A5028722117- Plant-Microbe Interactions and Immunity
- Genetic Mapping and Diversity in Plants and Animals
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Genetics, Bioinformatics, and Biomedical Research
- Ophthalmology and Visual Impairment Studies
- Plant Molecular Biology Research
- Innovative Teaching Methods
- Protist diversity and phylogeny
- Various Chemistry Research Topics
- Genomics and Phylogenetic Studies
- Plant Parasitism and Resistance
- Retinopathy of Prematurity Studies
- Experimental Learning in Engineering
- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Crop Yield and Soil Fertility
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- GABA and Rice Research
- Chromosomal and Genetic Variations
- Global Maternal and Child Health
- Science Education and Pedagogy
- Island Studies and Pacific Affairs
- Academic Publishing and Open Access
University of Nebraska–Lincoln
2005-2023
Creighton University
2009-2017
Plant (United States)
2005
Cornell University
1998-2002
University of Nevada, Las Vegas
1999-2002
United States Department of Agriculture
2000-2002
Western Regional Research Center
2000
National Institute for Public Health and the Environment
1999
New York State College of Agriculture & Life Sciences
1998
The plant pathogenic bacterium Pseudomonas syringae is divided into pathovars differing in host specificity, with P. pv. ( Psy ) and tomato Pto representing particularly divergent pathovars. hrp / hrc genes encode a type III protein secretion system that appears to translocate Avr Hop effector proteins cells. DNA sequence analysis of the regions 61, B728a, DC3000 has revealed Hrp pathogenicity island (Pai) tripartite mosaic structure. gene cluster conserved all three strains flanked by...
The ability of Pseudomonas syringae pv. tomato DC3000 to parasitize and Arabidopsis thaliana depends on genes activated by the HrpL alternative sigma factor. To support various functional genomic analyses DC3000, specifically, identify involved in pathogenesis, we developed a draft sequence used an iterative process involving computational gene expression techniques virulence-implicated downstream HrpL-responsive promoters. Hypersensitive response pathogenicity (Hrp) promoters are known...
ABSTRACT We present here data showing that the Avr proteins HrmA and AvrPto are secreted in culture via native Hrp pathways from Pseudomonas syringae pathovars produce these proteins. Moreover, their secretion is strongly affected by temperature pH of medium. Both were at highest amounts when was between 18 22°C medium 6.0. In contrast, did not affect HrpZ. HrpZ secretion, but as it HrmA. This finding suggests there least two classes travel P. pathway: putative system accessory proteins,...
Abstract Background Although there have been numerous studies describing plant growth systems for root exudate collection, a common limitation is that these require disruption of the system to facilitate collection. Here, we present newly designed semi-hydroponic uses glass beads as solid support simulate soil impedance, which combined with drip irrigation, facilitates healthy maize plants, collection and analysis exudates, phenotyping roots minimal disturbance or damage. Results This was...
Abstract Background Transcription bridges genetic information and phenotypes. Here, we evaluated how changes in transcriptional regulation enable maize ( Zea mays ), a crop originally domesticated the tropics, to adapt temperate environments. Result We generated 572 unique RNA-seq datasets from roots of 340 genotypes. Genes involved core processes such as cell division, chromosome organization cytoskeleton showed lower heritability gene expression, while genes anti-oxidation activity...
Abstract Small RNA-guided gene silencing is an evolutionarily conserved process that operates by a variety of molecular mechanisms. In multicellular eukaryotes, the core components RNA-mediated have significantly expanded and diversified, resulting in partly distinct pathways for epigenetic control expression genomic parasites. contrast, many unicellular organisms with small nuclear genomes seem to lost entirely RNA-silencing machinery or retained only basic set components. We report here...
Understanding the relationship between molecular structure and function represents an important goal of undergraduate life sciences. Although evidence suggests that handling physical models supports gains in student understanding structure-function relationships, such have not been widely implemented biochemistry classrooms. Three-dimensional (3D) printing emerging cost-effective means producing to help students investigate concepts. We developed three interactive learning modules with...
Abstract Root exudates are important for shaping root-associated microbiomes. However, studies on a wider range of metabolites in required comprehensive understanding about their influence microbial communities. We identified maize inbred lines that differ exudate concentrations 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) and γ-aminobutyric acid (GABA) using semi-hydroponic system. These were grown the field to determine changes diversity gene expression due varying DIMBOA GABA 16S...
