A5076442527- Neuroinflammation and Neurodegeneration Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Immune cells in cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Metabolism and Genetic Disorders
- RNA modifications and cancer
- Immune Response and Inflammation
- Adipose Tissue and Metabolism
- Protein Structure and Dynamics
- Protein Tyrosine Phosphatases
- S100 Proteins and Annexins
- Inflammation biomarkers and pathways
- Neurological diseases and metabolism
- Peripheral Neuropathies and Disorders
- Genomics and Chromatin Dynamics
- Cholesterol and Lipid Metabolism
- Neurogenetic and Muscular Disorders Research
- Advanced Glycation End Products research
- Galectins and Cancer Biology
- Endoplasmic Reticulum Stress and Disease
- Shoulder and Clavicle Injuries
- Macrophage Migration Inhibitory Factor
- Sphingolipid Metabolism and Signaling
- Dental Implant Techniques and Outcomes
Columbia University Irving Medical Center
2024
Howard Hughes Medical Institute
2022-2023
Princeton University
2021-2023
KU Leuven
2004-2019
Columbia University
2019
Harvard University
2017
Brigham and Women's Hospital
2017
Phase separation of biomolecules into condensates has emerged as a mechanism for intracellular organization and affects many processes, including reaction pathways through the clustering enzymes pathway intermediates. Precise rapid spatiotemporal control reactions by requires tuning their sizes. However, physical processes that govern distribution condensate sizes remain unclear. Here we show both native synthetic display an exponential size distribution, which is captured Monte Carlo...
The nucleolus is the largest biomolecular condensate and facilitates transcription, processing, assembly of ribosomal RNA (rRNA). Although nucleolar function thought to require multiphase liquid-like properties, fluidity its connection highly coordinated transport biogenesis subunits are poorly understood. Here, we use quantitative imaging, mathematical modeling, pulse-chase nucleotide labeling examine material properties rRNA dynamics. mobility several orders magnitude slower than that...
Currently CAD-CAM systems are rapidly gaining importance in dental practice as some of their products aim to combine aesthetics with strength and free metals. This study reports on the crown adaptation, marginal fit clinical behaviour 300 all-ceramic full coverage restorations (Procera, Gothenburg, Sweden) placed one centre followed up 5 years. The coping adaptation before after luting was determined by direct measurement well sectioning a laboratory study. Three hundred all-ceram were...
Abstract The nucleolus facilitates transcription, processing, and assembly of ribosomal RNA (rRNA), the most abundant in cells. Nucleolar function is facilitated by its multiphase liquid properties, but nucleolar fluidity connection to ribosome biogenesis remain unclear. Here, we used quantitative imaging, mathematical modeling, pulse-chase nucleotide labelling map rRNA dynamics. Inconsistent with a purely diffusive process, steadily expands away from transcriptional sites, moving slow...
The functional diversity and molecular adaptations of reactive microglia in the chronically inflamed central nervous system (CNS) are poorly understood. We previously showed that mice lacking multifunctional protein 2 (MFP2), a pivotal enzyme peroxisomal β‐oxidation, persistently accumulate myeloid cells gray matter CNS. Here, we show increased numbers solely derive from proliferation resident not infiltrating monocytes. defined signature Mfp2 −/− by gene expression profiling after acute...
Microglia play a central role in most neurological disorders, but the impact of microgliosis on brain environment and clinical functions is not fully understood. Mice lacking multifunctional protein-2 (MFP2), pivotal enzyme peroxisomal β-oxidation, develop fatal disorder characterized by motor problems similar to milder form MFP2 deficiency humans. The hallmark disease mice chronic proliferation microglia brain, molecular pathomechanisms that drive rapid deterioration human remain unknown....
Oxysterols and cholestenoic acids are oxidised forms of cholesterol with a host biological functions. The possible roles oxysterols in various neurological diseases makes the analysis these metabolites central nervous system particular interest. Here, we report identification quantification panel twelve sterols mouse cerebrospinal fluid (CSF) using liquid chromatography-mass spectrometry exploiting enzyme assisted derivatisation for sterol technology. We found low levels CSF range...
Large-scale genetic studies have identified numerous risk factors that suggest a central role for innate immune cells in susceptibility to Alzheimer’s disease (AD). CD33, an immunomodulatory transmembrane sialic-acid binding protein expressed on myeloid cells, was as one such factor associated with disease. Several explored the molecular outcomes of variation at CD33 locus. It has been determined variant AD increases expression large isoform (CD33M) and alters its biological functions. is...
It is becoming evident that microglia, the resident immune cells of central nervous system (CNS), are active contributors in neurological disorders. Nevertheless, impact microgliosis on neuropathology, behaviour and clinical decline neuropathological conditions remains elusive. A mouse model lacking multifunctional protein-2 (MFP2), a pivotal enzyme peroxisomal β-oxidation, develops fatal disorder characterized by motor problems similar to milder form human disease. The molecular mechanisms...
Abstract Microglia are resident immune cells of the central nervous system (CNS). The exact role microglia in physiopathology CNS disorders is not clear due to lack tools discriminate between and infiltrated innate cells. Here, we present a novel reporter mouse model targeting microglia-specific marker (TMEM119) for studying function health disease. By placing cassette (GSG-3xFlag-P2A-tdTomato) coding sequence exon 2 3’UTR Tmem119 gene using CRISPR/Cas9 technology, generated Tmem119-tdTomato...
Large-scale genetic studies have identified numerous risk factors that suggest a central role for innate immune cells in susceptibility to Alzheimer's disease (AD). CD33, an immunomodulatory transmembrane sialic acid binding protein expressed on myeloid cells, was as one such factor associated with disease. Several explored the molecular outcomes of variation at