Objectives This study aimed to investigate patients’ use of electronic Patient-Reported Outcome Measures (ePROMs) and understand the demographic clinical factors that may be correlated with patient responses BREAST-Q at preoperative stage breast cancer. The is a PROM in questionnaire format, developed validated assess satisfaction quality life for surgery patients.The hypothesis tested considering disparities geography, age education among responders essential capturing diverse population...

Background— Fibroblast growth factor 9 (FGF9) is secreted from bone marrow cells, which have been shown to improve systolic function after myocardial infarction (MI) in a clinical trial. FGF9 promotes cardiac vascularization during embryonic development but only weakly expressed the adult heart. Methods and Results— We used tetracycline-responsive binary transgene system based on α-myosin heavy chain promoter test whether conditional expression of myocardium supports adaptation MI. In...

The optimal time for flap anastomosis to an arteriovenous loop remains controversial. Whether perforator flaps and axially vascularized muscle or fasciocutaneous lead comparable outcomes in conjunction with loops has not been investigated.Medical records from 103 patients undergoing reconstruction (76 one-stage 27 two-stage) between 2007 2017 were reviewed. Postoperative compared one- two-stage reconstructions different types of free flaps.Rates thrombosis, major wound complications, failure...

Vascular shear stress promotes endothelial cell sprouting in vitro. The impact of hemodynamic forces on microRNA (miRNA) and gene expression within growing vascular networks vivo, however, remain poorly investigated. Arteriovenous (AV) shunts are an established model for induction neoangiogenesis vivo can serve as a tool analysis effects miRNA profiles over time. AV were microsurgically created rats explanted postoperative days 5, 10 15. Neoangiogenesis was confirmed by histologic...

Abstract Introduction The anterior lateral thigh (ALT) flap's vascular variability can lead to complications. Thus, the accuracy of numerous planning modalities has been studied. clinical relevance competing mapping methods, however, remains unknown. Therefore, we compared impact color‐coded Duplex ultrasonography and handheld audible Dopplers on surgical efficacy flap safety. Patients methods Forty‐four ALT flaps were included in this comparative retrospective monocentric study. Twelve...

Exposing a vein to altered hemodynamics by creating an arteriovenous (AV) shunt evokes considerable vessel formation that may be of therapeutic potential. However, it is unclear whether the introduction oscillatory flow and/or increase decisive. To distinguish between these mechanical stimuli we grafted femoral into arterial pathway contralateral limb in rats arterioarterial (AA) loop (n = 7). Alternatively, connected artery and using graft, whereby created AV-loop 27). Vessel loops were...

Autologous free flaps are the criterion standard for reconstructions of complex soft tissue defects; however, they limited by donor-site morbidities. The arteriovenous (AV) loop model enables generation constructs based on acellular dermal matrices with a functional microvasculature and minimal donor site morbidity. ideal scaffold AV loop-based engineering has not been determined.AV loops were placed into subcutaneous isolation chambers filled either collagen-elastin or...

Gap junctions are involved in vascular growth and their expression pattern is modulated response to hemodynamic conditions. They clusters of intercellular channels formed by connexins (Cx) which four subtypes expressed the cardiovascular system, namely Cx37, Cx40, Cx43 Cx45. We hypothesize that high flow conditions affect Cx vivo. To test this hypothesis, hemodynamics subsequent changes were studied an angioinductive rat arteriovenous (AV) loop model. Fifteen days after interposition a...

Connexins (Cx) form gap junctions (GJ) and allow for intercellular communication. However, these proteins also modulate gene expression, growth, cell migration. The downregulation of Cx43 impairs endothelial migration angiogenetic potential. Conversely, expression is upregulated in an vivo angiogenesis model relying on hemodynamic forces. We studied the effects tube formation proliferation HUVECs examined its dependency GJ Expectedly, communication assessed by dye transfer was linked to...

Background: Soft tissue free flap reconstruction of upper extremities has proven to be reliable and essential for limb salvage function. Nevertheless, comparative data regarding outcome are still lacking. The present study aimed compare procedural features individual complication rates different flaps used extremity reconstruction. Methods: authors evaluated retrospectively the results 164 in 149 patients with defects. Chart reviews were performed from April 2000 June 2014, analyzing...

