A5005778977- Semiconductor Lasers and Optical Devices
- Semiconductor Quantum Structures and Devices
- Metabolism, Diabetes, and Cancer
- Photonic and Optical Devices
- Pancreatic function and diabetes
- HIV Research and Treatment
- Radio Frequency Integrated Circuit Design
- Virus-based gene therapy research
- Protein Kinase Regulation and GTPase Signaling
- PI3K/AKT/mTOR signaling in cancer
- Opportunistic and Delay-Tolerant Networks
- Mobile Ad Hoc Networks
- Monoclonal and Polyclonal Antibodies Research
- NF-κB Signaling Pathways
- Silicon Carbide Semiconductor Technologies
- Caching and Content Delivery
- IPv6, Mobility, Handover, Networks, Security
- interferon and immune responses
- Cooperative Communication and Network Coding
- GaN-based semiconductor devices and materials
- Advanced MIMO Systems Optimization
- Inflammatory mediators and NSAID effects
- Cellular transport and secretion
- Advanced Semiconductor Detectors and Materials
- Diabetes and associated disorders
Nagasaki University
2016-2025
Hong Kong Science and Technology Parks Corporation
2024
Chiba University
1996-2022
Tokai University
2003-2022
Oita Medical Center
2021
Hidaka Hospital
2018
Kaetsu University
2018
JNC (Japan)
2017
Tokai University Hospital
2016
Gifu University Hospital
2015
Physical exercise induces translocation of GLUT4 from an intracellular pool to the cell surface in skeletal muscles and increases glucose uptake via insulin-independent pathway. However, molecular mechanism remains be identified. Some studies have suggested that bradykinin is locally released contracting may responsible for increase transport muscles. To determine whether directly triggers translocation, we established L6 myotubes, 3T3-L1 adipocytes, Chinese hamster ovary cells stably...
Stimulation of glucose transport is the main physiological effect insulin in target tissues. This linked to translocation GLUT4 transporter from an intracellular pool cell surface. To elucidate molecular mechanisms involved this effect, we developed a simple direct sensitive method detect immunologically on cDNA containing inserted by c-myc epitope first ectodomain (GLUT4myc) was constructed without disrupting functions and expressed 3T3-L1 Chinese hamster ovary fibroblast cells. In response...
We have developed a highly sensitive and specific solid-phase enzyme immunoassay for 9-deoxy-delta 9,delta 12-dihydroprostaglandin D2 (delta 12-PGJ2) studied the occurrence of this novel PGD2 metabolite in human urine. The assay detected delta 12-PGJ2 over range 2-200 pg, antiserum showed 2% cross-reaction with PGJ2 less than 0.2% other PGs. used purified 12-PGJ2-like immunoreactive substance from Purification consisted chromatographies on Sep-Pak C18 cartridge, silicic acid column,...
Transition state mimetic tripeptide human immunodeficiency virus (HIV) protease inhibitors containing allophenylnorstatine [(2S,3S)-3-amino-2-hydroxy-4-phenylbutyric acid] were synthesized and tested for activity against HIV in vitro. Two compounds, KNI-227 KNI-272, which highly potent with little inhibition of other aspartic proteases, showed the most infectivity cytopathic effect a wide spectrum strains. As target CD4+ ATH8 cells, 50% inhibitory concentrations type 1 LAI (HIV-1LAI),...
In the presence of ouabain, prostaglandin (PG) Ez stimulated a gradual secretion catecholamines from cultured bovine adrenal chromaffin cells.PGEz or ouabain alone evoked marginal secretory response.The synergism was also observed with muscarine.PGE,, like muscarine, induced concentrationdependent formation inositol phosphates: rapid rises in trisphosphate and bisphosphate followed by slower accumulation monophosphate.This effect on phosphoinositide metabolism accompanied an increase...
Abstract Gene editing using CRISPR/Cas9 is a promising method to cure many human genetic diseases. We have developed an efficient system deliver Cas9 into the adeno-associated virus integration site 1 (AAVS1) locus, known as safe harbor, lentivirus and AAV viral vectors, step toward future in vivo transduction. First, we introduced Cas9v1 (derived from Streptococcus pyogenes ) at random genome lentiviral vector. activity was used when N-terminal 1.9 kb, C-terminal 2.3 kb fragments of another...
Tumor necrosis factor α (TNF–α) binding to the TNF receptor (TNFR) initiates apoptosis and simultaneously activates transcription factor, nuclear factor–κB (NF–κB), which suppresses by an unknown mechanism. Pretreatment with TNF–α or interleukin–1β (IL–1β), activated NF–κB in liver, dramatically prevented TNF–α–induced liver–cell D–galactosamine (GalN)–sensitized mice, but not anti–Fas antibody–induced hepatotoxicity. This protective effect of continued for 5 hours after administration, a...
