- Environmental Toxicology and Ecotoxicology
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Aquaculture disease management and microbiota
- Marine Bivalve and Aquaculture Studies
- Protein Hydrolysis and Bioactive Peptides
- Health, Environment, Cognitive Aging
- Microbial bioremediation and biosurfactants
- Marine Biology and Environmental Chemistry
- Microplastics and Plastic Pollution
- Transportation Planning and Optimization
- Aquaculture Nutrition and Growth
- Invertebrate Immune Response Mechanisms
- Vehicle emissions and performance
- Effects and risks of endocrine disrupting chemicals
- Air Quality and Health Impacts
- Identification and Quantification in Food
Stockholm University
2006-2015
University of the Basque Country
2007
Uppsala University
2007
Bivalves and especially mussels are very good indicators of marine estuarine pollution, so they have been widely used in biomonitoring programs all around the world. However, traditional single parameter biomarkers face problem high sensitivity to biotic abiotic factors. In our study, digestive gland peroxisome-enriched fractions Mytilus edulis (L., 1758) were analyzed by DIGE MS. We identified several proteomic signatures associated with exposure pollutants (diallyl phthalate, PBDE-47,...
Using a proteomic approach, we have developed new method for the assessment of marine pollution that generates highly reproducible protein expression patterns and it is simple scalable. The protocol based on applying liquid chromatography (LC) coupled with two-dimensional electrophoresis (2-DE) to analyze changes in pattern after exposure pollution. digestive gland sentinel "blue mussel" (Mytilus edulis) was batch-processed through cell fractionation followed by ion-exchange 2-DE. selection...
The marine mussel innate immunity provides protection to pathogen invasion and inflammation. In this regard, the hemolymph takes a main role in animal response. Despite importance of body fluid determining physiological condition animal, little is known about molecular mechanisms underlying cellular humoral responses. work, we have applied MS (nano-LC-MS/MS) strategy integrating genomic transcriptomic data with aim to: (i) identify protein functional groups that characterize (ii) map...
Peroxisomal proteomic protein profiles of exposure to marine pollution have been recently introduced in biomonitoring experiments. However, laboratory experiments study the independent effect common pollutants are needed define a minimal expression signature (PES) specific pollutant. The aim this was obtain PESs blue mussels (Mytilus edulis) exposed two different crude oil mixtures for future application areas affected by spills. In study, peroxisome-enriched fractions from digestive gland...
Biomonitoring programs that use mussels to assess t he water quality around the world could benefit fro m of proteomics techniques. These be applie d obtain protein expression signatures exposu re pollution further used for prediction purposes. This would require a combination univariate and multivariate statistical analyses data were utilized robust models. We sho w an appli- cation this approach on exposed fresh fuel, weathered fuel in laboratory experimen tried mimic effects Prestige ’s...
This Paper presents the Study recently carried out by LABEIN for Spanish Administration (Ministry of Works, Transport and Environment), which objectives have been following: to identify, define quantify main variables responsible emissions nitrogen oxides (NOJ organic volatile compounds (VOC) from urban traffic develop guidelines a strategic plan minimization control these emissions. To carry first part study, two measurement campaigns were out: one them inside tunnel other in ambient. On...