A5100365769- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Mast cells and histamine
- Urticaria and Related Conditions
- Food Allergy and Anaphylaxis Research
- Immune Cell Function and Interaction
- Osteoarthritis Treatment and Mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Autoimmune Bullous Skin Diseases
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Soft tissue tumor case studies
- Allergic Rhinitis and Sensitization
- Immunodeficiency and Autoimmune Disorders
- RNA and protein synthesis mechanisms
- Chemokine receptors and signaling
- Connective tissue disorders research
- Eosinophilic Esophagitis
- Bone Tumor Diagnosis and Treatments
- Spondyloarthritis Studies and Treatments
- Anatomy and Medical Technology
- Colorectal Cancer Surgical Treatments
- Genital Health and Disease
- Glycosylation and Glycoproteins Research
- Sarcoma Diagnosis and Treatment
Affiliated Hospital of Hebei University
2024
Guilin University of Electronic Technology
2024
Zhejiang University
2023
Hangzhou Cancer Hospital
2023
Sun Yat-sen University
2022
Fifth Affiliated Hospital of Sun Yat-sen University
2022
University of California, Los Angeles
2001-2021
Second Xiangya Hospital of Central South University
2020-2021
Central South University
2020-2021
Children's Hospital of Chongqing Medical University
2019
Purpose: Patients with chronic spontaneous urticaria (CSU) have an increased risk for comorbid autoimmune diseases.In this retrospective multicenter study of CSU patients, we evaluated clinical and laboratory features associated a higher diseases.Methods: We analyzed records patients (n = 1,199) history or presence diseases.Patients were diagnosed type IIb (aiCSU) if all 3 tests positive: autologous serum skin test (ASST), basophil histamine release assay (BHRA) and/or activation (BAT), IgG...
ABSTRACT Background IgG autoantibodies against the high‐affinity IgE receptor, FcɛRIα, contribute pathogenesis of autoimmune chronic spontaneous urticaria (CSU). However, it is not known whether such patients also exhibit IgM or IgA FcɛRIα. To address this question we developed an ELISA to assess serum levels IgG, IgM, and FcɛRIα investigated their presence linked clinical features CSU including response autologous skin testing (ASST). Methods Serum samples 35 (25 ASST‐positive) 52 healthy...
Signaling through the high affinity IgE receptor FcϵRI on human basophils and rodent mast cells is decreased by co-aggregating these receptors to low IgG FcγRII. We used a recently described fusion protein, GE2, which composed of key portions γ1 ϵ heavy chains, dissect mechanisms that lead cell basophil inhibition co-aggregation FcγRII FcϵRI. Unstimulated derived from umbilical cord blood express immunoreceptor tyrosine-based inhibitory motif-containing but not FcγRI or FcγRIII. Interaction...
Abstract Activation-induced cytidine deaminase (AID) plays critical roles in Ig class switch recombination and VH gene somatic hypermutation. We investigated the role of IL-4 AID mRNA induction, signaling transduction involved IL-4-mediated effect CD45 on IL-4-dependent expression human B cells. was able to induce primary cells cell lines, IL-4-induced further enhanced by CD40 signaling. induction inhibited a dominant-negative STAT6, indicating that induced via Janus kinase (JAK)/STAT6...
We have identified a novel chordin-like protein, CHL2, which is structurally most homologous to CHL/neuralin/ventroptin. When injected into Xenopus embryos, CHL2 RNA induced secondary axis. Recombinant protein interacted directly with BMPs in competitive manner prevent binding the type I BMP receptor ectodomain, and inhibited BMP-dependent induction of alkaline phosphatase C2C12 cells. Thus, behaves as secreted BMP-binding inhibitor. In situ hybridization revealed that expression restricted...
Chondrogenic potential of human adipose derived stem cells (hASCs) makes them a possible source seeding for cartilage tissue engineering. We aim to examine the chondrogenic differentiation transforming growth factor beta2 (hTGF beta2) transduced hASCs seeded in three-dimensional scaffold vitro and vivo. In this study, were isolated from subcutaneous with replication deficient adenovirus carrying hTGF (Ad5-hTGF beta2), then cultured PLGA/alginate compounds. RT-PCR analysis revealed that...
