A5001157533- Environmental DNA in Biodiversity Studies
- Identification and Quantification in Food
- Marine Ecology and Invasive Species
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Endoplasmic Reticulum Stress and Disease
- Protist diversity and phylogeny
- Fungal and yeast genetics research
University of Minho
2016-2024
Marine Research Centre
2021
Benthic macroinvertebrates are among the most used biological quality elements for assessing condition of all types aquatic ecosystems worldwide (i.e., fresh water, transitional, and marine). Current morphology-based assessments have several limitations that may be circumvented by using DNA-based approaches. Here, we present a comprehensive review 90 publications on use DNA metabarcoding benthic in bioassessments. Metabarcoding bulk macrozoobenthos has been preferentially waters, whereas...
DNA metabarcoding provides a rapid and effective identification tool of macroinvertebrate species. The accuracy species-level assignment, consequent taxonomic coverage, relies on comprehensive barcode reference libraries, which, due to incompleteness, are currently recognized limitation for applications. In this study, we assembled library barcodes Atlantic Iberia marine species, assessed gaps in species coverage examined data ambiguities. Initially, an Iberian checklist the three dominant...
Marine hard-bottom communities are undergoing severe change under the influence of multiple drivers, notably climate change, extraction natural resources, pollution and eutrophication, habitat degradation, invasive species. Monitoring marine biodiversity in such habitats is, however, challenging as it typically involves expensive, non-standardized, often destructive sampling methods that limit its scalability. Differences monitoring approaches furthermore hinders inter-comparison among...
DNA metabarcoding has great potential to improve marine biomonitoring programs by providing a rapid and accurate assessment of species composition in zoobenthic communities. However, some methodological improvements are still required, especially regarding failed detections, primers efficiency incompleteness databases. Here we assessed the two different marker loci (COI 18S) three primer pairs detection through macrozoobenthic communities colonizing types artificial substrates (slate, PVC...
In marine and estuarine benthic communities, the inventory estimation of species richness are often hampered by need for broad taxonomic expertise across several phyla. The use DNA metabarcoding has emerged as a powerful tool fast assessment composition in diversity ecological communities. Here, we tested amplification success five primer sets targeting different COI-5P regions 454 pyrosequencing to maximize recovery two simulated macrobenthic communities containing 21 (SimCom1 SimCom 2)....
We used DNA metabarcoding to compare macrozoobenthic species colonization between autonomous reef monitoring structures (ARMS) and artificial seaweed systems (ASMS). deployed both substrates in two different locations (Ría de Vigo Ría Ferrol, NW Iberian coast) collected them after 6, 9, 12 months assess composition of the colonizing communities through high-throughput sequencing amplicons within barcode region mitochondrial cytochrome c oxidase I (COI-5P) V4 domain 18S rRNA genes. observed a...
Abstract In batch cultures of Saccharomyces cerevisiae grown on lactate, medium alkalinization coincides with endocytosis the Jen1 lactate/pyruvate transporter. To assess how culture pH impacts endocytosis, S. was in carbon-limited continuous a mixed ethanol-lactate feed. When applying linearly increasing (6.75 to 7.25), extracellular lactate and pyruvate concentrations increased progressively. Up 7.0, these aligned thermodynamic equilibrium Jen1-mediated electroneutral carboxylate-proton...
Event Abstract Back to Combining artificial substrates, morphology and DNA metabarcoding for investigating macrozoobenthic communities in NW Iberia Barbara R. Leite1, 2, 3*, Pedro E. Vieira1, Jesus S. Troncoso3, 4 Filipe O. Costa1, 2 1 Centre of Molecular Environmental Biology, Department University Minho, Portugal Institute Science Innovation Bio-Sustainability, 3 Estación de Ciencias Marinas Toralla (ECIMAT), Spain Ecology Animal Vigo, Problem statement: Assessment species composition...
Abstract Despite the growing use and potential of DNA metabarcoding to improve expedite macrozoobenthos monitoring, its employment in Water Framework Directive (WFD) monitoring transitional ecosystems still remains largely unexplored pending proof-of-concept studies. In current study, we addressed this research gap by building upon biomonitoring network program Portuguese Environmental Agency (APA) benchmark against morphology-based approach for characterizing macrozoobenthic communities. We...