pathways
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- Protein Kinase Regulation and GTPase Signaling
- Eicosanoids and Hypertension Pharmacology
- Estrogen and related hormone effects
- Immune Cell Function and Interaction
- Bone health and osteoporosis research
- Systemic Lupus Erythematosus Research
Université Laval
2012-2024
Centre hospitalier universitaire de Québec
1991-2021
Centre hospitalier de l'Université Laval
1992-2019
Wilfrid Laurier University
1987-2014
Institute for Research in Immunology and Cancer
2007-2010
Centre de Recherche Dermatologique du Québec Métropolitain
2006
Institut National des Sciences Appliquées de Lyon
2003
Inserm
1996-2003
Direction des énergies
1998
CEA Paris-Saclay
1998
Osteoporosis is a common complication of long-term glucocorticoid therapy for which there no well-proved preventive or restorative treatment.
To evaluate the continued efficacy and safety of alendronate (ALN) for up to 2 years in patients receiving glucocorticoids.This is a 12-month extension previously completed 1-year trial daily ALN, performed effects ALN over total 66 men 142 women continuing receive at least 7.5 mg prednisone or equivalent daily. All received supplemental calcium vitamin D. The primary end point was mean percentage change lumbar spine bone mineral density (BMD) from baseline 24 months. Other outcomes included...
Significance On activation, blood platelets package components from their cytoplasm into microparticles (MPs), tiny vesicles released by cytoplasmic membrane budding and shedding. Given that MPs can impact other cellular lineages on internalization, we aimed to decipher the mechanisms promoting MP internalization recipients. We modeled neutrophils identified a predominant lipid, 12(S)-hydroxyeicosatetranoic acid, as mediator critical for promotion of internalization. were found inside...
Human peripheral blood mononuclear cells were isolated and assessed for the presence of contaminating polymorphonuclear leukocytes platelets.Incubations these cell isolates performed in or absence calcium ionophore A23 187 and/ l-14C-labeled unlabeled arachidonic acid.Using reverse phase high pressure liquid chromatography with simultaneous monitoring ultraviolet light absorption at 229 280 nm and, where appropriate, radioactivity, our studies reveal that human generate leukotrienes C4 B4...
To examine the role of chemokines, S100A8, and S100A9 in neutrophil accumulation induced by causative agent gout, monosodium urate monohydrate (MSU) crystals.MSU crystal-induced migration was studied murine air-pouch model. Release S100A9, S100A8/A9 response to MSU crystals quantified enzyme-linked immunosorbent assays. Recruited cells were counted following acetic blue staining, subpopulations characterized Wright-Giemsa staining cytospins.MSU neutrophils injection air pouch, which...
Abstract Functional links between bone remodeling and the immune system in chronic inflammatory arthritis are mediated, part, by ligand of receptor activator nuclear factor-kappa-B (RANK-L). Because neutrophils play a crucial role inflammation, goal this study was to determine whether proteins RANK/RANK-L pathway expressed synovial fluid (SF) from patients with rheumatoid (RA) characterize normal human blood neutrophils. The expression RANK-L, osteoprotegerin (OPG), RANK, tumor necrosis...
Proinflammatory agents were assessed for their capacity to stimulate the expression of inducible cyclooxygenase isoform (COX-2) in human neutrophils. A number agents, including PMA, opsonized bacteria and zymosan, LPS, GM-CSF, TNF-α, fMLP, induced COX-2 protein through signaling pathways involving transcription synthesis events. Northern blots showed that freshly isolated neutrophils expressed low levels mRNA, which rapidly increased after incubation with inflammatory agents....
Microfracture and drilling elicit a cartilage repair whose quality depends on subchondral bone repair. Alternatively activated (AA) macrophages express arginase-1, release angiogenic factors, could be potential mediators of trabecular repair.Chitosan-glycerol phosphate (GP)/blood implants arginase-1+ in vivo through neutrophil-dependent mechanisms improve drilled defects compared with alone.Controlled laboratory study.Bilateral trochlear were created 15 rabbits, microdrilled, treated or not...
Lysophosphatidic acid (LPA), via interaction with its G-protein coupled receptors, is involved in various pathological conditions. Extracellular LPA mainly produced by the enzyme autotaxin (ATX). Using fibroblast-like synoviocytes (FLS) isolated from synovial tissues of patients rheumatoid arthritis (RA), we studied expression profile LPA-induced cell migration, and interleukin (IL)-8 IL-6 production. We report that FLS express receptors LPA<sub>1-3</sub>. Moreover, exogenously applied...