Parkinson disease (PD) is a neurodegenerative disorder characterized by loss of midbrain dopaminergic (DA) neurons. ES cells are currently the most promising donor cell source for cell-replacement therapy in PD. We previously described strong neuralizing activity present on surface stromal cells, named cell–derived inducing (SDIA). In this study, we generated neurospheres composed neural progenitors from monkey which capable producing large numbers DA demonstrated that FGF20, preferentially...
Non‐enzymatic glycosylation (glycation) is a chain of chemical reactions affecting free amino groups in proteins long‐living eukaryotes. It proceeds several steps leading to the consecutive formation Schiff bases, Amadori products and advanced glycation end‐products (AGEs). To our knowledge, this process has not been observed prokaryotes so far. However, present study provides clear‐cut evidence that takes place bacteria despite their short life span. We have detected AGEs recombinant human...
We cloned a novel cDNA derived from the CARD6 gene locus on chromosome 5p12 of 311 amino acids in length. By immunoprecipitation we detected specific binding this CARD6-encoding protein to Nod1 (CARD4), Cardiak (Rip2/Rick), NAC (NALP1/DEFCAP/CARD7), and TUCAN (CARD8/Cardinal/NDPP/Dakar), caspase recruitment domain (CARD)-containing proteins implicated NF-κB caspase-1 activation but not other CARD family proteins. (but proteins) also exhibited opposing effects phosphorylation expression,...
After adding insulin to cells overexpressing the receptor, activity of phosphatidylinositol (PI) 3-kinase in anti-phosphotyrosine immunoprecipitates was rapidly and greatly increased. This enzyme may therefore be a substrate for receptor tyrosine kinase one mediators signal transduction. However, it is unclear whether or not activated directly phosphorylates PI at residue(s) stimulates specific 3-kinase. We reported previously that 85-kDa subunit purified phosphorylated by vitro. To examine...
Abstract Coincubation of neutrophils with TNF inhibited the chemoattractant-directed migration under agarose and enhanced their in multiwell chemotaxis chamber. To assess physiological significance these differing vitro effects, ex vivo investigations were performed using animal models. Neutrophils from peripheral blood rabbits preadministered systemic showed impaired ability to migrate toward chemoattractants vitro. In addition, administration suppressed zymosan-activated plasma-induced...
This study was designed to clarify the contributions of specific neutrophil chemotactic factors (NCF) in accumulation human respiratory tract associated with various diseases. The activity and characteristics NCF bronchoalveolar lavage (BAL) fluid culture media alveolar macrophages obtained from normal volunteers, control patients, patients chronic airway diseases (CAD) idiopathic pulmonary fibrosis (IPF) were examined. BAL volunteers contained comparable interleukin-8 (IL-8) leukotriene B4...
KNI-272 represents a peptide-based protease inhibitor having potent antiretroviral activity against human immunodeficiency virus (HIV) in vitro. The structure contains allophenylnorstatine [(2S,3S)-3-amino-2-hydroxy-4-phenylbutyric acid] with hydroxymethylcarbonyl isostere. We asked whether this experimental anti-HIV agent could exert its vitro the presence of relatively high concentrations fetal calf serum (FCS) and assessed protein-binding properties by using fresh plasma preparations. 50...
Three types of caseinolytic proteases (Pase-A, Pase-B, and Pase-C) were isolated purified from culture supernatants Porphyromonas gingivalis 381 by the combined procedures acetone precipitation, gel filtration, solubilization with octylthioglucoside followed affinity chromatography on arginine-Sepharose 4B, high-performance liquid (HPLC) Biofine IEC-DEAE, HPLC TSK-G4000SW. By sodium dodecyl sulfate-polyacrylamide electrophoresis, Pase-A -B showed diffuse protein bands 105 to 110 72 80 kDa,...
The stereochemical diversity-oriented conformational restriction strategy can be an efficient method for developing specific ligands drug target proteins, especially in cases where neither the bioactive conformation nor pharmacophore is known. To develop potent H3 and H4 receptor antagonists, a series of conformationally restricted analogues histamine with chiral cis- or trans-cyclopropane structure were designed on basis this strategy. These compounds diversity synthesized from versatile...
We have shown previously that insulin stimulated the tyrosine phosphorylation of alpha-type 85-kDa subunit (p85) phosphatidylinositol (PI) 3-kinase in vitro and vivo. In present work, we identified major sites p85 by receptor. [32P]Phosphopeptides obtained from lysylendopeptidase digestion phosphorylated intact cells after treatment were analyzed using reverse-phase high performance liquid chromatography thin layer electrophoresis. The vivo assigned to three phosphopeptides, designated p1,...