Interactions between plant-associated microorganisms play important roles in suppressing plant diseases and enhancing growth development. While competition bacteria pathogens has long been thought to be an means of microbiologically, unequivocal evidence supporting such a mechanism lacking. We present here that for plant-derived unsaturated long-chain fatty acids the biological control bacterium Enterobacter cloacae seed-rotting oomycete, Pythium ultimum, results disease suppression. Since...
Abstract Histone Lys methylation plays an important role in determining chromatin states and is mostly catalyzed by SET domain–containing proteins. The outcome, transcriptional repression or activation, depends on the methylated histone residue, degree of methylation, context. Dimethylation trimethylation H3 4 (H3K4me2 H3K4me3) has been correlated with transcriptionally competent/active genes. However, H3K4 also implicated gene silencing. This dualistic nature methyl mark thus far remained...
ABSTRACT Pseudomonas syringae is a gram-negative bacterial plant pathogen that dependent on type III protein secretion system (TTSS) and the effector proteins it translocates into cells for pathogenicity. The P. TTSS encoded by hrp-hrc genes reside in central region of pathogenicity island (Pai). Flanking one side this Pai exchangeable locus (EEL). We characterized transcriptional expression open reading frames (ORFs) within EEL pv. tomato DC3000. One these ORFs, PSPTO1406 ( hopB1 )...
<title>Abstract</title> <bold>Background</bold> Blindness and low vision in children remain significant public health concerns, particularly low- middle-income countries (LMICs) like Malawi, a key region Southern Africa. These conditions are often underdiagnosed undertreated due to limited access specialized services, spectacles assistive devices, posing critical challenges equity. The Childhood Low Vision Program (CBLVP) Northern Malawi aimed address gaps through capacity building,...
Strains of Enterobacter cloacae and other seed-associated bacteria, effective in suppressing Pythium seed rot damping-off, were tested for their ability to reduce the stimulatory activity (STA) plant exudates sporangia seed-rotting fungus, ultimum. Our results have shown that E. can utilize exudate from a number species as sole carbon energy source and, at same time, rapidly STA P. ultimum sporangia. At higher cell densities cloacae, was inactivated more than lower densities. Concentrates...
Summary Pseudomonas syringae uses a type III protein secretion system encoded by the Hrp pathogenicity island (Pai) to translocate effector proteins into plant cells. One of these is HopPsyA. A small open reading frame (ORF), named shcA , precedes hopPsyA gene in Pai P. s. 61. The predicted amino acid sequence shares general characteristics with chaperones used systems animal pathogens. functionally non‐polar deletion 61 resulted loss detectable HopPsyA supernatant fractions, consistent ShcA...
SET domain-containing proteins of the SU(VAR)3-9 class are major regulators heterochromatin in several eukaryotes, including mammals, insects, plants and fungi. The function these polypeptides is mediated, at least part, by their ability to methylate histone H3 on lysine 9 (H3K9). Indeed, mutants defective have implicated di- and/or trimethyl H3K9 formation maintenance across eukaryotic spectrum. Yet, biological significance monomethyl has remained unclear because lack exclusively this...
Understanding metabolic function requires knowledge of the dynamics, interdependence, and regulation networks. However, multiple professional societies have recognized that most undergraduate biochemistry students acquire only a surface-level understanding metabolism. We hypothesized guiding through interactive computer simulations systems would increase their ability to recognize how individual interactions between components affect behavior system under different conditions. The were...
3D printing represents an emerging technology with significant potential to advance life-science education by allowing students directly explore the relationship between macromolecular structure and function. In this article supplemental video guide, we describe our development of a model-based instructional module on DNA supercoiling outline practical tips for implementing models in undergraduate classrooms. We also present procedure design print dynamic classroom use. Furthermore,...
Abstract Ensuring undergraduate students become proficient in relating protein structure to biological function has important implications. With current two‐dimensional (2D) methods of teaching, frequently develop misconceptions, including that proteins contain a lot empty space, bond angles for different amino acids can rotate equally, and product inhibition is equivalent allostery. To help translate 2D images 3D molecules assign biochemical meaning physical structures, we designed three...
ABSTRACT Transcription bridges genetic information and phenotypes. Here, we evaluated how changes in transcriptional regulation enable maize ( Zea mays ), a crop originally domesticated the tropics, to adapt temperate environments. We generated 572 unique RNA-seq datasets from roots of 340 genotypes. Genes involved core processes such as cell division, chromosome organization cytoskeleton showed lower heritability gene expression. While genes anti-oxidation activity exhibited higher...