A5102951434- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Acute Myeloid Leukemia Research
- Microfluidic and Bio-sensing Technologies
- Nanofabrication and Lithography Techniques
- Transition Metal Oxide Nanomaterials
- Cancer Research and Treatments
- Near-Field Optical Microscopy
- Peptidase Inhibition and Analysis
- Sarcoma Diagnosis and Treatment
- Radiopharmaceutical Chemistry and Applications
- RNA modifications and cancer
- TiO2 Photocatalysis and Solar Cells
- RNA Interference and Gene Delivery
- Quantum Dots Synthesis And Properties
- Polydiacetylene-based materials and applications
- DNA and Nucleic Acid Chemistry
- Building Energy and Comfort Optimization
- Microfluidic and Capillary Electrophoresis Applications
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Antimicrobial Peptides and Activities
Second Affiliated Hospital of Zhejiang University
2021-2024
Zhejiang University
2014
Digital multiplexed homogeneous immunoassay is supposed to have the advantages of high sensitivity, analytical throughput, small sampling errors, and low consumption. We present a spectral imaging-based multiplex, homogenous by counting sandwich-structured immunocomplexes in form quantum dot (QD) aggregates. As proof concept, method was utilized detect two tumor biomarkers: carcino-embryonic antigen (CEA) α-fetoprotein (AFP). The immunocomplex induced CEA contained QD 655 585 were recognized...
The development of multiplexed immunoassays is impeded by the difficulty in distinguishing labeled immunocomplexes from free probes and nonspecifically bound probes. Here, we attempted to overcome this issue counting core–satellite-structured simultaneously using dark-field fluorescence microscopy. tumor biomarkers carcinoembryonic antigen (CEA), α-fetoprotein (AFP), prostate-specific (PSA) were chosen as model targets. Gold nanoparticles (AuNPs) with diameters 70 nm coated detection...
Quantum dot (QD)-based digital immunoassays play an important role in ultrasensitive biomarker detection. However, the requirement of objective with a high numerical aperture (NA) limits application this immunoassay. Here, high-quality imaging massive single-QDs was achieved by combination air (20×/0.4 NA) and liquid-immersed microspheres (150 μm, n = 2.2). The signal-to-noise ratio comparable to that 100×/1.4 NA oil objective. Digital analysis prostate-specific antigen (PSA) performed...
Amplification-free digital immunoassays (DIAs) typically utilize optical nanoparticles to enhance single immunocomplex molecule detection. The efficiency and uniformity of transferring the from a bulk solution solid surface determine limit detection (LOD) accuracy DIAs. Previous methods suffer issues like low efficiency, nonuniform distribution, particle aggregation. Here, we present novel technique named synergistic sedimentation Brownian motion suppression dehydration transfer (SynSed) for...
Rapid, simple and sensitive screening of foodborne pathogens is great significance to ensure food safety. In this study, an aptamer-functionalized polydiacetylene (Apta-PDA) biosensor was developed for the detection E. coli O157:H7, S. typhimurium or V. parahaemolyticus. First, aptamers responding target bacteria were modified on surface magnetic beads by covalent binding form MBs-oligonucleotide conjugates bacterial enrichment. Then, Apta-PDA obtained connecting PDA nanovesicles using...
ABSTRACT By taking advantage of microflow injection chemiluminescence analysis, we developed a distinctive microfluidic bioassay method based on G‐Quadruplex DNAzyme‐enhanced for the determination K + in human serum. AGRO100, G‐rich oligonucleotide with high hemin binding affinity was primarily selected as recognition element. In presence , AGRO100 folded into G‐quadruplex and bound to form DNAzyme, which catalyzed oxidation luminol by H 2 O produce chemiluminescence. The intensity increased...
A contactless and ball-lens assisted sensitivity improvement method was present for the fluorescence or luminescence immunoassay in microchannel.
Abstract Myelodysplastic syndrome (MDS) is a neoplastic heterogeneous and pre-leukemic disease with poor clinical outcome due to the failure of current chemotherapeutic strategies target leukemic stem cells. Recently, we find that p21-activated kinase 5 (PAK5) overexpresses in MDS patients leukemia cell lines. The prognostic value PAK5 unclear although it has anti-apoptosis ability can promote survival mobility solid tumors. In this study, LMO2 co-expressed aberrant cells from MDS,...