A5014693062- Nitric Oxide and Endothelin Effects
- Traumatic Brain Injury and Neurovascular Disturbances
- Sulfur Compounds in Biology
- Neuropeptides and Animal Physiology
- Neuroscience of respiration and sleep
- Eicosanoids and Hypertension Pharmacology
- Wound Healing and Treatments
- Pharmacogenetics and Drug Metabolism
- Receptor Mechanisms and Signaling
- Immune Response and Inflammation
- Hemoglobin structure and function
- Obstructive Sleep Apnea Research
- Exercise and Physiological Responses
- Stress Responses and Cortisol
- Analytical Chemistry and Sensors
- Respiratory Support and Mechanisms
- Gas Sensing Nanomaterials and Sensors
- Advanced Drug Delivery Systems
- Heme Oxygenase-1 and Carbon Monoxide
- Advanced Chemical Sensor Technologies
- Adipose Tissue and Metabolism
- Pancreatitis Pathology and Treatment
- Cholinesterase and Neurodegenerative Diseases
- Surgical Sutures and Adhesives
- High Altitude and Hypoxia
National University Health System
2024
National University of Singapore
2006-2023
University College London
2014-2023
Temasek Polytechnic
2017-2020
National University Hospital
1996-2020
The Royal Free Hospital
2014-2019
DSO National Laboratories
2007-2018
Nanyang Technological University
2007-2017
Clinical Nutrition Research Centre
2013-2015
Environmental Research Institute
2014
Hydrogen sulfide (H2S) is a naturally occurring gas with potent vasodilator activity. Cystathionine-γ-lyase (CSE) and cystathionine-β-synthase (CBS) utilize l-cysteine as substrate to form H2S. Of these two enzymes, cystathionine-γ-lyase believed be the key enzyme that forms H2S in cardiovascular system. Whilst has been reported relax precontracted rat arteries vitro lower blood pressure rat, its effect an inflammatory condition such acute pancreatitis not previously reported. In this paper,...
Haemorrhagic shock (60 min) in the anaesthetized rat resulted a prolonged fall mean arterial blood pressure (MAP) and heart rate (HR). Pre-treatment (30 min before shock) or post-treatment after with inhibitors of cystathionine gamma lyase (CSE; converts cysteine into hydrogen sulphide (H(2)S)), dl-propargylglycine beta-cyanoalanine (50 mg kg(-1), i.v.), glibenclamide (40 i.p.), produced rapid, partial restoration MAP HR. Neither saline nor DMSO affected Plasma H(2)S concentration was...
Recent studies have implied that hydrogen sulfide (H 2 S) plays a crucial role in several inflammatory conditions. However, so far little is known about the mechanism by which H S provokes response sepsis. Thus aim of this study was to investigate if regulates sepsis-associated systemic inflammation and production proinflammatory mediators via activation NF-κB. Male Swiss mice were subjected cecal ligation puncture (CLP)-induced sepsis treated with dl-propargylglycine (PAG; 50 mg/kg ip),...
During sepsis, activation of phagocytes leads to the overproduction proinflammatory cytokines, causing systemic inflammation. Despite substantial information regarding underlying molecular mechanisms that lead several elements in pathway remain be elucidated. We found enzyme sphingosine kinase 1 (SphK1) is up-regulated stimulated human and peritoneal patients with severe sepsis. Blockade SphK1 inhibited phagocyte production endotoxin-induced cytokines. observed protection against sepsis mice...
Lipopolysaccharide (LPS) is a toxic inflammatory stimulator released from the outer cell membrane of Gram-negative bacteria, known to be directly related to, for example, septic shock, that causes millions casualties annually. This number could potentially lowered significantly if specific, sensitive, and more simply applicable LPS biosensors existed. In this work, we present facile, sensitive selective sensor, developed by assembling tetramethylrhodamine-labeled LPS-binding peptides on...
Burn infliction techniques are poorly described in rat models. An accurate study can only be achieved with wounds that uniform size and depth. We describe a simple reproducible method for creating consistent burn rats.Ten male Sprague-Dawley rats were anesthetized dorsum shaved. A 100 g cylindrical stainless-steel rod (1 cm diameter) was heated to 100℃ boiling water. Temperature monitored using thermocouple. performed two consecutive toe-pinch tests on different limbs assess the depth of...
The prevalence and incidence of kidney stone disease have increased markedly during the past several decades, studies demonstrated that inappropriate dietary habits are leading to more obesity overweight (OW) in children adults, which may be important formation. Obese OW patients share most same risk factors for cardiovascular morbidity, while impact being OW, rather than obese, on urinary metabolic parameters formers (KSF) is less well known. aims this study were investigate parameters,...
Hydrogen sulphide (H(2)S) is a naturally occurring gas, with potent vasodilator activity. In this report, we identify role for H(2)S in carrageenan-induced hindpaw oedema the rat. Intraplantar injection of carrageenan (150 microl, 2% (w v(-1))) resulted an increase synthesising enzyme activity and increased myeloperoxidase (MPO) Pretreatment (i.p. 60 min before carrageenan) DL-propargylglycine (PAG, 25-75 mg kg(-1)), inhibitor cystathionine-gamma-lyase (CSE), significantly reduced...
Abstract Polymeric micelles self‐assembled from cholesterol‐conjugated poly(ethylene glycol) (PEG) and anchored with transcriptional activator TAT peptide (TAT‐PEG‐b‐Col) were fabricated for delivery of antibiotics across the blood–brain barrier (BBB). Ciprofloxacin, which demonstrated a high bactericidal effect, was efficiently loaded into by membrane dialysis method. The ciprofloxacin‐loaded characterized via dynamic light scattering SEM. spherical in nature, having an average diameter...
Abstract Hydrogen sulfide (H2S) is recognized increasingly as a proinflammatory mediator in various inflammatory conditions. Here, we have investigated the role of H2S regulating expression some endothelial adhesion molecules and recruitment leukocytes to inflamed sites sepsis. Male Swiss mice were subjected cecal ligation puncture (CLP)-induced sepsis treated with saline (i.p.), DL-propargylglycine (PAG; 50 mg/kg, i.p.), an inhibitor formation or NaHS (10 donor. PAG was administered 1 h...
Abstract Hydrogen sulfide (H2S) up-regulates inflammatory response in several diseases. However, to date, little is known about the molecular mechanism by which H2S provokes sepsis. Thus, aim of this study was investigate signaling pathway underlying proinflammatory role cecal ligation and puncture (CLP)-induced Male Swiss mice were subjected CLP treated with dl-propargylglycine (PAG; 50 mg/kg i.p., an inhibitor formation), NaHS (10 mg/kg, donor), or saline. PAG administered 1 h before CLP,...