A5043247786- Gut microbiota and health
- Diet and metabolism studies
- Helicobacter pylori-related gastroenterology studies
- Gastrointestinal motility and disorders
- Clostridium difficile and Clostridium perfringens research
- Cancer Immunotherapy and Biomarkers
- Microscopic Colitis
- Liver Disease Diagnosis and Treatment
- Bariatric Surgery and Outcomes
- Tuberculosis Research and Epidemiology
- Psychological Well-being and Life Satisfaction
- Bladder and Urothelial Cancer Treatments
- Probiotics and Fermented Foods
- Fire effects on ecosystems
- Metabolomics and Mass Spectrometry Studies
- Immune Cell Function and Interaction
- Rangeland and Wildlife Management
- Forest ecology and management
- Celiac Disease Research and Management
- Streptococcal Infections and Treatments
- Salmonella and Campylobacter epidemiology
- Pneumocystis jirovecii pneumonia detection and treatment
- Nutritional Studies and Diet
- Viral gastroenteritis research and epidemiology
- Bee Products Chemical Analysis
Wilfrid Laurier University
2019-2024
The University of Sydney
2019-2024
St. Mary's University
2023
Princess Alexandra Hospital
2015-2022
Translational Research Institute
2015-2022
The University of Queensland
2014-2022
Health & Life (Taiwan)
2022
King Edward Memorial Hospital
2021
Edith Cowan University
2021
Princess Alexandra Hospital
2020
Cigarette smoking is a known risk factor in number of gastrointestinal (GI) diseases which the microbiota implicated, including duodenal ulcer and Crohn's disease. Smoking has potential to alter microbiota; however, date, impact on mucosa-associated (MAM), particularly that upper GI tract, remains very poorly characterised. Thus, we investigated small intestinal MAM. A total 102 patients undergoing endoscopy for assessment symptoms, iron deficiency, or disease, but without identifiable...
Abstract Secretory IgA is a key mucosal component ensuring host-microbiota mutualism. Here we use nutritional geometry modelling in mice fed 10 different macronutrient-defined, isocaloric diets, and identify dietary protein as the major driver of secretory production. Protein-driven induction not mediated by T-cell-dependent pathways or changes gut microbiota composition. Instead, high produces significantly higher quantities extracellular vesicles, compared to those high-carbohydrate...
Objective Functional dyspepsia (FD) is a complex disorder, with debilitating epigastric symptoms. Evidence suggests alterations in gastrointestinal (GI) motility, visceral hypersensitivity, permeability and low-level immune activation the duodenum may play role. However, we still have relatively poor understanding of how these factors interact to precipitate onset FD symptoms which are frequently meal related. The duodenal microbiota, combination specific dietary substrates, be important...
Summary Background The mucosa‐associated microbiota appears to be highly relevant host–microbe interactions in the gastrointestinal (GI) tract. Thus, precise characterisation of may provide important insights for diagnostic and therapeutic development. However, technical reasons, mucosal biopsies taken during standard endoscopic procedures are potentially contaminated by GI luminal contents. Aim To develop validate a biopsy device that minimises contamination sampling microbiota. Methods A...
Summary Background Small intestinal bacterial overgrowth may play a role in gastrointestinal and non‐gastrointestinal diseases. Aims To use quantitative polymerase chain reaction (qPCR) to determine compare loads of duodenal biopsies asymptomatic controls, patients with functional disorders (FGIDs) inflammatory bowel disease (IBD) including ulcerative colitis (UC) Crohn’s (CD). define effects gastric acid inhibition on load, explore links load symptoms response standardised nutrient...
Human gut microbiome structure and emergent metabolic outputs impact health outcomes. However, what drives such community characteristics remains underexplored. Here, we rely on high throughput genomic reconstruction modeling, to infer the attributes nutritional requirements of 816 strains, via a framework termed GEMNAST. This has been performed in terms group human vitamins examine role vitamin exchanges have at different levels organization. We find that only 91 strains can satisfy their...
Metagenomics has rapidly advanced our inventory and appreciation of the genetic potential inherent to gut microbiome. However it is widely accepted that two key constraints further dissection microbiota host-microbe interactions have been inability recover new isolates from human gut, paucity genetically tractable microbes. To address this challenge we developed a modular RP4 mobilisable recombinant vector system an approach termed metaparental mating support rapid directed isolation...
Frequently, patients with functional gastrointestinal disorders (FGIDs) report intolerance of wheat products. We compared symptoms, sensory function, psychiatric comorbidities, gut-homing immune cells, and duodenal mucosa-associated microbiome (d-MAM) in FGID controls without self-reported sensitivity (SR-NCWS). recruited 40 20 referred by GPs for treatment. Gastrointestinal/extraintestinal visceral psychological SR-NCWS were assessed a standardized approach. Peripheral gut homing T-cells...
OBJECTIVES: Chronic liver disease (CLD) is associated with both alterations of the stool microbiota and increased small intestinal permeability. However, little known about role mucosa-associated (MAM) in CLD. The aim this study was to evaluate relationship between duodenal MAM permeability severity METHODS: Subjects CLD a disease-free control group undergoing routine endoscopy underwent biopsy assess by 16S rRNA gene sequencing. Small assessed dual sugar (lactulose: rhamnose) assay. Other...
Honey bees (Apis mellifera) face increasing threats to their health, particularly from the degradation of floral resources and chronic pesticide exposure. The properties honey bee gut microbiome are known both affect be affected by health. Using samples healthy hives showing signs stress a single apiary with access same resources, we profiled antimicrobial activity chemical determined bacterial fungal hive environment. We found was significantly more active than stressed hives, increased...
New therapies to control tuberculosis are urgently required because of the inability only available vaccine, BCG, adequately protect against tuberculosis. Here we demonstrate that proteins Mycobacterium sulfate-assimilation pathway (SAP) represent major immunogenic targets bacillus, as defined by strong T-cell recognition both mice and humans infected with M. SAP displayed increased expression when was resident within host cells, which may account in part for their ability stimulate anti-M....
Abstract Background and aims Functional dyspepsia (FD) is a highly prevalent disorder of gut-brain interaction (DGBI) that associated with an altered duodenal microbiota, unexplained low grade inflammation intestinal permeability. This study aimed to investigate if novel FD-derived bacterial isolates elicited immune responses in FD the capacity immune-stimulating isolate, AGIRA0003 breach epithelial barrier. Methods Bacterial lysates were investigated for reactivity using immunoblotting...
Abstract Background The human gut microbiome plays a pivotal role in health and disease, influenced significantly by diet, particularly through the intake of digestion-resistant carbohydrates (DRCs). Emerging evidence underscores potential DRC supplementation modulating towards health-promoting metabolic outputs, notably fermentation DRCs into short-chain fatty acids (SCFAs) including butyrate. However, effectiveness such interventions is hindered inherent complexity microbial communities...