A5025893282- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- CAR-T cell therapy research
- Immune cells in cancer
- Skin and Cellular Biology Research
- Phagocytosis and Immune Regulation
- Cancer, Lipids, and Metabolism
- Marine Biology and Environmental Chemistry
- Cancer, Hypoxia, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Immune Cell Function and Interaction
University of Utah
2019-2025
Abstract Approximately half of melanoma patients relapse or fail to respond current standards care, highlighting the need for new treatment options. Engineering T-cells with chimeric antigen receptors (CARs) has revolutionized hematological malignancies but been clinically less effective in solid tumors. We therefore sought engineer alternative immune cell types inhibit progression. macrophages CARs emerged as a promising approach overcome some challenges faced by CAR-T cells; however,...
Focal adhesions are structures that physically link the cell to extracellular matrix for migration. Although culture studies have provided a wealth of information regarding focal adhesion biology, it is critical understand how dynamically regulated in their native environment. We developed zebrafish system visualize during single-cell migration vivo. find key site phosphoregulation (Y118) on Paxillin exhibits reduced phosphorylation migrating cells vivo compared vitro. Furthermore,...
ABSTRACT Chimeric antigen receptor (CAR) T-cell therapy has revolutionized the treatment of hematological malignancies but been clinically less effective in solid tumors. Engineering macrophages with CARs emerged as a promising approach to overcome some challenges faced by CAR-T cells due macrophage’s ability easily infiltrate tumors, phagocytose their targets, and reprogram immune response. We engineered CAR-macrophages (CAR-Ms) target chondroitin sulfate proteoglycan 4 (CSPG4), an...
Abstract While CAR T-cells have been a success in treating liquid tumors, these successes not replicated solid tumors. There are several reasons for challenges, including physical matrix barriers and increased tumor antigen heterogeneity Macrophages frequently found melanoma even those that low T-cell infiltrate, suggesting macrophages well equipped to penetrate the surrounding also antigen-presenting cells, thus, if phagocytose tumor, they will likely present additional antigens T-cells,...
Summary Focal adhesions are important subcellular structures that physically link the cell to extracellular matrix (ECM), thus facilitating efficient migration. Although in vitro culture studies have provided a wealth of information regarding focal adhesion biology, it is critical understand how dynamically regulated their native environment. We developed zebrafish transplantation system which we could efficiently visualize during single migration vivo with high-resolution live imaging. By...