A5027251317- Pleural and Pulmonary Diseases
- Protease and Inhibitor Mechanisms
- Occupational and environmental lung diseases
- Amoebic Infections and Treatments
- Enzyme Structure and Function
- Trauma Management and Diagnosis
- Monoclonal and Polyclonal Antibodies Research
- Biochemical and Molecular Research
- Streptococcal Infections and Treatments
- Respiratory Support and Mechanisms
- Cardiac Arrest and Resuscitation
- Blood Coagulation and Thrombosis Mechanisms
- Nosocomial Infections in ICU
- Burn Injury Management and Outcomes
- Venous Thromboembolism Diagnosis and Management
- Inhalation and Respiratory Drug Delivery
- RNA and protein synthesis mechanisms
- COVID-19 Clinical Research Studies
- Protein Structure and Dynamics
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pneumonia and Respiratory Infections
- Peptidase Inhibition and Analysis
- Sepsis Diagnosis and Treatment
- DNA and Nucleic Acid Chemistry
- Neutropenia and Cancer Infections
The University of Texas at Tyler
2020-2025
The University of Texas Health Science Center at Tyler
2016-2025
Kurchatov Institute
2025
Lung Institute
2018-2020
Portland State University
2007
Henry Ford Health System
2001-2004
University of Missouri
1995-2001
Harry S. Truman Memorial Veterans' Hospital
2001
Czech Academy of Sciences, Institute of Molecular Genetics
1998
Genetika
1994-1995
The increased levels of extracellular DNA found in a number disorders involving dysregulation the fibrinolytic system may affect interactions between enzymes and inhibitors. Double-stranded (ds) oligonucleotides bind tissue-(tPA) urokinase (uPA)-type plasminogen activators, plasmin, with submicromolar affinity. binding to was detected by EMSA, steady-state, stopped-flow fluorimetry. interaction dsDNA/oligonucleotides tPA uPA includes fast bimolecular step, followed two monomolecular steps,...
Local derangements of fibrin turnover and plasminogen activator inhibitor (PAI)-1 have been implicated in the pathogenesis pleural injury. However, their role control organization has unclear. We found that a C57Bl/6j mouse model carbon black/bleomycin (CBB) injury demonstrates resulting rind formation (14 d). In transgenic mice overexpressing human PAI-1, intrapleural deposition was increased, but visceral thickness, lung volumes, compliance were comparable to wild type. CBB PAI-1(-/-)...
Proprotein convertases (PCs) constitute an enzyme family that includes nine highly specific human subtilisin-like serine proteases. It is known the PCs mRNA levels vary in tumors, and these proteases are involved carcinogenesis. Thus, may be considered as potential markers for typing predicting course of disease, well targets therapy. We used quantitative real-time PCR to evaluate expression PC genes paired samples tumor adjacent normal tissues derived from 19 patients with esophageal...
Elevated concentrations of plasminogen activator inhibitor-1 (PAI-1) are associated with pleural injury, but its effects on organization remain unclear. A method adenovirus-mediated delivery genes interest (expressed under a cytomegalovirus promoter) to rabbit pleura was developed and used lacZ human (h) PAI-1. Histology, β-galactosidase staining, Western blotting, enzymatic immunohistochemical analyses fluids (PFs), lavages, mesothelial cells were evaluate the efficiency transduction....
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
The incidence of empyema (EMP) is increasing worldwide; EMP generally occurs with pleural loculation and impaired drainage often treated intrapleural fibrinolytic therapy (IPFT) or surgery. A number IPFT options are used clinically empiric dosing variable outcomes in adults. To evaluate mechanisms governing fibrinolysis disease outcomes, models Pasteurella multocida Streptococcus pneumoniae were generated rabbits the animals either human tissue (tPA) plasminogen activator prourokinase...
Current dosing of intrapleural fibrinolytic therapy (IPFT) in adults with complicated parapneumonic effusion (CPE) / empyema is empiric, as dose-escalation trials have not previously been conducted. We hypothesized that LTI-01 (scuPA), which relatively resistant to PA inhibitor-1 (PAI-1), would be well-tolerated.
Uridine phosphorylase from E. coli (Upase) has been crystallized using vapor diffusion technique in a new monoclinic crystal form. The structure was determined by the molecular replacement method at 2.5 Å resolution. coordinates of trigonal form were used as starting model and refinement program XPLOR led to R‐factor 18.6%. amino acid fold protein found be same that crystals. positions flexible regions refined. conclusion about involvement active site is good agreement with results...
The proenzyme single-chain urokinase plasminogen activator (scuPA) more effectively resolved intrapleural loculations in rabbits with tetracycline (TCN)-induced loculation than a range of clinical doses two-chain uPA (Abbokinase) and demonstrated trend toward greater efficacy tPA (Activase) (Idell S et al., Exp Lung Res 33: 419, 2007.). scuPA slowly generates durable fibrinolytic activity Abbokinase or Activase, but the interactions these agents inhibitors pleural fluids (PFs) have been...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Complicated pleural effusions and empyema with loculation failed drainage are common clinical problems. In adults, intrapleural fibrinolytic therapy is commonly used variable results remains empiric. Despite the use of various plasminogen activators; fibrinolysins, for about sixty years, there no clear consensus which agent most effective. Emerging evidence demonstrates that administration activators subject to rapid inhibition by activator inhibitor-1 processing fibrinolysins importantly...
Abstract Background Pleural infection affects about 65,000 patients annually in the US and UK. In this other forms of pleural injury, mesothelial cells (PMCs) undergo a process called (Meso) mesenchymal transition (MT), by which PMCs acquire profibrogenic phenotype with increased expression α‐smooth muscle actin (α‐SMA) matrix proteins. MesoMT thereby contributes to organization fibrosis lung restriction. Current murine empyema models are characterized early mortality, limiting analysis...
We have delineated two different reaction mechanisms of monoclonal antibodies (mAbs), MA-8H9D4 and either MA-55F4C12 or MA-33H1F7, that convert plasminogen activator inhibitor 1 (PAI-1) to a substrate for tissue (tPA)- urokinase activators. almost completely (98–99%) shifts the pathway by preventing disordering proteinase active site. does not affect rate-limiting constants (k lim) insertion reactive center loop cleaved tPA (3.5 s−1) but decreases k lim from 25 4.0 s−1. cause deacylation...