A5035038810- Renal and related cancers
- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- Renal cell carcinoma treatment
- Prostate Cancer Treatment and Research
- COVID-19 Clinical Research Studies
- Cancer, Lipids, and Metabolism
- Chronic Lymphocytic Leukemia Research
- Estrogen and related hormone effects
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Renal Transplantation Outcomes and Treatments
- Vasculitis and related conditions
- Pediatric Urology and Nephrology Studies
- Hippo pathway signaling and YAP/TAZ
- SARS-CoV-2 and COVID-19 Research
- Molecular Biology Techniques and Applications
- Bone and Dental Protein Studies
- Metabolism, Diabetes, and Cancer
- Cervical Cancer and HPV Research
- Genital Health and Disease
- Lymphoma Diagnosis and Treatment
- Birth, Development, and Health
- Multiple Myeloma Research and Treatments
- Hormonal Regulation and Hypertension
Virginia Commonwealth University
2021-2024
Children's Hospital of Richmond at VCU
2022-2024
Virginia Commonwealth University Medical Center
2023
University of Washington
2020-2022
Combined Military Hospital
2021
Rawalpindi Medical University
2021
Hunter Holmes McGuire VA Medical Center
2021
Saint Louis University
2018
University of Houston
2011-2015
Estrogen receptor β (ERβ) is activated in the prostate by 5α-androstane-3β,17β-diol (3β-Adiol) where it exerts antiproliferative activity. The proliferative action of androgen 5α-dihydrotestosterone (DHT). Thus, growth governed balance between and ERβ activation. 3β-Adiol a high-affinity ligand agonist derived from DHT 3-keto reductase/3β-hydroxysteroid dehydrogenase enzymes. Here, we demonstrate that, when expressed living cells containing an estrogen response element-luciferase reporter,...
Significance Statement Whether kidney tubules exposed to nephrotic-range serum protein levels during glomerular filtration are injured remains controversial. This is important because acutely can trigger inflammation and fibrosis that lead irreversible injury CKD. Using human tubule 3D microphysiologic systems orthogonal epigenomic approaches, we found serum, but not its major component albumin, directly induces tubular secretion of proinflammatory components. Similar changes were observed...
Extracellular matrix (ECM)-induced β1-integrin-FAK signaling promotes cell attachment, survival, and migration of cancer cells in a distant organ so as to enable metastasis. However, mechanisms governing activation the remain incompletely understood. Here, we report that vasodilator-stimulated phosphoprotein (VASP), an actin binding protein, is required for ECM-mediated β1-integrin-FAK-YAP1/TAZ gastrointestinal (GI) their liver In patient-derived samples, VASP upregulated 53 63 colorectal...
Hereditary, hormonal, and behavioral factors contribute to the development of breast cancer. Alcohol consumption is a modifiable behavior that linked increased cancer risks associated with hormone-dependent cancers as well disease progression recurrence following endocrine treatment. In this study we examined molecular mechanisms action alcohol by applying molecular, genetic, genomic approaches in characterizing its effects on estrogen receptor (ER)-positive cells. Treatments promoted cell...
Differentiated vulvar intraepithelial neoplasia (dVIN) is a human papillomavirus-independent lesion with the potential for rapid progression to invasive squamous cell carcinoma (SCC). The histopathologic features of dVIN are diverse, have overlapping characteristics lichen sclerosus (LS) and simplex chronicus (LSC), may be diagnosed by dermatopathologists or gynecologic pathologists because vulva's anatomic location.To identify salient dVIN, particularly those that predict SCC, evaluate...
The coronavirus disease 2019 (COVID-19) has caused many different complications including immune-related conditions. Hereby, we report a case of possible association between COVID-19 infection and de novo anti-neutrophil cytoplasmic antibodies (ANCA)-associated vasculitis presenting with severe pulmonary-renal syndrome as rare complication infection. We had 53-year-old male patient who was admitted for pneumonia complicated by septic shock acute respiratory distress syndrome. He responded to...
The diagnosis and reporting of prostatic adenocarcinoma have evolved from the classic framework promulgated by Dr Donald Gleason in 1960s into a complex nuanced system grading that nonetheless retains essence his remarkable observations. criteria for “Gleason patterns” originally proposed been continually refined consensuses field, scores stratified patient-friendly set prognostically validated widely adopted Grade Groups. One product this successful approach has opportunity pathologists to...
Mild histologic lesions of tubulo-interstitial inflammation could represent a "response-to-wounding" rather than allorecognition. Tissue gene expression may complement histopathology for T-cell-mediated rejection (TCMR) diagnostics.
Abstract The renal medulla is a specialized region of the kidney with important homeostatic functions. It has also been implicated in genetic and developmental disorders ischemic drug-induced injuries. Despite its role function disease, medulla’s baseline gene expression epigenomic signatures have not well described adult human kidney. Here we generate analyze (RNA-seq), chromatin accessibility (ATAC-seq) conformation (Hi-C) data from cortex medulla. Using our carefully annotated specimens,...
Abstract Proteinuria, the spillage of serum proteins into urine, is a feature glomerulonephritides, podocyte disorders and diabetic nephropathy. However, response tubular epithelial cells to protein exposure has not been systematically characterized. Using transcriptomic profiling we studied serum-induced changes in primary human cultured 3D microphysiological devices. Serum induced cellular proliferation, cytokine secretion activated coordinated stress response. We orthogonally confirmed...
Abstract Introduction: Liver metastasis of pancreatic or colorectal tumors is a major cause cancer-related death worldwide. When cancer cells reach the liver parenchyma and make direct contact with surrounding extracellular matrix (ECM), β1-integrin-FAK signaling activated allowing attach, migration, proliferate. Thus, axis key for to colonize liver. However, mechanisms governing β1-integrin activation how this pathway promotes remain incompletely understood. Aim: We sought test hypothesis...
e15674 Background: Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer with 5 year survival of less than 7%. Vasodilator stimulated phosphoprotein (VASP) involved in cytoskeleton assembly and reorganization, which frequently affected malignant transformation. The role VASP the pathogenesis PDAC has not been previously reported. Here, we investigated human PDAC. Methods: Tissue samples from 53 patients (with or without PanIN benign duct), including 50 tissue arrays (20 mm2of each...
Athreya, Akshay; Gupta, Gaurav; Azhar, Ambreen; Kamal, Layla; Muthusamy, Selvaraj; Saeed, Muhammad I.; Khan, Aamir Author Information
Lidberg, Kevin; Muthusamy, Selvaraj; Adil, Mohamed; Reichel, Jonathan; Himmelfarb, Kelly, Edward J.; Akilesh, Shreeram Author Information
Journal of the American Society Nephrology 31(10S):p 38, October 2020. | DOI: 10.1681/ASN.20203110S138b
Abstract BACKGROUND AND AIMS The parenchyma of the kidney is divided into two major structures, outer cortex and inner medulla. medulla a key regulator salt water balance commonly affected by congenital anomalies urinary tract. Its cell types represent origin several monogenic diseases such as ADTKD-UMOD Bartter-SLC12A1. Currently, there lack human medulla-specific gene expression data from sufficiently large sets primary samples, which has hampered our ability to identify regulatory...
A 64-year-old African American male presented to the emergency department with subacute low back pain for two weeks and decreased urine output. He was found have a potassium level of 9.2 mmol/L uremic creatinine 28.5 mg/dL blood urea nitrogen (BUN) 201 mg/dL. also tested positive COVID-19. then started on continuous renal replacement therapy (CRRT). His urinalysis showed more than 500 protein. workup multiple myeloma conducted, protein electrophoresis test free lambda light chains 17,700...