Activation-induced cytidine deaminase (AID) initiates somatic hypermutation and class switch recombination in B cells by deaminating C → U on transcribed DNA. Here we analyze the role of phosphorylation phosphorylation-null mutants biochemical behavior AID, including enzyme specific activity, processivity, deamination spectra, motif specificity, transcription-dependent presence absence RPA. We show that a small fraction recombinant human AID expressed Sf9 insect is phosphorylated at...
Abstract To compare the efficacy of curettage and bone grafting combined with elastic intramedullary nailing (EIN) vs in treatment long cysts children to clarify necessity using EIN cysts. Sixty-two patients were involved this study from Jan. 2009 Sept. 2017 (43 males, 19 females; 27 humeri, 35 femurs); assigned an group, comprising 30 who underwent EIN, or a non–elastic (NEIN) 32 alone. The prognosis 2 groups was assessed reference standard Capanna. No statistically significant differences...
Four human IgE isoforms produced by alternative splicing of the epsilon primary transcript were expressed as chimeric mouse/human anti 5-dimethylamino-1-naphthalenesulfonyl antibodies in murine myeloma cell line Sp2/0. The four include classic secreted form and three novel with altered carboxyl termini. All these lack transmembrane region encoded M1/M1′ exon are therefore predicted to be proteins. When Sp2/0 cells, assembled into complete molecules two Ig heavy chains light chains, whereas...
Backgrounds Advanced ovarian cancer is frequently accompanied by extensive peritoneal metastasis, complicating surgical interventions. This study aims to explore the application of 3D reconstruction and printing technology in treatment advanced cancer. Methods We conducted a retrospective analysis 60 patients with stage III who underwent cytoreductive surgery at Hebei University Affiliated Hospital between 2020 2023. Patients were randomly assigned three groups: visualization group, plus...
To the Editor: Solitary fibrous tumors (SFTs) are mesenchymal neoplasms and were first described in pleura, then subsequently found extrapleural organs, such as peritoneum, lung, parotid gland, paranasal sinuses, orbit, skin, intracranial areas.[1] However, SFTs limited to isolated case reports. Because of rarity them, related information is far from sufficient thus, leading this tumor under fully recognized. According World Health Organization (WHO) classification, pluripotent that could be...
Abstract Allergic disorders have now become a major worldwide public health issue, but the effective treatment options remain limited. We report novel approach to block allergic reactivity by targeting surface-bound IgE of effector cells via low-affinity anti-human Abs with dissociation constants in 10−6 10−8 M range. demonstrated that these anti-IgE mAbs bind cell surface–bound without triggering anaphylactic degranulation even at high concentration, albeit they would weakly upregulate...
To evaluate the diagnostic value of quantitative parameters [T1, T2, and proton density (PD) value] generated from magnetic resonance image compilation (MAGiC) sequence for active sacroiliitis in patients with axial spondyloarthritis (ax-SpA).A total 90 consecutive ax-SpA were recruited divided into an group (n=48) inactive (n=42) based on Spondyloarthritis Research Consortium Canada (SPARCC) score this prospective study. In addition, 47 healthy volunteers as control group. All participants...
One key unresolved issue in immunoglobulin class switch recombination (CSR) is how the accessibility of region for CSR controlled. To better understand nature control human Ig CSR, we developed a novel inducible assay based on expression green fluorescence protein (GFP) from constructs undergoing substrate (SSR). Efficient SSR depends cytokine-inducible Iϵ promoter and co-stimulation with IL-4+anti-CD40. Characterization reveals that both S-S deletional S-Sinversion occur. We show...
A splicing mutation in VPS4B can cause dentin dysplasia type I (DD-I), a hereditary autosomal-dominant disorder characterized by rootless teeth, the etiology of which is genetically heterogeneous. In our study, dental follicle cells (DFCs) were isolated and cultured from patient with DD-I compared those an age-matched, healthy control. previous this was confirmed to have loss-of-function (IVS7 + 46C > G). The results study showed that DFCs vimentin-positive CK14-negative, indicating derived...