A5046516131- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Developmental Biology and Gene Regulation
- Bone Metabolism and Diseases
- Bone health and treatments
- TGF-β signaling in diseases
- Plant Molecular Biology Research
- RNA Research and Splicing
- Genetics, Bioinformatics, and Biomedical Research
- Cancer-related gene regulation
- Astronomical Observations and Instrumentation
- Geophysics and Gravity Measurements
- MicroRNA in disease regulation
- Heterotopic Ossification and Related Conditions
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Gamma-ray bursts and supernovae
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Curcumin's Biomedical Applications
- Wnt/β-catenin signaling in development and cancer
- Trace Elements in Health
- Animal Genetics and Reproduction
- Enzyme Structure and Function
- Cell Image Analysis Techniques
University of Geneva
2016-2025
Universitat de Barcelona
2010-2016
Institut d'Investigació Biomédica de Bellvitge
2010-2016
Bellvitge University Hospital
2011
The mammalian HoxD cluster lies between two topologically associating domains (TADs) matching distinct enhancer-rich regulatory landscapes. During limb development, the telomeric TAD controls early transcription of Hoxd genes in forearm cells, whereas centromeric subsequently regulates more posterior digit cells. Therefore, boundary prevents terminal Hoxd13 gene from responding to enhancers, thereby allowing proper patterning. To assess nature and function this CTCF-rich DNA region embryos,...
During vertebrate limb development, Hoxd genes are regulated following a bimodal strategy involving two topologically associating domains (TADs) located on either side of the gene cluster. These regulatory landscapes alternatively control different subsets targets, first into arm and subsequently digits. We studied transition between these global regulations, switch that correlates with positioning wrist, which articulates main segments. show HOX13 proteins themselves help off telomeric TAD,...
Osterix, a zinc finger transcription factor, is specifically expressed in osteoblasts and osteocytes of all developing bones. Because no bone formation occurs Osx-null mice, Osterix thought to be an essential regulator osteoblast differentiation. We report that, several mesenchymal osteoblastic cell types, BMP-2 induces increase expression the two isoforms arising from alternative promoters. identified consensus Sp1 sequence (GGGCGG) as binding regions fibromodulin sialoprotein promoters...
Significance Many genes important for vertebrate development are surrounded by multiple series of remote enhancer sequences. Such regulatory landscapes and their target usually located within the same chromatin domains, which appears to constrain action these sequences hence facilitate enhancer–promoter recognition gene expression. We used HoxD locus assess impact modifying topology upon activation in space time. A chromosomal rearrangements involving deletions inversions reveals that plays...
Abstract Osteoblast differentiation depends on the coordinated network of evolutionary conserved transcription factors during bone formation and homeostasis. Evidence indicates that morphogenetic protein (BMP) Wnt proteins regulate several steps skeletal development. Here, we provide a molecular description cooperative effects BMP canonical pathway expression early osteogenic genes Dlx5, Msx2, Runx2 in C2C12 cells, primary cultures marrow–mesenchymal stem organotypic calvarial cultures....
REVIEW article Front. Cell. Neurosci., 04 June 2013Sec. Cellular Neurophysiology Volume 7 - 2013 | https://doi.org/10.3389/fncel.2013.00087
Significance During vertebrate evolution, Hox gene function was coopted through the emergence of global enhancers outside clusters. Here, we analyze regulatory modalities underlying Hoxd transcription into developing mammary glands where proteins are necessary. We report existence a long-distance acting bud enhancer located near sequences involved in controlling genes limbs. argue that particular constitutive chromatin structure found at this locus facilitated element mammals by hijacking...
Bone formation and homeostasis is carried out by osteoblasts, whose differentiation activity are regulated osteogenic signaling networks. A central mediator of these inputs the lipid kinase phosphatidylinositol 3-kinase (PI3K). However, at present, there no data on specific role distinct class IA PI3K isoforms in bone biology. Here, we performed osteoblast-specific deletion mice to show that both p110α p110β required for survival function osteoblasts thereby control postnatal homeostasis....
Background p38 MAPK activity plays an important role in several steps of the osteoblast lineage progression through activation osteoblast-specific transcription factors and it is also essential for acquisition phenotype early development. Although reports indicate signalling a skeletal development, its specific contributions to adult bone remodelling are still be clarified. Methodology/Principal Findings We evaluated deletion p38α determine significance skeletogenesis, as well homeostasis...
