A5008930612- Virus-based gene therapy research
- Cellular Mechanics and Interactions
- Nuclear Structure and Function
- Retinal Development and Disorders
- 3D Printing in Biomedical Research
- Mitochondrial Function and Pathology
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Parvovirus B19 Infection Studies
- Animal Virus Infections Studies
- Adipose Tissue and Metabolism
- Connexins and lens biology
- Advanced Fluorescence Microscopy Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Force Microscopy Techniques and Applications
- RNA Interference and Gene Delivery
- Advanced Materials and Mechanics
- Microfluidic and Bio-sensing Technologies
- Physiological and biochemical adaptations
- Bacteriophages and microbial interactions
- Luminescence and Fluorescent Materials
- Cell Adhesion Molecules Research
- Protein Structure and Dynamics
Tampere University
2016-2025
La Trobe University
2023
Fimlab (Finland)
2017
ETH Zurich
2006-2015
University of Jyväskylä
2005-2013
Center for NanoScience
2007
The cell-penetrating peptide derived from the Human immunodeficiency virus-1 transactivator protein Tat possesses capacity to promote effective uptake of various cargo molecules across plasma membrane in vitro and vivo. objective this study was characterize delivery mechanisms a novel streptavidin fusion construct, TAT47-57-streptavidin (TAT-SA, 60 kD). SA represents potentially useful TAT-fusion partner due its ability perform as versatile intracellular vector for wide array biotinylated or...
We introduce a new method for mesoscopic modeling of protein diffusion in an entire cell. This is based on the construction three-dimensional digital model cell from confocal microscopy data. The segmented into cytoplasm, nucleus, plasma membrane, and nuclear envelope, which environment motion modeled by fully numerical methods. Finer cellular structures that cannot be resolved with imaging technique, significantly affect motion, are accounted this assigning effective, position-dependent...
Cellular immune responses require the generation and recruitment of diverse blood cell types that recognize kill pathogens. In Drosophila melanogaster larvae, immune-inducible lamellocytes participate in recognizing killing parasitoid wasp eggs. However, sequence events required for lamellocyte remains controversial. To study cellular system, we developed a flow cytometry approach using vivo reporters as well plasmatocytes, main hemocyte type healthy larvae. We found two different lineages,...
Microstructure plays an essential role in the control of hydrogel properties. It is also important factor when cells or drugs are encapsulated inside hydrogel. In this work, microstructures hydrazone crosslinked hyaluronan-, alginate- and gellan gum-based hydrogels were evaluated thoroughly for first time by using rheology- diffusion (fluorescence recovery after photobleaching, FRAP)-based methods. The effect gel parameters on viscoelastic properties hydrogels, further their structural (mesh...
Neural tissue engineering and three-dimensional in vitro modeling require the development of biomaterials that take into account specified requirements human neural cells tissue. In this study, an alternative method producing biomimetic hydrogels based on gellan gum (GG) was developed by replacing traditional crosslinking methods with bioamines spermidine spermine. These were proven to function as crosslinkers for GG hydrogel at +37 °C, allowing encapsulation neurons. We studied mechanical...
Abstract Nuclear lamins have been considered an important structural element of the nucleus. The nuclear lamina is thought both to shield DNA from excessive mechanical forces and transmit onto DNA. However, date there not yet a technical approach directly measure on at protein level. To overcome this limitation, we developed nanobody-based intermolecular tension FRET biosensor capable measuring strain lamin filaments. Using sensor, were able show that subjected significant force. These are...
Cells adhere to the surrounding tissue and probe its mechanical properties by forming cell-matrix adhesions. Talin is a critical adhesion protein participates in transmission of signals between extracellular matrix cell cytoskeleton. Force induced unfolding talin rod subdomains has been proposed act as cellular mechanosensor, but so far evidence linking their stability response lacking. Here, utilizing computationally designed mutations, we demonstrate that stepwise destabilization R3...
Dielectrophoresis can potentially be used as an efficient trapping tool in the fabrication of molecular devices. For nanoscale objects, however, Brownian motion poses a challenge. We show that use carbon nanotube electrodes makes it possible to apply relatively low voltages and still achieve high enough field gradients for e.g., single molecules. compare efficiency other characteristics dielectrophoresis between lithographically fabricated metallic electrodes, case DNA The results are...
The success of synthetic bone implants requires good interface between the material and host tissue. To study biological relevance fibronectin (FN) density on osteogenic commitment human marrow mesenchymal stem cells (hBM-MSCs), FN was adsorbed in a linear gradient surface PCL. evolution markers alkaline phosphatase collagen 1 alpha monitored by immunohistochemistry, cytoskeletal organization cell-derived were assessed. functional analysis revealed that lower FN-density elicited stronger...
