- Plant nutrient uptake and metabolism
- Legume Nitrogen Fixing Symbiosis
- Microbial Metabolites in Food Biotechnology
Maria Curie-Skłodowska University
2023
The biosynthesis of subunits rhizobial exopolysaccharides is dependent on glycosyltransferases, which are usually encoded by large gene clusters. PssA a member family phosphoglycosyl transferases catalyzing the transfer phosphosugar moiety to polyprenol phosphate; thus, it can be considered as priming glycosyltransferase commencing synthesis EPS repeating units in Rhizobium leguminosarum. comprehensive analysis protein features performed this work confirmed its specificity for UDP-glucose...
The Pss-I region of Rhizobium leguminosarum bv. trifolii TA1 comprises more than 20 genes coding for glycosyltransferases, modifying enzymes, and polymerization/export proteins, altogether determining the biosynthesis symbiotically relevant exopolysaccharides. In this study, role homologous PssG PssI glycosyltransferases in exopolysaccharide subunit synthesis were analyzed. It was shown that glycosyltransferase-encoding part a single large transcriptional unit with potential downstream...