A5020185077- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Cellular Mechanics and Interactions
- Single-cell and spatial transcriptomics
- Animal Behavior and Reproduction
- Retinal Development and Disorders
- Avian ecology and behavior
- Wildlife Ecology and Conservation
- Plant and animal studies
- Microtubule and mitosis dynamics
- Photoreceptor and optogenetics research
- Insect Pheromone Research and Control
- Molecular Biology Techniques and Applications
- Axon Guidance and Neuronal Signaling
- Click Chemistry and Applications
- bioluminescence and chemiluminescence research
- Genetics, Aging, and Longevity in Model Organisms
- Optical Coherence Tomography Applications
- Aquatic Invertebrate Ecology and Behavior
- Cephalopods and Marine Biology
- Caribbean and African Literature and Culture
- Migration, Health, Geopolitics, Historical Geography
- Advanced Electron Microscopy Techniques and Applications
Janelia Research Campus
2015-2024
Howard Hughes Medical Institute
2014-2024
Helix (United States)
2014-2016
National Institute of Mental Health
2006
National Institutes of Health
2006
University of California, Berkeley
1997-2000
University of Arizona
1997
The University of Texas at Austin
1991-1993
Indiana University Bloomington
1991
Connecticut College
1860
Abstract Understanding how the brain works in tight concert with rest of central nervous system (CNS) hinges upon knowledge coordinated activity patterns across whole CNS. We present a method for measuring an entire, non-transparent CNS high spatiotemporal resolution. combine light-sheet microscope capable simultaneous multi-view imaging at volumetric speeds 25-fold faster than state-of-the-art, whole-CNS assay isolated Drosophila larval and computational framework analysing multi-view,...
Coupling of autonomous cellular oscillators is an essential aspect circadian clock function but little known about its circuit requirements. Functional ablation the pigment-dispersing factor-expressing lateral ventral subset (LN V ) Drosophila neurons abolishes rhythms locomotor activity. The hypothesis that LN s synchronize oscillations in downstream was tested by rendering hyperexcitable via transgenic expression a low activation threshold voltage-gated sodium channel. When are made...
A subset of Drosophila neurons that expresses crustacean cardioactive peptide (CCAP) has been shown previously to make the hormone bursicon, which is required for cuticle tanning and wing expansion after eclosion. Here we present evidence CCAP-expressing (NCCAP) consist two functionally distinct groups, one releases bursicon into hemolymph other regulates its release. The first group, call NCCAP-c929, includes 14 bursicon-expressing abdominal ganglion lie within expression pattern...
Abstract Genetically encoded fluorescent calcium indicators allow cellular-resolution recording of physiology. However, bright, genetically targetable that can be multiplexed with existing tools in vivo are needed for simultaneous imaging multiple signals. Here we describe WHaloCaMP, a modular chemigenetic indicator built from bright dye-ligands and protein sensor domains. Fluorescence change WHaloCaMP results reversible quenching the bound dye via strategically placed tryptophan. is...
We present a method to automatically identify and track nuclei in time-lapse microscopy recordings of entire developing embryos. The combines deep learning global optimization. On mouse dataset, it reconstructs 75.8% cell lineages spanning 1 h, as compared 31.8% for the competing method. Our approach improves understanding where when fate decisions are made embryos, tissues, organs.
Abstract Genetically encoded fluorescent calcium indicators have revolutionized neuroscience and other biological fields by allowing cellular-resolution recording of physiology during behavior. However, we currently lack bright, genetically targetable in the near infrared that can be used animals. Here, describe WHaloCaMP, a modular chemigenetic indicator built from bright dye-ligands protein sensor domains targeted to specific cell populations. Fluorescence change WHaloCaMP results...
Axonal branching allows a neuron to connect several targets, increasing neuronal circuit complexity. While axonal is well described, the mechanisms that control it remain largely unknown. We find in Drosophila CNS branches develop through process of excessive growth followed by pruning. In vivo high-resolution live imaging developing brains as loss and gain function experiments show activation Epidermal Growth Factor Receptor (EGFR) necessary for branch dynamics final pattern. Live also...
Recent studies that have shown variation in avian foraging behavior can produce changes reproductive success and survival raised the question of role energy budgets determining other life-history traits birds. The study reported here describes how daily breeding populations zebra finch Taeniopygia guttata influenced rate at which broods were produced, size could be success-fully supported by their parents, subsequent parents. This was examined experimentally altering net gain four randomly...
ABSTRACT Behavioral and physiological evidence indicates that insect pheromone sensory neurons are able to resolve pulses of concentration as they occur downwind from a point source, but the abilities sensitive general odors respond pulsatile stimuli unknown. The temporal response characteristics olfactory female American cockroaches Periplaneta americana in were measured using series short odor (20–400 ms). Odor delivered sensilla moving airstream controlled by electromagnetic valves....
Abstract Glucose is arguably the most important molecule in metabolism, and its mismanagement underlies diseases of vast societal import, notably diabetes. Although glucose-related metabolism has been subject intense study for over a century, tools to track glucose living organisms with high spatio-temporal resolution are lacking. We describe engineering family genetically encoded sensors signal-to-noise ratio, fast kinetics affinities varying four orders magnitude (1 µM 10 mM). The allow...