Abstract Surface topography is a key parameter in regulating the morphology and behavior of single cells. At multicellular level, coordinated cell displacements drive many biological events such as embryonic morphogenesis. However, effect surface on collective migration epithelium has not been studied detail. Mastering connection between features cellular behaviour highly important for novel approaches tissue engineering repair. Herein, we used photopatterned microtopographies...
Based on studies with a fluorescent reporter dye, Mito Thermo Yellow (MTY), and the genetically encoded gTEMP ratiometric temperature indicator targeted to mitochondria, of active mitochondria in four mammalian one insect cell line was estimated be up 15°C above that external environment which cells were exposed. High mitochondrial maintained face variety metabolic stresses, including substrate starvation or modification, decreased ATP demand due inhibition cytosolic protein synthesis,...
The dielectrophoresis method for trapping and attaching nanoscale double-stranded DNA between nanoelectrodes was developed. gives a high yield of single or few molecules only which enables transport measurements at the molecule level. Electrical conductivity individual 140-nm-long measured, showing insulating behavior in dry conditions. In contrast, clear enhancement observed moist conditions, relating to interplay conformation their conductivity.
As the complex structure of nervous tissue cannot be mimicked in two-dimensional (2D) cultures, development three-dimensional (3D) neuronal cell culture platforms is a topical issue field neuroscience and neural engineering. Computer-assisted laser-based fabrication techniques such as direct laser writing by two-photon polymerization (2PP-DLW) offer versatile tool to fabricate 3D with highly ordered geometries size scale natural environments. In this study, we present design 2PP-DLW process...
Tight junctions are dynamic structures that crucial in establishing the diffusion and electrical barrier of epithelial monolayers. Dysfunctions tight can impede this function lead to many pathological conditions. Unfortunately, detailed understanding non-specific permeation pathway through junctions, so-called leak pathway, is lacking. We created computational models describe two main measures, molecular permeability transepithelial electric resistance while using common structural dynamics....
Autonomous parvoviruses encode at least two nonstructural proteins, NS1 and NS2. While is linked to important nuclear processes required for viral replication, much less known about the role of Specifically, function canine parvovirus (CPV) NS2 has remained undefined. Here we have used proximity-dependent biotin identification (BioID) screen proteins that associate with CPV Many these associations were seen both in noninfected infected cells, however, major type interacting shifted from...
Based on studies with a fluorescent reporter dye, Mito Thermo Yellow (MTY), and the genetically encoded gTEMP ratiometric temperature indicator targeted to mitochondria, of active mitochondria in four mammalian one insect cell line was estimated be up 15°C above that external environment which cells were exposed. High mitochondrial maintained face variety metabolic stresses, including substrate starvation or modification, decreased ATP demand due inhibition cytosolic protein synthesis,...
Mechanical properties of the nucleus are remodeled not only by extracellular forces transmitted to but also internal modifications, such as those induced viral infections. During herpes simplex virus type 1 infection, regulation essential nuclear functions and growth replication compartments known reorganize structures. However, little is about how this infection-induced deformation changes mechanobiology. Our analyses showed that softens during infection. To understand why happens, we used...
Retinal pigment epithelium (RPE) is an epithelial monolayer located at the back of eye. RPE and retina interact closely, with performing multiple tasks ensuring proper retinal physiology forming a tight, interlocked structure photoreceptor outer segments (POS). Especially, participates in essential daily renewal POS phagocytosis process. However, current vitro cell culture systems lack contact between retina, possibly limiting not only maturation but also hindering studies addressing...
Switchavidin is a chicken avidin mutant displaying reversible binding to biotin, an improved affinity toward conjugated and low nonspecific due reduced surface charge. These properties make switchavidin optimal tool in biosensor applications for the immobilization of biotinylated proteins on sensor surfaces. Furthermore, opens novel possibilities patterning, purification, labeling.
Phagocytosis of retinal rod and cone outer segment (OS) tips by the pigment epithelium (RPE) occurs daily to prevent accumulation harmful compounds in photoreceptors. Rhythmic bursts phagocytosis, seen as increased levels phagocytosed OS particles RPE, are known appear once or twice a day depending on animal species. However, differences rhythmicity phagocytosis between distinct photoreceptor types not well understood. Here, we show that subtype OSs does have identical rhythmic profiles...
ABSTRACT The replication protein NS1 is essential for genome and production in parvoviral infection. Many of its functions, including recognition site-specific nicking the viral genome, helicase activity, transactivation capsid promoter, are dependent on ATP. An ATP-binding pocket resides middle modular a superfamily 3 domain. Here we have identified key amino acid residues canine parvovirus (CPV) mutated acids from conserved A motif (K406), B (E444 E445), positively charged region (R508...