Non-small-cell lung cancer (NSCLC) is the primary form of globally and remains a leading cause mortality. Anaplastic lymphoma kinase (ALK) mutations, such as I1171N + L1198H, have been discovered to confer resistance current ALK inhibitors, reducing their therapeutic effectiveness. Addressing drug necessitates exploring selective inhibitors for innovative approaches. In this study, structure-based pharmacophore model, using ALK-approved was developed screen an In-house database potential...

Introduction: The Macrophage Migration Inhibitory Factor (MIF), a key pro-inflammatory mediator, is responsible for modulating immune responses. An array of inflammatory and autoimmune diseases has been linked to the dysregulated activity MIF. significance in physiological as well pathophysiological phenomena underscores potential MIF an attractive target with pharmacological relevance. Extensive research past uncovered number inhibitors, while ISO-1, or (S,...

Dental caries is a multifactorial, biofilm-mediated disease primarily caused by Streptococcus mutans, key etiological agent. This bacterium secretes extracellular enzymes known as glucosyltransferases (Gtfs), also termed glucansucrases, which play pivotal role in the synthesis of exopolysaccharides through metabolism dietary sucrose. These provide binding sites for attachment and colonization other microorganisms, contributing to initiation progression dental caries. study investigates...

Transmembrane protease serine 2 (TMPRSS2) has been identified as a critical key for the entry of coronaviruses into human cells by cleaving and activating spike protein SARS-CoV-2. To block TMPRSS2 function, 18 approved drugs, containing guanidine group were tested against TMPRSS2's ectodomain (7MEQ). Among these Famotidine, Argatroban, Guanadrel Guanethidine strongly binds with S1 pocket estimated Fullfitness energies −1847.12, −1630.87, −1605.81 −1600.52 kcal/mol, respectively. A...

Abstract Free Fatty Acid Receptor 4 (FFAR4), a G-protein-coupled receptor, is responsible for triggering intracellular signaling pathways that regulate various physiological processes. FFAR4 agonists are associated with enhancing insulin release and mitigating the atherogenic, obesogenic, pro-carcinogenic, pro-diabetogenic effects, normally free fatty acids bound to FFAR4. In this research, molecular structure-based machine-learning techniques were employed evaluate compounds as potential...

JAK2 mutation plays a clinically significant role in the pathogenesis of acute lymphoblastic leukemia (ALL) by enhancing its oncogenicity. The study aimed to characterize molecular pathology and computational profile an ALL cohort Pakistani origin.

Cytogenetics is irrefutably one of the most significant prognostic markers which have imparted ease and feasibility in classification acute leukemia. It an essential tool major treatment protocols for all hematological malignancies. This study aimed to see frequency different cytogenetic aberrations children with B-ALL, their association risk factors, impact on post-induction remission status. A retrospective was conducted Hematology department The Indus Hospital, Karachi. All newly...

The non-structural protein 1 (NS1), a viral toxin, is linked to serious consequences of dengue. Given its crucial role in the life cycle, NS1 has been primary target for antiviral drug development. This study elucidates mechanistic impact specific mutations, V220D and A248V, identified by Tan colleagues as potential inhibitors replication. We developed an integrated computational framework analyze these mutations relative native protein. Using conventional all-atom molecular dynamic...

A novel strain of coronavirus, namely, SARS-CoV-2 identified in Wuhan city China December 2019, continues to spread at a rapid rate worldwide. There are no specific therapies available and investigations regarding the treatment this disease still lacking. In order identify potent inhibitor, we performed blind docking studies on main virus protease M pro with eight approved drugs belonging four pharmacological classes such as: anti-malarial, anti-bacterial, anti-infective anti-histamine....

<p></p><p>A novel strain of coronavirus, namely, SARS-CoV-2 identified in Wuhan city China December 2019, continues to spread at a rapid rate worldwide. There are no specific therapies available and investigations regarding the treatment this disease still lacking. In order identify potent inhibitor, we performed blind docking studies on main virus protease M<sup>pro</sup> with eight approved drugs belonging four pharmacological classes such as: anti-malarial,...

Background: Under the present situations, where health improvement has become major concern, food supplements, augmenting is a matter of interest. Presently, concept "functional foods" possessing medicinal attributes been reborn as probiotics. Acid-tolerant and Gram-positive Lactic acid bacteria (LAB), being class probiotics, possess health-promoting characteristics, provide better with robust immune system, treat various digestive neurological disorders. Hence, aim study was isolation...

Diabetes results in substantial disabilities, diminished quality of life, and mortality that imposes a huge economic burden on societies governments worldwide. Despite the absence specific oral therapies at present, there exists an urgent requirement to develop novel drug for treatment diabetes mellitus. The membrane protein sodium glucose co-transporters (SGLT1) present captivating therapeutic target diabetes, given its pivotal role facilitating absorption small intestine, offering immense...

Aims: Polycythemia refers to an increased production of red blood cells from bone marrow. It is one the frequent reasons for a hematology consultation. The present work aimed examine causes, clinical manifestations, prevalence, and diverse patterns in laboratory parameters among patients with polycythemia attending our outpatient clinics at Indus Hospital Health Network.
 Study Design: retrospective observational study which included all cases polycythemia.
 Place Duration Study:...