Immunglobulin E ( I g ) production is tightly regulated at the cellular and genetic levels believed to be central allergy development. At least two pathways exist that lead systemic anaphylaxis reactions in vivo: ‐sensitized mast cells G 1‐sensitized basophils. Passive anaphylaxis, by application of allergen allergen‐specific antibodies mice, indicates a differential contribution immunoglobulin isotypes anaphylaxis. However, analysis dynamic immunization‐mediated antibody response difficult....

Summary Introduction The standardized characterization of angiogenesis is crucial in the field tissue engineering as sufficient blood supply limiting factor mass transfer. However, reliable algorithms that provide a straight forward and observer‐independent assessment new vessel formation are still lacking. We propose an automatic quantitative method (including downloadable source code) to analyze vascularization using two‐dimensional microscopic images histological cross‐sections advanced...

In order to introduce bone tissue engineering the field of oncological reconstruction, we are investigating for first time effect various doses ionizing irradiation on axially vascularized constructs. Synthetic constructs were created and implanted in 32 Lewis rats. Each construct was through an arteriovenous loop made by direct anastomosis saphenous vessels. After 2 weeks, animals received 9 Gy, 12 Gy 15 accordingly classified groups I, II III, respectively. Group IV not irradiated acted as...

The arteriovenous (AV) loop model permits the creation of significant volumes axially vascularized tissue that represents an alternative to conventional free flaps, circumventing their common limitations. However, such AV loop-based flaps have never before been examined in standardized animal models with respect suitability for reconstruction critical bone-exposing defects. In course our preliminary studies, we implemented a novel defect rats provides and wound conditions evaluated whether...

Fractionated fat has been shown to promote dermal regeneration; however, the use of grafting for reconstruction soft-tissue defects is limited because volume loss over time. The authors have developed a novel approach engineering vascularized soft tissue using an injectable nanofiber hydrogel composite enriched with fractionated fat.Fractionated was generated by emulsification groin pads from rats and mixed in 3:1 ratio (nanofiber fat). Nanofiber or alone placed into isolation chambers...

The aim of this in vivo study was to gather quantitative information on the three-dimensional morphology a new vascular network under influence angioactive growth factors. For purpose, arteriovenous loop model used 10 Lewis rats generate bioartificial assembly by means induction. In model, an isolated organoid is created medial thigh animal methods tissue engineering. A fibrin gel containing endothelial factor (VEGF(165)) and basic fibroblastic (bFGF) as matrix effect group (GF+). Fibrin...

Background: The placement of arteriovenous loops can enable microvascular anastomoses free flaps when recipient vessels are scarce. In animal models, elevated fluid shear stress in promotes neoangiogenesis. Anecdotal reports patients indicate that vein grafts used flap reconstructions ischemic lower extremities able to induce capillary formation. However, flow-stimulated angiogenesis has never been systematically investigated humans, and it is unclear whether alters proangiogenic signaling...

Background Limb salvage in patients with peripheral vascular disease (PVD) and soft tissue defects often requires both a restoration of blood flow to the lower extremity coverage. Outcomes free transfer may be affected by vein grafts, which can used for placement an autologous venous bypass or arteriovenous (AV) loop leading different hemodynamic situations. The purpose this study was investigate whether flap anastomosis AV performed comparable results. Methods We matched‐pair analysis 22...

Abstract Introduction We reported on the superiority of preoperative Duplex mapping (“Duplex”) over audible Dopplers (“Doppler”) in anterolateral thigh perforator (ALT) free flaps for upper extremity reconstruction. To corroborate our findings a larger cohort, we conducted this present study focusing surgical efficiency and patient safety. Methods 150 consecutive ALT were divided into 65 cases versus 85 Doppler controls. first compared demographics, operative details, defect flap...

In vivo tissue engineering (TE) techniques like the AV loop model provide an isolated and well-defined microenvironment to study angiogenesis-related cell interactions. Functional visualization of microvascular network within these artificial constructs is crucial for fundamental understanding vessel formation identify underlying key regulatory mechanisms. To facilitate tracking advanced fluorescence imaging are required. We studied suitability microporous polylactic acid (PLA) scaffolds...

Objective: To develop a novel approach for tissue engineering of soft-tissue flaps suitable free microsurgical transfer, using an injectable nanofiber hydrogel composite (NHC) vascularized by arteriovenous (AV) loop. Approach: A rat AV loop model was used flaps. NHC or collagen-elastin (CE) scaffolds were implanted into isolation chambers together with and explanted after 15 days. Saphenous veins the as controls. Neoangiogenesis, ultrastructure, protein expression SYNJ2BP, EPHA2, FOXC1...