A5037148316- Molecular Biology Techniques and Applications
- Forensic and Genetic Research
- Environmental DNA in Biodiversity Studies
- Sperm and Testicular Function
- Gene expression and cancer classification
- Neuroscience and Neuropharmacology Research
- Cannabis and Cannabinoid Research
- Reproductive Biology and Fertility
- Metabolomics and Mass Spectrometry Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cell Image Analysis Techniques
- Ethics in Clinical Research
- Reproductive biology and impacts on aquatic species
- Identification and Quantification in Food
- Neuroscience of respiration and sleep
- Microfluidic and Capillary Electrophoresis Applications
- Forensic Fingerprint Detection Methods
- Microfluidic and Bio-sensing Technologies
Virginia Commonwealth University
2015-2025
Wake Forest University
2015
Queen Mary University of London
2015
Indiana University Bloomington
2015
Cannabinoid CB<sub>1</sub> receptors (CB<sub>1</sub>Rs) mediate the presynaptic effects of endocannabinoids in central nervous system (CNS) and most behavioral exogenous cannabinoids. receptor–interacting protein 1a (CRIP<sub>1a</sub>) binds to CB<sub>1</sub>R C-terminus can attenuate constitutive CB<sub>1</sub>R-mediated inhibition Ca<sup>2+</sup> channel activity. We now demonstrate cellular colocalization CRIP<sub>1a</sub> at neuronal elements CNS show that inhibits both...
In recent years, DNA analysis techniques have drastically increased in sensitivity, allowing for low template samples to be more easily detected and used identification. Since the problems inherent are exacerbated mixture samples, it would advantageous incorporate an assay earlier workflow that could detect a and, potentially, determine number of contributors. Some real-time PCR instruments high-resolution melt curve (HRM) capabilities, opportunity integrate HRM screening into commercial...
Sexual assault evidence often contains sperm cells, which are typically separated from nonsperm cells using manual differential lysis procedures. The goal of this study was to evaluate the automated QIAGEN QIAcube for purpose and compare it organic methods DNA yields STR profile data assessment. were determined by qPCR, followed multiplex amplification, CE analysis, mixture interpretation. method capable effective cell separation, producing sufficient amplification. Further, fraction...
This work describes the development of a novel microdevice for forensic DNA processing reference swabs. incorporates an enzyme‐based assay preparation, which allows faster times and reduced sample handling. Infrared‐mediated PCR (IR‐PCR) is used STR amplification using custom reaction mixture, allowing loci in 45 min while circumventing limitations traditional block thermocyclers. Uniquely positioned valves coupled with simple rotational platform are to exert fluidic control, eliminating...
Abstract Despite the improvements in forensic DNA quantification methods that allow for early detection of low template/challenged samples, complicating stochastic effects are not revealed until final stage analysis workflow. An assay would provide genotyping information at earlier examiners to make critical adjustments prior STR amplification allowing potentially exclusionary be immediately reported. Specifically, qPCR instruments often have dissociation curve and/or high-resolution melt...
Abstract DNA extractions of semen samples commonly utilize dithiothreitol (DTT) to reduce and disrupt disulfide bonds. Although traditional extraction techniques remove DTT before downstream analyses, the forensic community has recently explored Y‐screening, direct amplification, cell lysis assays that omit purification but employ reducing agents lyse spermatozoa. This study examined impact residual on processes involving fluorescent dyes. Quantification using Investigator ® Quantiplex HYres...
Due to recent improvements in forensic DNA testing kit sensitivity, there has been an increased demand the criminal justice community revisit past convictions or cold cases. Some of these cases have little biological evidence other than touch form archived latent fingerprint lift cards. In this study, a previously developed optimised workflow for sample type was tested on aged fingerprints determine if improved short tandem repeat (STR) profiles could be obtained. Two-year-old samples...
Abstract The prevalence of sexual assault cases and increasingly sensitive DNA analysis methods have resulted in kit backlogs the United States. Although traditional extraction purification utilizing detergents, proteinase K, DTT been primary technique for lysing sperm cell fractions from these samples, it is labor‐intensive inefficient regarding time recovery – hindering ability forensic analysts to keep pace with evidence submissions. Thus, this study examined seven alternative lysis...
Abstract Sample collection at the crime scene can introduce variations in DNA recovery based upon substrate from which a sample is collected, material of device used, or storage conditions after collection. There are many factors during this process that degrade drying and storage, before extraction be performed. The purpose study was to evaluate compare performance standard cotton swab with Bode BioSafe ® swab, includes both desiccant head proprietary compounds prevent degradation...
As DNA technology becomes increasingly sensitive, forensic laboratories are receiving more low-template samples. These samples, already low in content, become even challenging to process as the available further reduced during extraction step. In this study, two modifications were tested determine if cause of loss could be identified and mitigated. A double lysis technique was used test for sample collection substrate, lysate eluates re-extracted from inefficient binding silica column. Both...