A5026182688- Immune cells in cancer
- Autophagy in Disease and Therapy
- Immunotherapy and Immune Responses
- Retinal Diseases and Treatments
- Immune Response and Inflammation
- Nuclear Receptors and Signaling
- GDF15 and Related Biomarkers
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Connexins and lens biology
- Retinal Development and Disorders
- Cancer, Hypoxia, and Metabolism
- Probiotics and Fermented Foods
- T-cell and B-cell Immunology
- Retinopathy of Prematurity Studies
- Tryptophan and brain disorders
- Retinal Imaging and Analysis
- TGF-β signaling in diseases
- RNA modifications and cancer
- interferon and immune responses
- Diabetes and associated disorders
- HIV Research and Treatment
- Cytokine Signaling Pathways and Interactions
- Endoplasmic Reticulum Stress and Disease
- Mycobacterium research and diagnosis
Centro de Investigaciones Biológicas Margarita Salas
2010-2025
Consejo Superior de Investigaciones Científicas
2006-2018
Complejo Hospitalario Universitario de Toledo
2009
Hospital General Universitario Gregorio Marañón
2009
University of Toledo
2009
Instituto de Parasitología y Biomedicina "López - Neyra"
2006
Centro Nacional de Biotecnología
2006
Abstract The CCL2 chemokine mediates monocyte egress from bone marrow and recruitment into inflamed tissues through interaction with the CCR2 receptor, its expression is upregulated by proinflammatory cytokines. Analysis of gene profile in GM-CSF– M-CSF–polarized macrophages revealed that a high characterizes generated under influence M-CSF, whereas expressed only GM-CSF–polarized macrophages. factors responsible for this differential identified activin A as critical factor controlling...
Macrophage activation comprises a continuum of functional states critically determined by cytokine microenvironment. Activated macrophages have been functionally grouped according to their response pro-Th1/proinflammatory stimuli [lipopolysaccharide, IFNgamma, granulocyte macrophage colony-stimulating factor (GM-CSF); M1] or pro-Th2/anti-inflammatory [interleukin (IL)-4, IL-10, M-CSF; M2]. We report that folate receptor beta (FRbeta), encoded the FOLR2 gene, is marker for generated in...
Besides its role as a neurotransmitter, serotonin (5-hydroxytryptamine, 5HT) regulates inflammation and tissue repair via set of receptors (5HT(1-7)) whose pattern expression varies among cell lineages. Considering the importance macrophage polarization plasticity for inflammatory responses repair, we evaluated whether 5HT modulates human polarization. inhibited LPS-induced release proinflammatory cytokines without affecting IL-10 production, upregulated M2 polarization-associated genes...
The shift between pro-inflammatory (M1) and anti-inflammatory (M2) states of macrophage polarization allows the resolution inflammatory processes as well maintenance a basal environment in tissues continuously exposed to harmless antigens (e.g., lung gut). To identify markers for state macrophages, expression profiling was performed on human macrophages polarized by either GM-CSF or M-CSF, which lead generation TNF-alpha IL-12p40-producing [M1 (GM-CSF)] IL-10-producing [M2 (M-CSF)] upon...
Dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN; CD209) is a human pathogen-attachment C-type lectin with no obvious murine ortholog and for which ligation leads to enhanced anti-inflammatory cytokine release altered proinflammatory production. Although induced by IL-4 in monocytes considered as DC marker, DC-SIGN expression on APCs under homeostatic conditions so far unexplained. We report this study that M-CSF enhances vitro derived macrophages mediates the induction of...
Abstract In the last years, autophagy has been revealed as an essential pathway for multiple biological processes and physiological functions. As a catabolic route, regulation by nutrient availability evolutionarily conserved from yeast to mammals. On one hand, induction starvation is associated with significant loss in body weight mice. Here, we demonstrate that both genetic pharmacological inhibition of process compromise induced starvation. Moreover, autophagic potential also impacts on...
Exopolysaccharides have prebiotic potential and contribute to the rheology texture of fermented foods. Here we analyzed in vitro bioavailability immunomodulatory properties 2-substituted (1,3)-beta-D-glucan-producing bacterium Pediococcus parvulus 2.6. It resists gastrointestinal stress, adheres Caco-2 cells, induces production inflammation-related cytokines by polarized macrophages.
Abstract Modulation of macrophage polarization underlies the onset and resolution inflammatory processes, with polarization-specific molecules being actively sought as potential diagnostic therapeutic tools. Based on their cytokine profile upon exposure to pathogenic stimuli, human monocyte-derived macrophages generated in presence GM-CSF or M-CSF are considered proinflammatory (M1) anti-inflammatory (M2) macrophages, respectively. We report this study that prolyl hydroxylase PHD3-encoding...
