A5082395135- Developmental Biology and Gene Regulation
- Cellular Mechanics and Interactions
- Pluripotent Stem Cells Research
- Microtubule and mitosis dynamics
- 3D Printing in Biomedical Research
- Neurobiology and Insect Physiology Research
- Planarian Biology and Electrostimulation
- Hedgehog Signaling Pathway Studies
- Cellular transport and secretion
- Neurogenesis and neuroplasticity mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Gene Regulatory Network Analysis
- Epigenetics and DNA Methylation
- Nasal Surgery and Airway Studies
- Protist diversity and phylogeny
- TGF-β signaling in diseases
- Hippo pathway signaling and YAP/TAZ
- Congenital heart defects research
- dental development and anomalies
- Wnt/β-catenin signaling in development and cancer
- Zebrafish Biomedical Research Applications
- Congenital Ear and Nasal Anomalies
- Meta-analysis and systematic reviews
- Nuclear Structure and Function
- Erythrocyte Function and Pathophysiology
Institute of Science and Technology Austria
2010-2024
The Francis Crick Institute
2010-2017
Medical Research Council
2013-2014
University of Geneva
2007-2011
Max Planck Institute of Molecular Cell Biology and Genetics
2006-2008
Max Planck Institute for the Physics of Complex Systems
2007
Lomonosov Moscow State University
2007
Max Planck Society
2006
In the developing fly wing, secreted morphogens such as Decapentaplegic (Dpp) and Wingless (Wg) form gradients of concentration providing positional information. Dpp forms a longer-range gradient than Wg. To understand how range is controlled, we measured four key kinetic parameters governing morphogen spreading: production rate, effective diffusion coefficient, degradation immobile fraction. The had different values for versus addition, Dynamin-dependent endocytosis was required spreading...
Sizing Signals Growth regulation of the Drosophila wing imaginal disc critically depends on Decapentaplegic (Dpp) morphogen gradient. How a graded Dpp signal is interpreted by cells to control homogeneous tissue growth remains unclear. Wartlick et al. (p. 1154 ; see Perspective Le Goff and Lecuit ) address this question measuring spatial temporal changes concentration signaling activity during phase quantifying cell proliferation parameters in discs. Both modeling experimental findings...
Like many developing tissues, the vertebrate neural tube is patterned by antiparallel morphogen gradients. To understand how these inputs are interpreted, we measured signaling and target gene expression in mouse embryos chick ex vivo assays. From data, derived validated a characteristic decoding map that relates input to positional identity of progenitors. Analysis observed responses indicates underlying interpretation strategy minimizes patterning errors response joint noisy opposing We...
Morphogens are secreted signalling molecules that act in a graded manner to control the pattern of cellular differentiation developing tissues. An example is Sonic hedgehog (Shh), which acts several vertebrate tissues, including central nervous system, provide positional information during embryonic patterning. Here we address how Shh assigns identities distinct neuronal subtype progenitors throughout ventral neural tube. Assays intracellular signal transduction and gene expression indicate...
Development requires tissue growth as well cell diversification. To address how these processes are coordinated, we analyzed the development of molecularly distinct domains neural progenitors in mouse and chick tube. We show that during development, undergo changes size do not scale with overall size. Our data domain proportions first established by opposing morphogen gradients subsequently controlled domain-specific regulation differentiation rate but differences proliferation rate....
In the vertebrate neural tube, morphogen Sonic Hedgehog (Shh) establishes a characteristic pattern of gene expression. Here we quantify Shh gradient in developing mouse tube and show that while amplitude increases over time, activity pathway transcriptional effectors, Gli proteins, initially but later decreases. Computational analysis suggests three mechanisms could contribute to this adaptation: upregulation inhibitory receptor Ptch1, downregulation differential stability active inactive...
Morphogen concentration gradients provide positional information by activating target genes in a concentration-dependent manner. Recent reports show that the gradient of syncytial morphogen Bicoid seems to precise determine gene domains. For secreted morphogenetic ligands, precision gradients, signal transduction and reliability expression domains have not been studied. Here we investigate these issues for TGF-beta-type Dpp. We first studied theoretically how cell-to-cell variability source,...
As developing tissues grow in size and undergo morphogenetic changes, their material properties may be altered. Such changes result from tension dynamics at cell contacts or cellular jamming. Yet, many cases, the mechanisms controlling physical state of growing are unclear. We found that early developmental stages, epithelium mouse spinal cord maintains both high junctional fluidity. This is achieved via a mechanism which interkinetic nuclear movements generate area drive extensive...
During neural tube (NT) development, the notochord induces an organizer, floorplate, which secretes Sonic Hedgehog (SHH) to pattern progenitors. Conversely, NT organoids (NTOs) from embryonic stem cells (ESCs) spontaneously form floorplates without notochord, demonstrating that can self-organize inducers. Here, we investigated floorplate self-organization in clonal mouse NTOs. Expression of marker FOXA2 was initially spatially scattered before resolving into multiple clusters, underwent...
We study the mechanics of tissue growth via cell division and death (apoptosis). The rearrangements cells can on large scales times be captured by a continuum theory which describes as an effective viscous material with active stresses generated division. effects anisotropies show that average cellular trajectories exhibit anisotropic scaling behaviors. If apoptosis balance, there is no net growth, but for undergoes spontaneous shear deformations. Our description relevant developing tissues...
Facial shape is the basis for facial recognition and categorization. features reflect underlying geometry of skeletal structures. Here, we reveal that cartilaginous nasal capsule (corresponding to upper jaw face) shaped by signals generated neural structures: brain olfactory epithelium. Brain-derived Sonic Hedgehog (SHH) enables induction septum posterior capsule, whereas formation a roof controlled from Unexpectedly, cartilage turned out be important shaping membranous bones during...
ABSTRACT Cell division, movement and differentiation contribute to pattern formation in developing tissues. This is the case vertebrate neural tube, which neurons differentiate a characteristic from highly dynamic proliferating pseudostratified epithelium. To investigate how progenitor proliferation affect cell arrangement growth of we used experimental measurements develop mechanical model apical surface neuroepithelium that incorporates effect interkinetic nuclear spatially varying rates...
Abstract As organs are remodelled by morphogenetic changes and pattern formation during development, their material properties may change. To address whether how this occurs in the mouse neural tube, we combined highly resolved mosaic analysis, biophysical modelling perturbation experiments. We found that at early developmental stages neuroepithelium surprisingly maintains both high junctional tension fluidity. This is achieved via a previously unrecognized mechanism which interkinetic...
The development of a functional tissue requires coordination the amplification progenitors and their differentiation into specific cell types. molecular basis for this during myotome ontogeny is not well understood. Dermomytome that colonize first acquire myocyte identity subsequently proliferate as Pax7-expressing before undergoing terminal differentiation. We show dynamics sonic hedgehog (Shh) signaling crucial transition in both avian mouse embryos. Initially, Shh ligand emanating from...