A5040848292- Analytical chemistry methods development
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Sensors
- Alzheimer's disease research and treatments
- Proteins in Food Systems
- Advanced biosensing and bioanalysis techniques
- Polymer Surface Interaction Studies
- Amyloidosis: Diagnosis, Treatment, Outcomes
University of Potsdam
2013-2019
Fraunhofer Institute for Cell Therapy and Immunology
2015
Fraunhofer Institute for Biomedical Engineering
2012
Abstract A novel strategy to prepare a selective ultrathin molecularly imprinted polymer (MIP) film directly on the gold‐based transducer surface for peptide and protein detection in aqueous solution is demonstrated using combination of epitope‐ electrochemical imprinting approach. The synthetic derived from surface‐exposed C‐terminus cytochrome c (Cyt c, residues 96–104) selected as template imprinting. It labeled with fluorescent dye order quantitatively evaluate all stages process terms...
Effective recognition of enzymatically active tetrameric acetylcholinesterase (AChE) is accomplished by a hybrid nanofilm composed propidium‐terminated self‐assembled monolayer (Prop‐SAM) which binds AChE via its peripheral anionic site (PAS) and an ultrathin electrosynthesized molecularly imprinted polymer (MIP) cover layer novel carboxylate‐modified derivative 3,4‐propylenedioxythiophene. The rebinding the to MIP/Prop‐SAM covered electrode detected measuring in situ enzymatic activity....
For the first time a molecularly imprinted polymer (MIP) with direct electron transfer (DET) and bioelectrocatalytic activity of target protein is presented. Thin films MIPs for recognition hexameric tyrosine-coordinated heme (HTHP) have been prepared by electropolymerization scopoletin after oriented assembly HTHP on self-assembled monolayer (SAM) mercaptoundecanoic acid (MUA) gold electrodes. Cavities which should resemble shape size were formed template removal. Rebinding sums up...
Abstract Hybrid architectures which combine a MIP with an immobilized “affinity ligand” or biocatalyst sum up the advantages of both components. In this paper, hybrid combining layer molecularly imprinted electropolymer mini‐enzyme self‐assembled monolayer will be presented. (i) Microperoxidase‐11 (MP‐11) catalyzed oxidation drug aminopyrine on product‐imprinted sublayer: The peroxide dependent conversion analyte takes place in MP‐11 containing top indicator electrode. hierarchical...
Molecularly imprinted polymers (MIPs) have the potential to complement antibodies in bioanalysis, are more stable under harsh conditions, and potentially cheaper produce. However, affinity especially selectivity of MIPs general lower than those their biological pendants. Enzymes useful tools for preparation both low high-molecular weight targets: As a green alternative well-established methods chemical polymerization, enzyme-initiated polymerization has been introduced removal protein...
Abstract A new approach for synthesizing a “vectorially” imprinted polymer (VIP) is presented the microbial cytochrome P450cam enzyme. surface attached binding motif of natural reaction partner target protein, putidaredoxin (Pdx), anchor to underlying transducer. The 15 amino acid peptide anchor, which stems from largest continuous chain within site Pdx was modified: (i) internal cysteines were replaced by serines prevent disulfide bond formation; (ii) 2 ethylene glycol units N‐terminus as...
On page 5178, R. E. Gyurcsányi, F. W. Scheller, and co-workers demonstrate specific recognition of acetylcholinesterase, a potential marker Alzheimer's disease, by hybrid material which shows threefold selectivity gain the “shape-specific” electrosynthesized molecularly imprinted polymer, oriented binding to propidium layer signal generation exclusively enzyme bound nanofilm. Acetylcholinesterase is detected in nanomolar range, whilst interfering proteins are effectively suppressed.
The talk describes the electrosynthesis and analytical performances of MIPs for following proteins: Acetylcholine esterase, Cytochrome P450 BM3, P450cam, Laccase, Tyrosinase, c, Ferritin, Transferrin, Concanavalin A Human Serum Albumin.These are synthesized with only ONE monomer (and even without a cross-linker) exhibit measuring ranges in micromolar up to sub-nanomolar concentration range.