A5062775495- Nanoparticle-Based Drug Delivery
- Hydrogels: synthesis, properties, applications
- Medical Imaging and Analysis
- RNA Interference and Gene Delivery
- Radiomics and Machine Learning in Medical Imaging
- Analytical chemistry methods development
- Advanced X-ray and CT Imaging
- Supramolecular Self-Assembly in Materials
- earthquake and tectonic studies
- Geological and Geochemical Analysis
- Lipid Membrane Structure and Behavior
- Analytical Chemistry and Sensors
- Protease and Inhibitor Mechanisms
- Medical Imaging Techniques and Applications
- Protein purification and stability
- Nanoplatforms for cancer theranostics
- Surgical Simulation and Training
- Micro and Nano Robotics
- Advanced Polymer Synthesis and Characterization
- High-pressure geophysics and materials
- Enzyme function and inhibition
- Drug Transport and Resistance Mechanisms
- Venomous Animal Envenomation and Studies
- Seismic Imaging and Inversion Techniques
- Polymer Surface Interaction Studies
Osaka University
2003-2024
University of California, Irvine
2020-2021
Kyushu University
2006-2020
Graduate School USA
2020
Detection of shallow slow earthquakes offers insight into the near-trench part subduction interface, an important region in development great earthquake ruptures and tsunami generation. Ocean-bottom monitoring offshore seismicity off southern Kyushu, Japan, recorded a complete episode low-frequency tremor, lasting for 1 month, that was associated with very-low-frequency (VLFE) activity plate interface. The tremor exhibited two migration modes reminiscent deep down-dip seismogenic zone some...
Although a number of procedures to create synthetic polymer nanoparticles (NPs) with an intrinsic affinity target biomacromolecules have been published, little has reported on strategies control the binding kinetics recognition. Here, we report enzyme-mimic strategy binding/dissociation rate constants NPs, which bind proteins through multipoint interactions, by taking advantage temperature-responsive coil-globule phase transition poly-N-isopropylacrylamide (PNIPAm)-based NPs. PNIPAm NPs...
Designed polymer hydrogel nanoparticles (NPs) capable of facilitating resolubilization and refolding an aggregated protein, positively charged lysozyme, are prepared. NPs designed to interact strongly with denatured lysozyme relatively weakly native facilitated lysozyme. Such could be prepared by copolymerizing optimized combinations populations functional monomers. The refolded showed conformation enzymatic activity. Eleven grams protein was 1 g NPs. However, having low affinity high both...
We describe a process for engineering synthetic polymer nanoparticle (NP) that functions as an effective, broad-spectrum metalloproteinase inhibitor. Inhibition is achieved by incorporating three functional elements in the NP: group interacts with catalytic zinc ion, functionality enhances affinity to substrate-binding pocket, and fine-tuning of chemical composition strengthen NP enzyme surface. The approach validated synthesis sequesters inhibits proteolytic activity snake venom...
Stimuli-responsive nanomaterials with multivalent ligands have attracted significant attention in the context of cancer chemotherapy and imaging. However, challenges remain such as non-selective stimuli response and/or existence triggers healthy regions addition to intrinsic heterogeneity that causes insufficient target recognition. In-spired by expression precise diverse functions biological machineries triggered specific protein-protein complexation conformational change, we report an...
Using GPS observations, we have detected an aseismic slip event on the intraplate Dedana Fault that was triggered by Iwate‐Miyagi Nairiku earthquake (Mw6.8, 13 Jun 2008 UTC) a nearby but separate fault. The observations cannot be satisfactorily explained in terms of either viscoelastic relaxation or poroelastic rebound, and explain observed displacement time series without presuming occurred Fault. This likely mainshock stress change. detection future such events faults is to require denser...
We report the effect of physical properties, such as flexibility and polymer density, nanogel particles (NPs) on association/dissociation rates constant (kon koff) equilibrium constants (Kd) multipoint protein recognition process. NPs having different flexibilities densities at 25 °C were synthesized by tuning cross-linking degrees volume phase transition (VPT) temperature. Rate quantified analyzing time course binding process monitored a quartz crystal microbalance (QCM). Both kon koff...
pKa tuning of Brønsted acids in synthetic nano-materials is great importance for the design ion exchange and bio-/molecular-separation media polymer catalysis. It has been reported that hydrogel nanoparticles with carboxylic show large reversible shifts response to thermal stimuli can be prepared by copolymerization N-isopropylacrylamide (NIPAm), acrylic (AAc) N,N'-methylene bisacrylamide (BIS) via proton imprinting polymerization process. However, range limited 5.3 7.5. In this study, we...
Abstract A metabolic cycle‐inspired hydrogel which exhibits the biomacromolecule‐fueled transient volume phase transition is reported. This has affinity and digestive capacity for a fuel α‐poly‐L‐lysine by incorporating acrylic acid trypsin. The captured transiently shrank owing to construction of electrostatic cross‐linkages. process was inherently connected with digestion these cross‐linkages release oligo‐lysine as waste, induced reswelling at equilibrium. change realized fuel‐stimulated...
The affinity of a synthetic polymer nanoparticle (NP) to target biomacromolecule is determined by the association and dissociation rate constants (kon, koff) interaction. individual rates their sensitivity local environmental influences are important factors for on-demand capture release biomacromolecule. Positively charged NPs small interfering RNA (siRNA) delivery case in point. knockdown efficacy siRNA can be strongly influenced binding kinetics NP. Here, we show that kon koff...