Glaucoma is a common age-related disease leading to progressive retinal ganglion cell (RGC) death, visual field defects and vision loss the second cause of blindness in elderly worldwide. Mitochondrial dysfunction impaired autophagy have been linked glaucoma induction shows neuroprotective effects animal models. We shown that decreases with aging retina can be for RGCs, but it currently unknown how deficiency impact RGCs susceptibility survival. Using optic nerve crush model young...
The myeloid C-type lectin dendritic cell-specific ICAM3-grabbing non-integrin (DC-SIGN, CD209) recognizes oligosaccharide ligands on clinically relevant pathogens (HIV, Mycobacterium, and Aspergillus). Alternative splicing genomic polymorphism generate DC-SIGN mRNA variants, which have been detected at sites of pathogen entrance transmission. We present evidence that neck variants are expressed cells the RNA protein levels. Structural analysis revealed multimerization within a cellular...
Mitophagy is the process by which cells eliminate damaged or superfluous mitochondria degrading them within lysosomes. We show that during development, selective removal of autophagy induces a metabolic shift toward glycolysis essential for differentiation several cell types. These findings suggest potential applications cell-fate manipulation strategies targeting mitophagy.
Mitochondrial damage plays a prominent role in glaucoma. The only way cells can degrade whole mitochondria is via autophagy, process called mitophagy. Thus, studying mitophagy the context of glaucoma essential to understand disease. Up date limited tools are available for analyzing vivo. We have taken advantage mito-QC reporter, recently generated mouse model that allows an accurate assessment fill this gap. used primary RGCs and retinal explants derived from mice quantify activation vitro...
Abstract Photoreceptor degeneration is the hallmark of retinitis pigmentosa. Identifying general mechanisms underlying photoreceptor cell death key to developing effective, mutation-independent treatments prevent vision loss. Mitophagy a protective pathway that prevents age-dependent loss and upregulated by iron chelators such as deferiprone (DFP). Therefore, we aimed investigate ability DFP protect against retinal via mitophagy. First, treated mitophagy reporter mice with MNU, classic...
GM-CSF promotes the functional maturation of lung alveolar macrophages, whose differentiation is dependent on PPARγ transcription factor. In fact, blockade GM-CSF-initiated signaling or deletion PPARγ-encoding gene PPARG leads to functionally defective macrophages and onset pulmonary proteinosis. vitro, generated in presence display potent pro-inflammatory, immunogenic tumor growth-limiting activities. Since upregulates expression, we hypothesized that might contribute signature profile...
Abstract Vascular smooth muscle cell (VSMC) proliferation is essential for arteriogenesis to restore blood flow after artery occlusion, but the mechanisms underlying this response remain unclear. Based on our previous findings showing increased VSMC in neonatal aorta of mice lacking protease MT4-MMP, we aimed at discovering new players process. We demonstrate that MT4-MMP absence boosted vitro PDGF-BB a cell-autonomous manner through enhanced p38 MAPK activity. Increased phospho-p38 basal...
Macroautophagy/autophagy is a key process in the maintenance of cellular homeostasis. The age-dependent decline retinal autophagy has been associated with photoreceptor degeneration. Retinal dysfunction can also result from damage to pigment epithelium (RPE), as RPE-retina constitutes an important metabolic ecosystem that must be finely tuned preserve visual function. While studies mice lacking essential genes have revealed predisposition degeneration, consequences moderate reduction...
Abstract CD36 is a member of the scavenger receptor type B family implicated in binding lipoproteins, phosphatidylserine, thrombospondin-1, and uptake long-chain fatty acids. On mononuclear phagocytes, recognition apoptotic cells by contributes to peripheral tolerance prevention autoimmunity impairing dendritic cell (DC) maturation. Besides, acts as coreceptor with TLR2/6 for sensing microbial diacylglycerides, its deficiency leads increased susceptibility Staphylococcus aureus infections....
Excessive energy management leads to low-grade, chronic inflammation, which is a significant factor predicting noncommunicable diseases. In turn, oxidation, and metabolism are associated with the course of these diseases; mitochondrial dysfunction seems be at crossroads mutual relationships. The migration immune cells during inflammation governed by interaction between chemokines chemokine receptors. Chemokines, especially C-C-chemokine ligand 2 (CCL2), have variety additional functions that...