A5078776822- Microbial Metabolites in Food Biotechnology
- Antifungal resistance and susceptibility
- Environmental remediation with nanomaterials
- Heavy metals in environment
- SARS-CoV-2 and COVID-19 Research
- Food composition and properties
- Animal Virus Infections Studies
- Research on Leishmaniasis Studies
- Chromium effects and bioremediation
- Polysaccharides and Plant Cell Walls
- Bioinformatics and Genomic Networks
- Amino Acid Enzymes and Metabolism
- Parathyroid Disorders and Treatments
- Nanoparticles: synthesis and applications
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
- SARS-CoV-2 detection and testing
- Endoplasmic Reticulum Stress and Disease
- Clostridium difficile and Clostridium perfringens research
- Fungal and yeast genetics research
- Dialysis and Renal Disease Management
- Gene expression and cancer classification
- Chronic Kidney Disease and Diabetes
- Microbial bioremediation and biosurfactants
- Biofuel production and bioconversion
Universidad Complutense de Madrid
2010-2024
In this study, we describe SARS-CoV-2 infection dynamics in one cat and three dogs from households with confirmed human cases of COVID-19 living the Madrid Community (Spain) at time expansion (December 2020 through June 2021) alpha variant (lineage B.1.1.7). A thorough physical exam nasopharyngeal, oropharyngeal, rectal swabs were collected for real-time reverse-transcription PCR (RT-qPCR) testing on day 0 successive samplings days 7, 14, 21, 47 during monitoring. Blood was also drawn to...
O-mannosylation is a crucial protein modification in eukaryotes that initiated by the essential family of O-mannosyltransferases (PMTs). Here we demonstrate model yeast Saccharomyces cerevisiae rhodanine-3-acetic acid derivatives affect members all PMT subfamilies. Specifically, used OGT2468 to analyse genome-wide transcriptional changes response general inhibition baker's yeast. results activation cell wall integrity (CWI) pathway. Coinciding, mitogen-activated kinase Slt2p activated vivo...
Abstract Peritoneal dialysis (PD) is an effective renal replacement therapy, but a significant proportion of patients suffer PD-related complications, which limit the treatment duration. Mesothelial-to-mesenchymal transition (MMT) contributes to peritoneal dysfunction. We analyzed genetic reprograming MMT identify new biomarkers that may be tested in PD-patients. Microarray analysis revealed partial overlapping between induced vitro and ex vivo effluent-derived mesothelial cells, mainly...
Nanoscale zero-valent iron (nZVI) is a strong reducing agent used for in situ remediation of soil. The impacts nZVI (5–10% w/w) on the soil microbial biodiversity and functionality two soils (Lufa 2.2 2.4) were assessed. Illumina MiSeq technology was to evaluate structure microbiomes after 21 days exposure. Proteobacteria, Verrucomicrobia, Firmicutes Actinobacteria most abundant phyla both soils. However, dynamics bacterial community composition following addition differed. exposure induced...
The enormous amount of data available in public gene expression repositories such as Gene Expression Omnibus (GEO) offers an inestimable resource to explore programs across several organisms and conditions. This information can be used discover experiments that induce similar or opposite patterns a given query, which turn may lead the discovery new relationships among diseases, drugs pathways, well generation hypotheses. In this work, we present MARQ, web-based application allows researchers...
The synthetic bacterial prionoid RepA-WH1 causes a vertically transmissible amyloid proteinopathy in Escherichia coli that inhibits growth and eventually kills the cells. Recent vitro studies show builds pores through model lipid membranes, suggesting possible mechanism for cell death. By comparing acutely (A31V) mildly (ΔN37) cytotoxic mutant variants of protein, we report here RepA-WH1(A31V) expression decreases intracellular osmotic pressure compromise viability under either aerobic or...
The cell wall is essential for the yeast to hypha (Y-H) transition that enables Candida albicans invade human tissues and evade immune system. main constituent, β(1,3)-glucan, remodeled by glucanosyltransferases of GH72 family. Phr1p responsible glucan remodeling at neutral-alkaline pH morphogenesis virulence. Due pH-regulated expression PHR1, phr1Δ phenotype manifested > 6 its severity increases with rise in pH. We exploited pH-conditional nature a PHR1 null mutant analyze impact on hyphal...
Visceral leishmaniasis (VL) is the most severe form of all leishmanial infections and caused by infection with protozoa Leishmania donovani infantum. This parasitic disease occurs in over 80 countries its geographic distribution on rise. Although interaction between intestinal microbiome immune response has been established several pathologies, it not widely studied leishmaniasis. The Syrian hamster advanced laboratory model for developing vaccines new drugs against VL. In study reported...
Visceral leishmaniasis (VL), a vector-borne parasitic disease caused by Leishmania donovani and L. infantum (Kinetoplastida), affects humans dogs, being fatal unless treated. Miltefosine (MIL) is the only oral medication for VL considered first choice drug when resistance to antimonials present. Comorbidity comedication are common in many affected patients but relationship between microbiome composition, drugs administered their pharmacology still unknown. To explore effect of clindamycin on...
In this study, we describe SARS-CoV-2 infection dynamics in one cat (case 1-Chester) and three dogs 2-Trasto, case 3-Bella 4-Bull) from households with confirmed human cases of COVID-19 living the Madrid Community (Spain) at time expansion (December 2020 through June 2021) alpha variant (lineage B.1.1.7). A thorough physical exam nasopharyngeal, oropharyngeal, rectal swabs were collected for real-time reverse-transcription PCR (RT-qPCR) testing on day 0 successive samplings days 7, 14, 21 47...