A5049016516- Adenosine and Purinergic Signaling
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Cardiac Ischemia and Reperfusion
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- RNA Interference and Gene Delivery
- Cardiovascular Disease and Adiposity
- Cardiac and Coronary Surgery Techniques
- Peptidase Inhibition and Analysis
- Pharmacological Receptor Mechanisms and Effects
- Infective Endocarditis Diagnosis and Management
- Blood groups and transfusion
- Cardiovascular Function and Risk Factors
- Cancer, Hypoxia, and Metabolism
- Cardiac Structural Anomalies and Repair
- RNA regulation and disease
- Lipoproteins and Cardiovascular Health
- Nitric Oxide and Endothelin Effects
- Cardiac Valve Diseases and Treatments
- SARS-CoV-2 and COVID-19 Research
- Mechanical Circulatory Support Devices
- Calcium signaling and nucleotide metabolism
- Renal Diseases and Glomerulopathies
- Complement system in diseases
Gdańsk Medical University
2022-2025
University of Gdańsk
2023
Mitochondrial dysfunction in failing hearts has been described as a driving force for energy deprivation and cardiomyocyte supply-demand imbalance. Isoproterenol (ISO), the β1/β2-adrenergic receptor agonist that leads to myocardial stress mitochondrial damage, is extensively used vitro vivo studies test efficacy of therapeutic strategies heart failure (HF). This study evaluated cell morphology, nucleotide concentrations, function ISO-treated cardiomyocytes stimulated with activators...
Purinergic signalling pathways play a vital role in the biological functions of aortic valve (AV) through nucleotide and adenosine-dependent receptor effects. This study focused on characterizing side-specific purinergic cascade human non-stenotic stenotic AVs, ovine native AVs novel bacterial nanocellulose (BNC) bio-prosthesis an model. Human were collected during replacement surgeries, while came from heart transplant patients. Ovine sourced domestic sheep, BNC prosthesis was implanted...
Introduction Adenosine deaminase (ADA) isoenzymes play a role in microvascular dysfunction following SARS-CoV-2 infection. This study analyzes the mechanisms behind ADA1-dependent endothelial inflammation post-COVID-19 syndrome. We investigated whether immune cells from post-COVID patients could contribute to increased total ADA activity. Additionally, we examined ADA’s enzymatic and extra-enzymatic activities human primary lung (HULECs) stimulated with patients’ serum. Methods results...
Adenosine deaminase (ADA) via two isoenzymes, ADA1 and ADA2, regulates intra- extracellular adenosine concentrations by converting it to inosine. In the central nervous system (CNS), modulates processes of neuroinflammation demyelination that together play a critical role in pathophysiology multiple sclerosis (MS). Except for their catalytic activities, ADA isoenzymes display extra-enzymatic properties acting as an adhesion molecule or growth factor.This study aimed explore distribution...
Endothelial cells are a preferential target for SARS-CoV-2 infection. Previously, we have reported that vascular adenosine deaminase 1 (ADA1) may serve as biomarker of endothelial activation and inflammation, while ADA2 plays critical role in monocyte macrophage function. In this study, investigated the activities circulating ADA isoenzymes patients 8 weeks after mild COVID-19 related them to parameters inflammation microvascular/endothelial Post-COVID revealed microvascular dysfunction...
C4d is the end degradation product of activated complement component C4b that appears during early steps classical and lectin pathways. Within primary sequence C4d, there a reactive thioester group binds covalently to nearby surfaces, thus labeling locations activation. This feature makes target for immunohistochemical staining aimed aid diagnosis of, among others, antibody-mediated rejection transplanted organs, membranous glomerulonephritis, bullous pemphigoid, or inflammatory myopathies....
The disruption of the metabolism extracellular NAD+ and NMN may affect related signaling cascades pathologies, such as cardiovascular or respiratory system diseases. We aimed to study hydrolysis on surface endothelial cells diverse origins with genetically modified nucleotide catabolism pathways. tested lung isolated from C57BL/6 J wild-type (WT) CD73 knockout (CD73 KO) mice, transfected porcine iliac artery cell line (PIEC) human E5NT gene for (PIEC CD73), a mock-transfected control MOCK),...
Chronic hypoxia drives vascular dysfunction by various mechanisms, including changes in mitochondrial respiration. Although endothelial cells (ECs) rely predominantly on glycolysis, is known to alter oxidative phosphorylation, promote stress and induce ECs. Our work aimed analyze the effects of prolonged treatment with hypoxia-mimetic agent CoCl2 intracellular nucleotide concentration, extracellular breakdown, function, nitric oxide (NO) production microvascular Moreover, we investigated how...
Background: Statins and proprotein convertase subtilisin/kexin type 9 inhibitors (PCSK9i) are cornerstones of therapy to prevent cardiovascular disease, acting by lowering lipid concentrations only partially identified pleiotropic effects. This study aimed analyze impacts atorvastatin synthetic peptide PCSK9i on bioenergetics function microvascular endothelial cells cardiomyocytes. Methods: Mitochondrial abundance as well intracellular nucleotides, membrane potential, cytoskeleton structure,...
Experimental models to test the effective protection against cardiac ischemia injury are still challenging in pre-clinical studies. The use of myocardial slices creates a special link between testing isolated cardiomyocytes and whole-heart research. In this work, we investigated effects oxygen deprivation hypoxic chamber treatment with cobalt chloride (CoCl
Endothelial cells are a preferential target for SARS-CoV-2 infection. Previously, we have reported that vascular adenosine deaminase 1 (ADA1) may serve as biomarker of endothelial activation and inflammation, while ADA2 plays critical role in monocyte macrophage function. In this study, investigated the activities circulating ADA isoenzymes patients 8 weeks after mild COVID-19 related them to parameters inflammation microvascular/endothelial dysfunction.PostCOVID revealed microvascular...