Neutrophils contribute to demyelinating autoimmune diseases, yet their phenotype and functions have been elusive date. Here, we demonstrate that ICAM1 surface expression distinguishes extra- from intravascular neutrophils in the mouse CNS during experimental encephalomyelitis (EAE). Transcriptomic analysis of these 2 subpopulations indicated neutrophils, once extravasated, acquire macrophage-like properties, including potential for immunostimulation MHC class II-mediated antigen...
Nociceptin is a neuropeptide sharing sequence homology with classical opioid peptides but distinct pharmacological profile. Through activation of its receptor, NociR, nociceptin has been linked several physiological functions in the central nervous system including memory, locomotion, and processing pain signals. Recently, peripheral blood neutrophils (PMNs) were demonstrated to express functional result suggesting that additional remain be elucidated. The present study investigated...
We recently demonstrated that pathologically relevant inflam- matory microcrystals, namely triclinic monosodium urate (MSU) and calcium pyrophosphate dihydrate (CPPD) crystals, potently stimulate a characteristic protein tyrosine phos- phorylation pattern in human neutrophils differed from observed response to other soluble or particulate ago- nists.In this study, the effects of colchicine on phosphorylation induced by MSU CPPD crystals hu- man blood were investigated.Immunoblot analysis...
The generation of diradylglycerol (DRG) and phosphatidic acid (PdtOH) was investigated in neutrophils primed with granulocyte-macrophage colony-stimulating factor (GM-CSF). Mass accumulation DRG PdtOH measured using reversed-phase high performance liquid chromatography thin layer chromatography, respectively. GM-CSF had no direct effect on the levels DRG, but it increased late phase human stimulated FMLP. elevation mass peaked approximately 100 s clearly preceded that which at 150 s....
Leukotrienes (LT), mainly LTB4, have been shown recently to affect several functions of human lymphocytes in vitro, and they are regarded as putative modulators the immune response. Although it is recognized that neutrophils, eosinophils, monocyte-macrophages, mast cells can generate LTs, synthesis 5-lipoxygenase products by still subject a controversy. Human peripheral blood mononuclear leukocytes, nylon wool-purified lymphocytes, CD4+, CD4- T cells, large granular various fractions pure...
Psoriasis, a chronic autoimmune-related skin disease, involves both immune and non-immune cells like T keratinocytes. This study investigates the regulatory role of cells-keratinocyte interactions during psoriasis on factors production. Cytokines chemokines were evaluated by multiplex ELISA assays in an vitro model co-culture keratinocytes with lymphocytes. Keratinocytes from psoriatic lesions or healthy skin. lymphocytes volunteers. Psoriatic (PKs) alone generated concentrations tumor...
Abstract Introduction Monosodium urate (MSU) microcrystals present in bone tissues of chronic gout can be ingested by nonprofessional phagocytes like osteoblasts (OBs) that express NLRP3 (nucleotide-binding domain and leucine-rich repeat region containing family receptor protein 3). MSU is known to activate inflammasomes professional phagocytes. We have identified a new role for coupled autophagy MSU-stimulated human OBs. Methods Normal OBs cultured vitro were investigated their capacity...
Abstract Neutrophils produce IL-1 when stimulated by monosodium urate (MSU) or calcium pyrophosphate dihydrate (CPPD) crystals. also generate the IL-1R antagonist (IL-1Ra), especially incubated with granulocyte-macrophage CSF (GM-CSF) TNF-alpha. We studied simultaneous expression of and IL-1Ra GM-CSF- TNF-alpha-treated neutrophils activated MSU CPPD. GM-CSF TNF-alpha produced approximately 300 200 times more than IL-1, respectively. Suboptimal concentrations CPPD induced low amounts without...
Abstract Chronic crystal-associated arthropathies such as gout and pseudogout can lead to local bone destruction. Because osteoblasts, which orchestrate remodeling via soluble factors cell-to-cell interactions, have been described in contact with microcrystals, particularly uratic foci of gout, we hypothesized that microcrystals monosodium urate monohydrate (MSUM) calcium pyrophosphate dihydrate (CPPD) could alter osteoblastic functions. MSUM CPPD adhered human cells (hOB) vitro were partly...