DNA replication initiates at genomic loci known as origins, which fire according to a spatiotemporal program, still not yet well-defined in mammals. Here, we investigated whether pluripotent mouse embryonic stem cells (mESCs), proliferate rapidly and have very short G1 phases, different origin firing program than differentiated cells. Using EdU-seq combined with various cell cycle synchronization methods, identified initiation zones (IZs) mESCs, mesenchymal embryo fibroblasts (MEFs). Similar...
The spatial organization of the mammalian genome relies upon formation chromatin domains various scales. At level gene regulation in cis, collections enhancer sequences define large regulatory landscapes that usually match with presence topologically associating (TADs). These often contain ranges enhancers displaying similar or related tissue specificity, suggesting some cases, such may act as coherent units, a global on off state. By using HoxD cluster, which specifies topology developing...
During tetrapod limb development, the HOXA13 and HOXD13 transcription factors are critical for emergence organization of autopod, most distal aspect where digits will develop. Since previous work had suggested that Dbx2 gene is a target these factors, we set up to analyze in detail this potential regulatory interaction.We show HOX13 proteins bind mammalian-specific sequences at vicinity locus have enhancer activity developing digits. However, functional inactivation DBX2 protein did not...
Abstract Activation of p38 MAPK has been shown to be relevant for a number bone morphogenetic protein (BMP) physiological effects. We report here the involvement noncanonical phosphorylated mothers against decapentaplegic (Smad) signaling in transcriptional induction Cox2 (Ptgs2) by BMP-2 mesenchymal cells and organotypic calvarial cultures. demonstrate that different regulatory elements are required regulation expression BMP-2: Runt-related transcription factor-2 cAMP response element sites...
Mammalian genomes are partitioned into sub-megabase to megabase-sized units of preferential interactions called topologically associating domains or TADs, which likely important for the proper implementation gene regulatory processes. These provide structural scaffolds distant cis elements interact with their target genes within three-dimensional nuclear space and architectural proteins such as CTCF well cohesin complex participate in formation boundaries between them. However, importance...
The delivery of osteogenic factors is a proven therapeutic strategy to promote bone regeneration. Bone morphogenetic proteins (BMPs) constitute family cytokines with well-known and regenerative abilities. However, clinical uses BMPs require high doses that have been associated complications such as osteolysis, ectopic formation, or hematoma formation. In the present work, we sought improve tissue engineering through an approach combines use marrow-derived mesenchymal stem cells (BMMSCs),...
The skeleton acts as an endocrine organ that regulates energy metabolism and calcium phosphorous homeostasis through the secretion of osteocalcin (Oc) fibroblast growth factor 23 (FGF23). However, evidence suggests osteoblasts secrete additional unknown factors contribute to function bone. To search for these factors, we generated mice with a conditional osteoblast-specific deletion p38α MAPK known display profound defects in bone homeostasis. Herein, show impaired osteoblast is associated...
The phenolic compounds present in cocoa seeds have been studied regarding health benefits, such as antioxidant and anti-inflammatory activities. Fibrosis is a wound healing response that occurs almost all patients with chronic liver injury. A large number of cytokines soluble intercellular mediators are related to changes the behavior phenotype hepatic stellate cell (HSC) develop fibrogenic contractile leading development fibrosis. objective this study was assess catechin effect GRX cells...
ABSTRACT The mammalian HoxD cluster lies between two topologically associating domains (TADs) matching distinct, enhancer-rich regulatory landscapes. During limb development, the telomeric TAD controls early transcription of Hoxd gene in forearm cells, whereas centromeric subsequently regulates more posterior genes digit cells. Therefore, boundary prevents terminal Hoxd13 to respond enhancers, thereby allowing proper patterning. To assess nature and function this CTCF-rich DNA region embryo,...
ABSTRACT The HoxD gene cluster is critical for proper limb formation in tetrapods. In the emerging buds, different sub-groups of Hoxd genes respond first to a proximal regulatory signal, then distal signal that organizes digits. These two regulations are exclusive from one another and emanate distinct TADs flanking , both containing range appropriate enhancer sequences. telomeric TAD (T-DOM) contains several enhancers active presumptive forearm cells divided into sub-TADs separated by...
ABSTRACT Gene deserts are defined as genomic regions devoid of protein coding genes and spanning more than 500 kilobases, collectively encompassing about 25% the human genome. Approximately 30% all gene enriched for conserved elements with cis -regulatory signatures. These located predominantly near developmental transcription factors (TFs) but despite predicted critical functions, transcriptional contributions biological necessity most remain elusive. Here, we explore impact a desert...