A5066646543- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- Ocular Surface and Contact Lens
- Retinopathy of Prematurity Studies
- Advanced Glycation End Products research
- Retinal Diseases and Treatments
- Dermatologic Treatments and Research
- Neonatal Respiratory Health Research
- Ocular Infections and Treatments
- Peripheral Artery Disease Management
- Hematological disorders and diagnostics
- Neuroscience of respiration and sleep
- Respiratory Support and Mechanisms
- Spaceflight effects on biology
- Infectious Diseases and Tuberculosis
- Retinoids in leukemia and cellular processes
- Orthopedic Infections and Treatments
- Renal function and acid-base balance
- Viral Infections and Outbreaks Research
- Thermal Regulation in Medicine
- Inhalation and Respiratory Drug Delivery
- Coenzyme Q10 studies and effects
- Mosquito-borne diseases and control
- Cardiac, Anesthesia and Surgical Outcomes
Minnesota Lions Eye Bank
2008-2020
University of Iowa
2014-2019
Christie's
2015
University of New Mexico
1996
Ross Laboratories (United States)
1991
Harborview Medical Center
1991
Acute Respiratory Distress Syndrome (ARDS) is characterized by lung injury and damage to the alveolar type II cells. This study sought determine if endogenous surfactant altered in ARDS. Bronchoalveolar lavage was performed patients at-risk develop ARDS (AR, n = 20), with (A, 66) normal subjects (N, 29). The crude pellet analyzed for total phospholipids (PL), individual phospholipids, SP-A, SP-B, minimum surface tension (STmin). PL decreased both AR A (3.48 +/- 0.61 2.47 0.40 mumol/ml,...
Purpose: To characterize changes in the energy-producing metabolic activity and morphologic ultrastructure of corneal endothelial cells associated with diabetes mellitus. Methods: Transplant suitable corneoscleral tissue was obtained from donors aged 50 to 75 years. We assayed 3-mm punches endothelium-Descemet membrane for mitochondrial respiration glycolysis using extracellular flux analysis oxygen pH, respectively. Transmission electron microscopy used assess qualitative quantitative...
Purpose: To quantify changes in endothelial cell density (ECD) of donor corneal tissue relation to the presence or absence a medical history diabetes mellitus diagnosis, treatment, and complications. Methods: A retrospective review was performed for all corneas collected at Iowa Lions Eye Bank between January 2012 December 2015. For purposes analysis, were divided into 4 groups: nondiabetic, non–insulin-dependent diabetic, insulin-dependent diabetic without complications due diabetes, with...
The objective of this study was to characterize the proteome corneal endothelial cell layer and its basement membrane (Descemet membrane) in humans with various severities type II diabetes mellitus compared controls, identify differentially expressed proteins across a range diabetic disease that may influence health. Endothelium-Descemet complex tissues were peeled from transplant suitable donor corneas. Protein fractions isolated each sample subjected multidimensional liquid chromatography...
Purpose: To determine the concentration of amphotericin B that would be both effective against Candida albicans contamination and safe for corneal endothelial cells (CECs) in cold storage conditions. Methods: Triplicate media cultures were inoculated with 10 5 colony-forming units (CFUs)/mL C. (American Type Culture Collection 10231), supplemented (0–20 μg/mL), stored conditions (2°C–8°C) 72 hours, analyzed quantitatively CFUs. each sample was determined initially after 6, 24, 48, hours...
Purpose: To assess surgeon satisfaction with precut corneal tissue from 1 eye bank for Descemet stripping automated endothelial keratoplasty (DSAEK). Surgical techniques and predictors of procedural success were also examined. Methods: A 19-question survey was completed by 53 surgeons around the United States 197 DSAEK cases using prepared allograft Iowa Lions Eye Bank. Surgeries performed between April December 31, 2006; surveys retrospectively within a few weeks surgery. Results: Tissue...
Purpose.: We characterized mitochondrial respiration and glycolysis activity of human corneal endothelium, compared metabolic between central peripheral regions. Methods.: Endothelial keratoplasty-suitable corneas were obtained from donors aged 50 to 75 years. The endothelium–Descemet membrane complex (EDM) was isolated, 3-mm punches Endothelium–Descemet assayed for (oxygen consumption) (extracellular acidification) using an extracellular flux analyzer. Enzymatic (citrate synthase, glucose...
Purpose: To determine how the Rho kinase inhibitor, ripasudil, affects metabolic function and cell viability in donor human corneal endothelial cells (HCECs). Methods: Endothelial cell-Descemet membrane (EDM) tissues were treated with 10 μM ripasudil assayed for mitochondrial glycolytic activity using extracellular flux analysis then compared to untreated controls. In addition, EDM a 24-h treatment control exposed 1 staurosporine induce apoptosis analyzed necrosis assays. Results:...
To compare Descemet membrane endothelial keratoplasty (DMEK) outcomes using nondiabetic grafts in diabetic and recipients. All eyes that underwent DMEK between February 2013 October 2016 (follow-up ≥3 months, without prior keratoplasty) were included. Recipients divided into (insulin dependent [IDDM] or noninsulin [NIDDM]) groups. Main outcome measures included postoperative visual acuity, rebubble procedure rates, graft failure rates. Of 334 (243 subjects) for analysis, 63 (18.8%) from At...
The purpose of this study was to determine the in vitro and ex vivo susceptibility human corneal cells West Nile virus (WNV) infection evaluate ability disseminate corneas infected mice. Human epithelial were challenged with WNV, incubated for 1-6 days, tested evidence WNV infection. Viral RNA antigen detected at every time point, reached a peak titer 2.5 × 107 plaque-forming units (pfu)/mL 3 days postinoculation (PI). Corneas procured from donors culture dishes containing 1-5 WNV. detected,...
Purpose: To assess how trypan blue staining affects Descemet membrane endothelial keratoplasty (DMEK) graft visibility and corneal cell (CEC) mitochondrial respiration. Methods: DMEK grafts (n = 20) were stained with 0.06% for 1, 3, 5, or 10 minutes. Each was injected into an artificial anterior chamber. Surgery simulated tapping sweeping motions on the surface injections of balanced salt solution (BSS). Graft assessed at 10, 20, 30 Effects respiration using primary CECs cultured from donor...
Purpose: To develop a method based on identification of the widest region surgical limbus that can yield quick and accurate orientation excised human donor corneas. Methods: Corneoscleral tissue from donors 49 to 75 years old was marked at temporal sclera time recovery. Digital images obtained 20 corneas stored in viewing chambers, retroilluminated viewed endothelial side, were used quantify per-degree radial width limbus, defined as distance scleral spur clear cornea. evaluate differences...