The acid dissociation constants (p Ka values) of Brønsted acids at the active sites proteins are reversibly modulated by intramolecular electrostatic interactions with neighboring ions in a reaction cycle. resulting p shift is crucial for to capture, transfer, and release target ions. On other hand, reversible modulation through synthetic polymer materials has seldom been realized because strongly shielded solvation water molecules aqueous media. Here, we prepared hydrogel nanoparticles...
This paper reviews methods for computer assisted medical intervention using statistical models and machine learning technologies, which would be particularly useful representing prior information of anatomical shape, motion, deformation to extrapolate intraoperative sparse data as well surgeons' expertise pathology optimize interventions. Firstly, we present a review recovery static structures by only without any preoperative patient-specific information. Then, motion are reviewed combining...
Hydrogel films that reversibly absorb salt in response to thermal stimuli are prepared and evaluated. Co-immobilization of temperature responsive poly(N-isopropylacrylamide) (polyNIPAm) nanoparticles (NPs) which carboxylic acids amines imprinted is crucial the design film. At high temperatures, bases NPs deionized form thus not capable capturing counterions. However, at low swell denature from structures absorbing salts by providing The absorbed released upon heating owing regeneration...
A biomimetic of endogenous tissue inhibitors metalloproteinases (TIMPs) was engineered by introducing three binding elements to a synthetic tetrapolymer. We evaluated the contribution composition, size, and shape TIMP-mimicking polymers inhibition BaP1, P-I class snake venom metalloproteinase (SVMP). Inhibition achieved when size linear polymer (LP) comparable or greater than that enzyme, indicating efficacy requires significant portion enzyme surface in vicinity active site. The low...
Most hydrogels have poor mechanical properties, severely limiting their potential applications, and numerous approaches been introduced to fabricate more robust durable examples. However, these systems consist of nonbiodegradable polymers which limit application in tissue engineering. Herein, we focus on the fabrication investigate influence hydrophobic segments ionic cross-linking properties for construction a tough, biodegradable hydrogel. A biodegradable, poly(γ-glutamic acid) polymer...
To evaluate the effects of transrectal compression prostate for intra-operative prostatic swelling and intraprostatic point shift during high-intensity focused ultrasound treatment localized cancer.Patients treated with whole-gland as primary monotherapy cancer were enrolled in study. Using standard method, volumes degassed water balloon covering probe 50 mL 80-160 mL, respectively. identify volume occupied by non-enhanced area, three-dimensional models reconstructed using contrast-enhanced...
In cancer therapy, a drug delivery system (DDS) has been widely studied to achieve selective accumulation at the tumor site. However, DDS still major drawback in that it requires multistep processes for intracellular delivery, resulting low efficiency of delivery. To overcome this problem, we recently reported molecular block (MB) disrupts cell membranes microenvironment using deoxycholic acid (DCA). MB showed considerable cytotoxicity even neutral pH, possibly due structural hydrophobic...
Since conventional molecular targeted drugs often result in side effects, the development of novel with both high efficacy and selectivity is desired. Simultaneous inhibition metabolically spatiotemporally related proteins/enzymes a promising strategy for improving therapeutic interventions cancer treatment. Herein, we report poly-α-l-glutamate-based polymer inhibitor that simultaneously targets proximal transmembrane enzymes under hypoxia, namely, carbonic anhydrase IX (CAIX) zinc-dependent...
Abiotic oligomeric ligands with a strong affinity for target peptide sequence were isolated by purification from pool of 30-mer acrylic random ter-oligomers that synthesized via controlled radical polymerization process. Our results indicate the suitable and/or stereochemical configurations can be as-polymerized co-polymers based on to target. This process will powerful tool development stable and inexpensive used detect, neutralize purify proteins epitope sequence.
We report a highly stretchable hydrogel based on the crosslinking structure between calcium cations and alendronates (ALN) conjugated with poly-γ-glutamate (γ-PGA), typical biodegradable polymer. γ-PGA ALN (γ-PGA-ALN) forms in aqueous solution containing CaCl2. The shows 2000% of stretchability reversible stretching without failure at strain 250%. fracture stress are tunable by varying concentration either γ-PGA-ALN or CaCl2, indicating importance fine-tuning density cross-linkage to control...
A metabolic cycle-inspired hydrogel which exhibits the biomacromolecule-fueled transient volume phase transition is reported. This has affinity and digestive capacity for a fuel α-poly-L-lysine by incorporating acrylic acid trypsin. The captured transiently shrank owing to construction of electrostatic cross-linkages. process was inherently connected with digestion these cross-linkages release oligo-lysine as waste, induced reswelling at equilibrium. change realized fuel-stimulated payload....
Improving the efficiency and selectivity of drug delivery systems (DDS) is still a major challenge in cancer therapy. Recently, low transport anticancer drugs using nanocarrier due to elimination carriers from blood circulation blocking by tumor stromal tissues surrounding cells has been reported. Furthermore, multiple steps are required for their intracellular delivery. We recently reported microenvironment-targeting therapy termed molecular block (MB) which induced cell death pH-driven...
A fundamental understanding of the design polymer ligands proximal enzymes is essential for precise targeting cancer cells, but it still in its infancy. In this study, we systematically investigated contribution chain length, ligand density, and ratio enzyme-targeted polymers to efficacy, synergy, selectivity inhibition cell proliferation. The results revealed that employing a moderate length as scaffold allowed an intrinsically high efficacy synergy polymers, contrast single prefer longer...