A5022754182- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Lipid Membrane Structure and Behavior
- Phagocytosis and Immune Regulation
- Animal Virus Infections Studies
- Nanoparticle-Based Drug Delivery
- CAR-T cell therapy research
- Analytical Chemistry and Sensors
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Conducting polymers and applications
- CRISPR and Genetic Engineering
- Chemical synthesis and pharmacological studies
- Molecular Sensors and Ion Detection
- Biomedical and Engineering Education
- DNA and Nucleic Acid Chemistry
- Biomedical Ethics and Regulation
- Gold and Silver Nanoparticles Synthesis and Applications
AstraZeneca (United Kingdom)
2018-2024
University of Nottingham
2014-2015
mRNA lipid nanoparticles (LNPs) are at the forefront of nucleic acid intracellular delivery, as exemplified by recent emergency approval two LNP-based COVID-19 vaccines. The success an LNP product largely depends on systematic optimisation four lipidic components, namely ionisable lipid, PEG structural and helper lipids. However, in vitro screening novel components compositions is limited low-throughput preparation. To address these issues, we herein present automated high-throughput...
Abstract Recently, lipid nanoparticles (LNPs) have attracted attention due to their emergent use for COVID‐19 mRNA vaccines. The success of LNPs can be attributed ionizable lipids, which enable functional intracellular delivery. Previously, the authors established an automated high‐throughput platform screen lipids and identified that generated using this technique show comparable or increased delivery in vitro as compared prepared traditional microfluidics techniques. In study, choose one...
Abstract Poor understanding of intracellular delivery and targeting hinders development nucleic acid‐based therapeutics transported by nanoparticles. Utilizing a siRNA‐targeting small molecule profiling approach with advanced imaging machine learning biological insights is generated into the mechanism lipid nanoparticle (MC3‐LNP) mRNA. This workflow termed Advanced Cellular Endocytic for Intracellular Delivery (ACE‐ID). A cell‐based assay perturbation 178 targets relevant to trafficking used...
Despite recent advances in the field of mRNA therapy, lack safe and efficacious delivery vehicles with pharmaceutically developable properties remains a major limitation. Here, we describe systematic optimisation lipid-peptide nanocomplexes for two murine cancer cell types, B16-F10 melanoma CT26 colon carcinoma as well NCI-H358 human lung bronchoalveolar cells. Different combinations lipids peptides were screened from an original nanocomplex formulation improved luciferase transfection vitro...
A special symposium of the Academy Pharmaceutical Sciences Nanomedicines Focus Group reviewed current status use nanomedicines for delivery biologics drugs. This meeting was particularly timely with recent approval first siRNA-containing product Onpattro™ (patisiran), which is formulated as a lipid nanoparticle intravenous infusion, and increasing interest in oral biologics. The challenges delivering such molecules were discussed specific emphasis on both across into cells. latest...
Measurement of intracellular acidification is important for understanding fundamental biological pathways as well developing effective therapeutic strategies. Fluorescent pH nanosensors are an enabling technology real-time monitoring acidification. The physicochemical characteristics can be engineered to target specific cellular compartments and respond external stimuli. Therefore, represent a versatile approach probing inside cells. components comprise pH-sensitive fluorophore (signal...
Modified messenger RNAs (mRNAs) hold great potential as therapeutics by using the body's own processes for protein production. However, a key challenge is efficient delivery of therapeutic mRNA to cell cytosol and productive translation. Lipid nanoparticles (LNPs) are most clinically advanced system nucleic acid delivery; however, relatively narrow index makes them unsuitable many applications. A obstacle development more potent LNPs limited mechanistic understanding interaction with cells....
Materials for delivery of oligonucleotides need to be simple produce and formulate yet effective<italic>in vivo</italic>to considered clinical applications.
Abstract Lipid nanoparticles (LNPs) have been demonstrated to hold great promise for the clinical advancement of RNA therapeutics. Continued exploration LNPs application in new disease areas requires identification and optimization leads a high throughput way. Currently available vivo screening platforms are well suited screen cellular uptake but less so functional cargo delivery. We report on platform which measures delivery using unique peptide ‘barcodes’. describe design selection...
With the launch of UK Academy Pharmaceutical Sciences Advanced Therapy Medicinal Products Focus Group in late 2020, a webinar series reviewing current and emerging trends cell gene therapy was held virtually May 2021. This timely given recent withdrawal United Kingdom from European Union global COVID-19 pandemic impacting all sectors pharmaceutical sciences research landscape globally UK. Delegates academic, industry, regulatory NHS attended session where challenges opportunities development...